Tender For Fsl Kit Items With Accessories; 1 List Of Kits Items With Accessories 2 Automated Dna Extraction Kit 1. All Reagents And Plastic Ware For The Purification Of Gdna Should Be Included With The Kits. 2. Kits Based On Pre-Dispensed Cartridges Format, Should Utilize Magnetic Bead-Based Technology For Efficient Dna Purification From All Kind Of Common & Challenging Samples Like Body Fluids, Blood, Vaginal And Semen Stains, Tissue, Bone, Tooth, Hair, Chewing Gum, Cigarette Butts Samples Etc. 3. The Required Reagents For Dna Extraction Such As Lysis Buffer, Proteinase-K / Dtt And Or Anything Which Is Part Of Recommended Protocol Of The Manufacturer Including Suitable Thermomixer ( 04 Nos With The First Order ) Should Be Supplied 4. The Kit Should Be Compatible With Auto Mate Express / Ez1 Advanced Instrument / Maxwell Fsc Instrument 5. Manufacturing Of Provided Kit Should Follow Iso 18385 Certified Guidelines And Kit Should Be Hid Validated 6.All Reagents And Plastic Ware For The Purification Of Gdna Should Be Included With The Kits 3 Dna Extraction Kit For Hid Nimbus Presto Automated Dna Extraction System ( Routine Forensic Sample ) 1. The Kit Should Be Validated In Accordance Scientific Working Group On Dna Analysis Methods ( Swgdam ) Guideline For Use In Human Identification Testing ( Hid Validated ) . 2.All The Forensic Validated Reagent And Recommended Plastic Ware For The Purification Of Gdna Should Be Supplied Along With The Kits 3.Kit Should Utilize Integrated Magnetic Particle-Based Technology For Dna Purification With Full Optimized And Validated Scripts Compatible With Hid Nimbus Presto 4.Kit Should Utilize Optimized And Validated Scripts For Efficient Dna Purification From Routine Forensic Sample Types, Including: Body Fluids ( Blood, Saliva, Semen ) Stains And Swabs Of Body Fluids, Hair Roots &Touch / Trace Samples. 5.Required Reagent For Dna Extraction Such As Lysis Buffer, Magnetic Particles, And Wash Buffers Concentrate & Elution Buffer Should Be Included In The Kit. 6.Required Plastic Wares For Dna Extraction And Purification Should Be Supplied Along With The Kit. 7.Kit Should With Compatible With Available Hidnimbus Presto System And Required Documentation Should Be Provided And Scripts Should Be Available On The System. 8.Manufacturing Of Provided Kit Should Follow Iso 18385 Certified Guidelines ( Forensic Grade Quality Assurance Production Environment In Accordance With Iso18385:2016 ) . 4 Dna Extraction Kit For Hid Nimbus Presto Automated Dna Extraction System ( Difficult Forensic Sample ) 1. The Kit Should Be Validated In Accordance With The Dna Advisory Board ( Dab ) Quality Assurance Standards And Guidelines From The Scientific Working Group On Dna Analysis Methods ( Swgdam ) For Use In Human Identification Testing ( Hid Validated ) . 2. All The Forensic Validated Reagent And Recommended Plastic Ware For The Purification Of Gdna Should Be Supplied Along With The Kit 3. Kit Should Utilize Integrated Magnetic Particle-Based Technology For Dna Purification In An Automated Liquid Handling Platform With Full Optimized And Validated Scripts Compatible With Hid Nimbus Presto 4. All The Reagent And Plastic Ware For The Purification Of Dna Should Be Supplied Along With The Kit 5. Kit Should Utilize Magnetic Bead-Based Technology For Efficient Dna Purification From Difficult Forensic Sample Types, Including: Bone, Teeth, Adhesive Substrates ( Such As Cigarette Butts, Chewing Gum, And Tape Lifts ) & Touch / Trace Samples 6. Required Reagent For Dna Extraction Such As Lysis Buffer, Specific Lysis Buffer For Bone Tooth And Challenging Samples, Magnetic Particles, Wash Buffer A Concentrate, Wash Buffer B Concentrate, Elution Buffer & Proteinase K Should Be Included In The Kit 7.The Required Plastic Wares For Dna Extraction And Purification Should Be Supplied Along With The Kit 8.Kit Quoted Should With Compatible With Available Hid Nimbus Presto System And Scripts Should Be Available On The System. 