E Tender For Procurement Of Some Kits For Two Years From The Date Of Award Of Contract ( Aoc ) And Its Extension, If Required , Rh +K Phenotyping Glass Bead Cassettes Technical Specification: 1.Cassttes Should Contain 6 Columns Filled With Glass Bead As Filtration Media 2.1St Column To 5Th Column Should Be Prefilled With Monoclonal Antisera Such As Anti C, Anti E, Anti C, Anti E, Anti K Respectively And 6Th Column Is Control Column. 3.Peg Should Be Present In The Columns As Enhancer To Increase Uptake Of Antibody 4. Assay Time Should Be Not More Than 5 Minutes 5. Mixed Field Reaction / Double Cell Population Should Be Detected , Rh +K Phenotyping Gel Card Technical Specification: 1. Cassettes Should Contain 6 Columns Filled With Sephadexgelas Filtration Media 2. 1St Column To 5Th Column Should Be Prefilled With Monoclonal Antisera Such As Anti C, Antic, Anti E, Anti E, Anti K Respectively And 6Th Column Is Control Column. 3. Assay Time Should Be Not More Than 10 Minutes 4. Mixed Field Reaction / Double Cell Population Should Be Detected , Ahg Poly Specificglass Bead Cassettes Technical Specification: 1. Cassettes Should Contain 6 Columns Filled With Glass Beads Filtration Media 2. All Column Should Prefilled Withahg Reagent ( Anti Igg , Anti C3d ) 3. Assay Time Should Be Not More Than 15 Minutes For Ict 4. Mixed Filled Reaction / Double Cell Population Should Be Detected , Blood Grouping ( Forward & Reverse Grouping ) Glass Bead Cassettes Technical Specification: 1. Cassettes Should Contain 6 Columns Filled With Glass Bead As Filtration Media 2. 1St Column To 3Rd Column Should Be Prefilled With Monoclonal Antisera Such As Anti A, Anti B, Anti D Respectively And 4Th Column Is Control Column . 5Th And 6Th Columns Are For Doing Reverse Grouping 3. Peg Should Be Present In The Columns As Enhancer To Increase Uptake Of Antibody 4. Room Temperature Incubation Should Not Be Required 5. Centrifugation Time Should Not More Than 5 Minutes 6. Mixed Field Reaction / Double Cell Population Should Be Detected , Blood Grouping ( Forward And Reverse ) Gel Card Technical Specification: 1. Cardshould Contain 6 Columns Filled With Sephadex Gel As Filtration Media 2. 1St Column To 3Rd Column Should Be Prefilled With Monoclonal Antisera Such As Anti A, Anti B, Anti D Respectively And 4Th Column Is Control Column . 5Th And 6Th Columns Are For Doing Reverse Grouping 3. Room Temperature Incubation Should Not Be More Than 10 Minutes 4.Centrifuge Time Should Not Be More Than 10 Minutes 5. Mixed Field Reaction / Double Cell Population Should Be Detected , Monoclonal Antisera For Extended Rh+K Phenotyping Anti C Technical Specification: Anti C Sera Should Be Monoclonal Anti C Sera Should Be Compatible For Conventional Test Tube Method Titre Of The Antisera Should Be More Than 1:64 , Monoclonal Antisera For Extended Rh+K Phenotyping Anti C Technical Specification: Anti C Sera Should Be Monoclonal Anti C Sera Should Be Compatible For Conventional Test Tube Method Titre Of The Antisera Should Be More Than 1:64 , Monoclonal Antisera For Extended Rh+K Phenotyping Anti E Technical Specification: Anti E Sera Should Be Monoclonal Anti E Sera Should Be Compatible For Conventional Test Tube Method Titre Of The Antisera Should Be More Than 1:64 , Monoclonal Antisera For Extended Rh+K Phenotyping Anti E Technical Specification: Anti E Sera Should Be Monoclonal Anti E Sera Should Be Compatible For Conventional Test Tube Method Titre Of The Antisera Should Be More Than 1:64 , Monoclonal Antisera For Extended Rh+K Phenotyping Anti K ( Kell ) Technical Specification: Anti Ksera Should Be Monoclonal Anti Ksera Should Be Compatible For Conventional Test Tube Method Titre Of The Antisera Should Be More Than 1:64 , Panel For Antibody 3 Cell Screening Applicable To Conventional Test Tube Method And Glass Bead Cassettes Technical Specification: 1. Cell Panel Should Be 2%-5% Of Rbc Suspended In Saline 2. Cell Panel Should Be Compatible With Both Conventional Test Tube Method And Glass Bead Cassettes Of Column Agglutination Technology For Doing Antibody Screening Test 3. Cell Panel Should Contain Rbc With The Antigens Of Extended Rh, Kell, Duffy, Kidd, Lewis, Mns Etc Blood Group System 4. Dose Depended Antigens ( Like Mns, Duffy Etc ) Should Be Present In Homozygous As Per Criteria Of Antibody Screening Test 5. Cell Panel Should Be Compatible For Enzyme Treatment 6. Pack Size Should