Tender For Dna Sample , Cost Of Whole Genome Resequencing Of Lentil (Lens Culinaris)Mutants.: Extraction Of Genomic Dna With Assured Quality In Nanodrop Followed By Very Good Quality Library Preparation And Sequencing. At Least 90% Of The Trimmed Seq

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Dna Sample , Cost Of Whole Genome Resequencing Of Lentil ( Lens Culinaris ) Mutants.: Extraction Of Genomic Dna With Assured Quality In Nanodrop Followed By Very Good Quality Library Preparation And Sequencing. At Least 90% Of The Trimmed Sequenced Bases Must Be Of Q30. The Trimmed Sequencing Data Must Be 80Gb ( 20X ) Or Above The Lentil Reference Genome ( Genome Size, 4Gb ) ; Assembling Andpairwise Comparison With The Reference Genome; Snp Calling:Snps And Indels Are To Be Detected Using Soap Snp Andsoap Indel, Respectively. Snp Annotation ( Including Synonymousand Non-Synonymous Snp Mutations ) And Indelannotation Must Be Performed According To The Lens Culinarisreference Genome With The Assigned Linkage Group. Free Pick Up Of Samples In Dry Ice From Bckv Must Be Assured In The Quotation.