9.Manufacturing Of Provided Kit Should Follow Iso 18385 Certified Guidelines ( Forensic Grade Quality Assurance Production Environment In Accordance With Iso18385:2016 ) . 5 Dna Quantification Kit 1.The Kit Should Provide All The Components And Standards To Quantify Human Genomic Dna, Male Dna, And Determine Degradation And Inhibition Index Of The Sample Using Qpcr. 2.Detection Of Amplification For All Targets Using The Kit Should Be Performed Using Taqman / Hydrolysis Probe Chemistry. 3.Detection Limit Of The Kit Should Be1.0Pg / Μl Or Better 4.The Quantified Dna Should Either Be Suitable For Autosomal Str Based Or Y- Chromosome Specific Str Based Testing. 5.Kit Should Be Compatible With Abi 7500 Real Time Pcr Systems / Quantstudio™ 5 Real-Time Pcr System.Required Suitable All Calibration Kit Should Be Provided By The Vendor. 6.Kit Should Be In Small Aliquots Of 100 Or 200 Or 400Reactions. 7. Manufacturing Of Provided Kit Should Follow Iso 18385 Certified Guidelines And Kit Should Be Hid Validated 6 Genotyping Kit For Autosomal Str Markers 1.The Autosomal Str Kit Should Contain All Reagents For The Multiplex Amplification Of At Least22 Or More Str Loci With All The 20 Codis Core Loci And Should Include Se33, Penta D And Penta E ( At Least Two Of These Should Be Part Of Single Multiplex ) Along With Published Forensic Validation As Per Swgdam Guideline In Pubmed Journal. 2.Kit Should Also Include One Or More Y Indel / Y Str And Amelogenin Marker. 3.Multiplex Kit Should Support 6 Dye Based Improved Chemistry And Resistance For Pcr Inhibitors. . 4.Storage At 2-8 Degree After Initial Use. 5.Kit Should Be Compatible With Ce System Abi 3500 Xl. 6.Suitable Allelic Ladder, Control Dna, Suitable Size Standard, Pcr Grade Water Must Be Included In The Kit. 7.Appropriate Matrix Calibration Standard ( 05 Sets ) , Panels, Bin Sets Compatible With Gene Mapper Id-X 1.6 8.The Kit Should Be Highly Sensitive To Detect ≤ 0.5 Ng Of Dna Or Better. 9.Manufacturing Of Provided Kit Should Follow Iso 18385 Certified Guidelines And Kit Should Be Hid Validated. 10.Kit Should Be Supplied In Small Aliquots Of 100 / 200 / 400 Reactions. 7 Genotyping Kit For Y Chromosome Markers 1.Kit Should Work With 5-Dye / 6-Dye Chemistry For Analysis Of 23 Or More Y Chromosome Str Markers Along With Published Forensic Validation As Per Swgdam Guideline In Pubmed Journal. 2.Reaction Mix Should Provide Excellent Sensitivity And Robustness Against Inhibitors, Even In High Female Backgrounds 3.Storage At 2-8 Degree After Initial Use 4.Kit Should Include Allelic Ladder, Control Dna, Pcr Grade Water And Size Standard Along With Master Mix And Primer Mix With Pack Size Of 100 Reactions Or 200 Reactions 5.Appropriate Matrix Calibration Standard ( 5 Sets ) , Panels, Bin Sets Compatible With Gene Mapper Id-X 1.6 6.Kit Should Be Compatible With Ce System 3500 Xl 7.The Kit Should Be Highly Sensitive To Detect ≤200Pg Or Better. 8.Manufacturing Of Provided Kit Should Follow Iso 18385 Certified Guidelines And Kit Should Be Hid Validated. 8 Mitochondrial Sequencing Kit 1.Kit Should Be Sensitive For Identification Of Highly-Degraded Samples And Other Difficult Samples And Should Work In Cases With Failure Of Autosomal Str Amplification. 2.Kit Should Be Mitochondrial Sequencing Of Hvi And Hvii Region. 