Be 3X3ml Or / And 3X10 Ml To Minimize Wastage Depending On The Requirement Of Antibody Screening Test , Panel For Antibody 3 Cell Screening Applicable To Gel Card Technical Specification: 1.Cell Panel Should Be 0.8%-1%Of Rbc Suspended In Low Ionic Strength Saline 2. Cell Panel Should Be Compatible With Gel Cardof Column Agglutination Technology For Doing Antibody Screening Test 3. Cell Panel Should Contain Rbc With The Antigens Of Extended Rh, Kell, Duffy, Kidd, Lewis, Mns Etc Blood Group System 4.Dose Depended Antigens ( Like Mns, Duffy Etc ) Should Be Present In Homozygous As Per Criteria Of Antibody Screening Test 5. Cell Panel Should Be Compatible For Enzyme Treatment 6. Pack Size Should Be3x10 Ml , Panel For Antibody 11 Cell Identification Test Applicable To Conventional Test Tube Method And Glass Bead Cassettes Technical Specification: 1. Cell Panel Should Be 2%-5% Of Rbc Suspended In Saline 2. Cell Panel Should Be Compatible With Both Conventional Test Tube Method And Glass Bead Cassettes Of Column Agglutination Technology For Doing Antibody Identification Test 3. Cell Panel Should Contain Rbc With The Antigens Of Extended Rh, Kell, Duffy, Kidd, Lewis, Mns Etc Blood Group System 4. Dose Depended Antigens ( Like Mns, Duffy Etc ) Should Be Present In Homozygous As Per Criteria Of Antibody Identification Test 5. Cell Panel Should Contain Rbc With The One Or More Heterozygous Expression Of Dose Dependent Antigens ( Mns, Duffy ) For Better Interpretation Of Result. 6. Cell Panel Should Be Compatible For Enzyme Treatment , Panel For Antibody 11 Cell Screening Applicable To Gel Card Technical Specification: 1. Cell Panel Should Be 0.8%-1%Of Rbc Suspended In Low Ionic Strength Saline 2. Cell Panel Should Be Compatible With Gel Cardof Column Agglutination Technology For Doing Antibody Screening Test 3.Cell Panel Should Contain Rbc With The Antigens Of Extended Rh, Kell, Duffy, Kidd, Lewis, Mns Etc Blood Group System 4. Dose Depended Antigens ( Like Mns, Duffy Etc ) Should Be Present In Homozygous As Per Criteria Of Antibody Identification Test 5. Cell Panel Should Contain Rbc With The One Or More Heterozygous Expression Of Dose Dependent Antigens ( Mns, Duffy ) For Better Interpretation Of Result. 6. Cell Panel Should Be Compatible With Enzyme Treatment , Low Ionic Strength Saline For Glass Bead Cassettes Low Ionic Strength Saline Should Be Compatible For Glass Bead Cassettes For Doing Antibody Screening , Antibody Identification Or Any Other Iat Test , Hiv ( Detection Of Both Ag & Ab ) Elisa Kit- 4Th Gen Technical Specification: 1. Kit Needs To Detect Antibodies Against Hiv 1+2 And P24 Ag Against Hiv-1. 2. Kit Needs To Be Based On Sandwich Elisa With Monoclonal Abs Against P24 Ag And Gp160 And Gp36 Recombinant Proteins On The Solid Phase 3. Kit Needs To Detect All The Three Classes Of Antibodies To Hiv I.E. Igm, Igg And Iga Simultaneously Providing Highest Early Sero-Conversion Sensitivity. 4. The Analytical Sensitivity Of P-24 Ag Detection Needs To Be = 25 Pg / Ml Or 1.0 Iu / Ml Of Who Standard. 5. Kit Sensitivity Needs To Be 100% 6. Kit Specificity Needs To Be = 99.5% For Both Antigen As Well As Antibody Without Compromising The Assay Sensitivity. 7. Kit Needs To Have Colour Coded Reagents With Od Norms For Reagents Addition Verification On Automation As Well As Manual Procedure. 8. Sample Volume Need To Be = 100 ?L Without Any Predilution Step. 9. Kits Needs To Have Reactive And Non-Reactive Controls With Separate Positive Control For Antigen And Antibody 10. Kit Needs To Be Ce Approved And Certificate Of Analysis Needs To Be Provided For Each Batch Of The Product 11. Total Incubation Needs To Be = 2 Hrs 12. Kit Needs To Be Programmable & Compatible For Automated System. 13. Supplier Needs To Provide The Certificate Of Analysis From Nib For Each Supplied Batch. 14. Supplier Needs To Be Able To Provide Antibody Confirmatory Kit For Further Analysis Of Reactive Cases On Demand 15. Strip Of Microplate Needs To Be Numbered And Microplate Frame Needs To Have Name Of The Assay For Easy Identification And Differentiation. 16. Reagents In The Kit Box Need To Have Barcode Label With Information Of Lot No And Expiry To Manage The Traceability In Automation , Hcv ( Detection Of Both Ag & Ab ) Elisa Kit - 4Th Gen Technical Specification: As Per Nit, Page No. - 24-25 , Hbsag Elisa Kit Technical Specification: As Per Nit, Page No. - 25-26