3.Kit Sensitivity- Equal To Or Less Than 125 Picograms Or More 4.Kit Should Be Supplied In Small Aliquots Of 50 / 100 Reactions 5.Kit Should Have All Required Chemical And Kit For Extracted Dna To Mitochondrial Sequencing Of Hvi And Hvii. 9 X- Str Kit 1.Kit Should Have The Minimum 12 Or More X-Str Markers 2.Kit Should Work On Abi 3500Xl Genetic Analyzer. 3.Kit Sensitivity- Equal To Or Less Than 200 Picograms 4.Kit Should Be Supplied With Appropriate Quantity Of Control Dna, Size Standard, Allelic Ladder And Pcr Grade Water ( Cost Of Control Dna, Size Standard, Pcr Grade Water And Allelic Ladder Should Be Included In The Kit ) 5.Suitable Matrix Standards Should Be Supplied With This Supply Order. 6.Kit Should Be Supplied In Small Aliquots Of 50 / 100 / 200 Reactions 10 Kits For Str Analysis By Ngs 1.Kitsshouldhave Str Pannel 35 Ormore Markers 2.Barcodeadaptor 1-32 Set Forautomationprocess, 3.Library Prepation Kits Forautomationprocess 4.Libray Quantitation Kit 5.Template Prepation Kits Forautomationprocess 6.Chips-530 7.Sequencing Kits & Reagent And Other Requirements For Automation Process. 11 Kits For Whole Mitochondrial Sequencing Analysis By Ngs 1.Kitsshouldhave Whole Mitochondrial Sequencing Panel 2.Barcodeadaptor 1-32 Set Forautomationprocess, 3.Library Prepation Kits Forautomationprocess 4.Libray Quantitation Kit 5.Template Prepation Kits Forautomationprocess 6.Chips-530 7.Sequencing Kits Ngs & Reagent And Other Requirements For Automation Process. 12 Rapid Body Fluid Identification Kit For Human Blood ( Forensic Validated Kit ) 1.Kit Should Be Forensically Validated 2.Kit Should Be Based Onhuman Hemoglobin ( Hb ) 3.Kit Should Be Specific For Human Blood Detection 4.The Cassettes Should Have A Feature Of Possible Semi-Quantitative Analysis Of Body Fluid. 5.The Detection Should Be Immunochromatography Based With High Specificity And High Sensitivity ( >99.5% ) Towards Detected Body Fluid. 6.The Kits Supplied Have Peer Reviewed Publications In Forensic Journals In International Journals. 13 Rapid Body Fluid Identification Kit For Human Semen ( Forensic Validated Kit ) 1.Kit Should Be Forensically Validated 2.Kit Should Be Based On Prostate-Specific Antigen ( Psa ) 3.Kit Should Be Specific For Human Semen Detection 4.The Cassettes Should Have A Feature Of Possible Semi-Quantitative Analysis Of Body Fluid. 5.The Detection Should Be Immunochromatography Based With High Specificity And High Sensitivity ( >99.5% ) Towards Detected Body Fluid. 6.The Kits Supplied Have Peer Reviewed Publications In Forensic Journals In International Journals. 14 Rapid Body Fluid Identification Kit For Human Saliva ( Forensic Validated Kit ) 1.Kit Should Be Forensically Validated 2.Kit Should Be Based Onhuman Alpha Amylase 3.Kit Should Be Specific For Human Saliva Detection 4.The Cassettes Should Have A Feature Of Possible Semi-Quantitative Analysis Of Body Fluid. 5.The Detection Should Be Immunochromatography Based With High Specificity And High Sensitivity ( >99.5% ) Towards Detected Body Fluid. 6.The Kits Supplied Have Peer Reviewed Publications In Forensic Journals In International Journals. 15 Anode Buffer Container ( Abc ) 3500 Series, 4 Per Pack 16 Cathode Buffer Container ( Cbc ) 3500 Series, 4 Per Pack 17 3500 Pop-4Tm Polymer Ea 384 Sample, 384 Samples 18 Hi-Di Formamide 25Ml Bottle, 384 Samples 19 Conditioning Reagent, 3500 Series, 20 Septa For 3500 / 3500Xl Genetic Analyzers, 96 Well, 20 Per Pack 21 24 Capillary Array 36 Cm Compatible With 3500Xl , One Set 22 Microamp™ 96-Well Support Base, 10 Per Pack 23 Microamp™ Adhesive Film Applicator, 5 Per Pack 24 Retainer & Base Set ( Standard ) For 3500 / 3500Xl Genetic Analyzers, 4 Per Pack 25 Roi & Background Calibration Kit Compatible With Abi 7500 Real Time Pcr Systems / Quantstudio™ 5 Real-Time Pcr System 26 Quantstudio™ 3 / 5 10-Dye Spectral Calibration Kit
