Science and Technology Government Departments Closing in 0 days TDR #35628452

Tender For Supply Of Mouse Peroxiredoxin

Issued by Government Departments · mumbai, Maharashtra
Tender Value
Ref. Documents
Estimated cost
Bid Submission
23 Jan 2023
0 days left
EMD
Ref. Documents
Bank guarantee accepted
Document Fee
Ref. Documents
Non-refundable
Tender Type
Online

Tender Overview

Competition Type
NCB
Bidding Type
Tender
Location / State
mumbai → Maharashtra
EMD Exemption
Not Available
Quantity
Not Available

Project Description

Supply Of Mouse Peroxiredoxin Mouse Peroxiredoxin As Per Annexure Attached . , Mouse Peroxiredoxin 5 Lentiviral Activation Particles, Pack Of 200 Microlitres Of Transduction-Ready, High-Titer Crispr / Dcas9 Lentiviral Activation Particle, Based On Synergistic Activation Mediator ( Sam ) Transcription Activation System Consisting Ofdeactivated Cas9 ( Dcas9 ) Nuclease ( D10a And N863a ) Fused To The Transactivation Domain Vp64, An Ms2-P65-Hsf1 Fusion Protein And A Target-Specific 20 Nucleotide Guide Rna, Provides Robust Recruitment Of Transcription Factors For Highly Efficient Gene Activation Of Prdx5, Must Be Replication-Incompetent And Are Designed To Self-Inactivate After Transduction And Integration Into Genomic Dna Of Target Cells [ Vendor Should Provide Catalogue Number / Specification Sheet Of Product That Vendor Wishes To Supply To Allow Us To Cross Check Specifications ] , Mouse Peroxiredoxin 5 Double Nickase Plasmid, Pack Of 20 Micrograms, Suitable For Up To 20 Transfections, Consists Of A Pair Of Plasmids Each Encoding A D10a Mutated Cas9 Nuclease And A Target-Specific 20 Nt Guide Rna ( Grna ) Designed To Knockout Gene Expression With Greater Specificity Than Its Crispr / Cas9 Ko Counterpart, Paired Grna Sequences Are Offset By Approximately 20 Bp To Allow For Specific Cas9-Mediated Double Nicking Of The Genomic Dna, Which Mimics A Dsb; Antibiotic And Gfp Marker On Plasmids For Selection And Visual Confirmation [ Vendor Should Provide Catalogue Number / Specification Sheet Of Product That Vendor Wishes To Supply To Allow Us To Cross Check Specifications ] , Mouse Peroxiredoxin 5 Lentiviral Inactivation Particles, Pack Of 200 ?L Viral Stock Containing 1000000 Infectious Units Of Virus ( Ifu ) , Provided As Transduction-Ready Viral Particles For Gene Silencing, Specific For Mouse Prdx5, Contain Three To Five Expression Constructs Each Encoding Target-Specific 19-25 Nt ( Plus Hairpin ) Shrna Designed To Knockdown Gene Expression, Puromycin Or Similar Antibiotic Selection Marker, Replication-Incompetent And Are Designed To Self- Inactivate After Transduction And Integration Of Shrna Constructs Into Genomic Dna Of Target Cells [ Vendor Should Provide Catalogue Number / Specification Sheet Of Product That Vendor Wishes To Supply To Allow Us To Cross Check Specifications ] , 1, 5-Dimethyl-1, 5-Diazaundecamethylene Polymethobromide, Pack Of 1 Ml, Concentration Of 10 Mg / Ml, Infection Reagent Used To Introduce Retroviral Vectors Into Mammalian Cells, Meant To Be Used For Cell Culture By Manufacturer [ Vendor Should Provide Catalogue Number / Specification Sheet Of Product That Vendor Wishes To Supply To Allow Us To Cross Check Specifications ] , Negative Control Lentiviral Activation Particles, Pack Of 200 Microlites Of Transduction-Ready, High-Titer Crispr / Dcas9 Lentiviral Activation Particle, Based On Synergistic Activation Mediator ( Sam ) Transcription Activation System Consisting Ofdeactivated Cas9 ( Dcas9 ) Nuclease ( D10a And N863a ) Fused To The Transactivation Domain Vp64, An Ms2-P65-Hsf1 Fusion Protein And A Non-Specific 20 Nucleotide Guide Rna, Sam Complex Does Not Recognize Or Bind Any Specific Sequence Within The Genomic Dna, And Does Not Activate Transcription Of Any Specific Gene, Must Be Replication-Incompetent And Are Designed To Self-Inactivate After Transduction [ Vendor Should Provide Catalogue Number / Specification Sheet Of Product That Vendor Wishes To Supply To Allow Us To Cross Check Specifications ] , Control Lentiviral Particles With Gfp Coding Construct To Express In Mammalian Cells, Pack Of 200 Microlites Of Transduction-Ready Viral Particles Sufficient For 10 Or More Transductions, Stable Expression Of Gfp After Transduction, Meant To Measure Transduction Efficiency In Cell Type, Replication-Incompetent And Are Designed To Self-Inactivate After Transduction And Integration Of Gfp Control Constructs Into Genomic Dna Of Target Cells [ Vendor Should Provide Catalogue Number / Specification Sheet Of Product That Vendor Wishes To Supply To Allow Us To Cross Check Specifications ]

BOQ

Item Details: Lentiviral particles
Sl. No. Item Description
1Mouse Peroxiredoxin 5 lentiviral activation particles, pack of 200 microlitres of transduction-ready, high-titer CRISPR/dCas9 Lentiviral Activation Particle, based on synergistic activation mediator (SAM) transcription activation system consisting ofdeactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, an MS2-p65-HSF1 fusion protein and a target-specific 20 nucleotide guide RNA, provides robust recruitment of transcription factors for highly efficient gene activation of PRDX5, must be replication-incompetent and are designed to self-inactivate after transduction and integration into genomic DNA of target cells [vendor should provide catalogue number/specification sheet of product that vendor wishes to supply to allow us to cross check specifications]
2Mouse Peroxiredoxin 5 double nickase plasmid, pack of 20 micrograms, suitable for up to 20 transfections, consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart, paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB; antibiotic and GFP marker on plasmids for selection and visual confirmation [vendor should provide catalogue number/specification sheet of product that vendor wishes to supply to allow us to cross check specifications]
3Mouse Peroxiredoxin 5 lentiviral inactivation particles, pack of 200 µl viral stock containing 1000000 infectious units of virus (IFU), provided as transduction-ready viral particles for gene silencing, specific for mouse PRDX5, contain three to five expression constructs each encoding target-specific 19-25 nt (plus hairpin) shRNA designed to knockdown gene expression, puromycin or similar antibiotic selection marker, replication-incompetent and are designed to self-inactivate after transduction and integration of shRNA constructs into genomic DNA of target cells [vendor should provide catalogue number/specification sheet of product that vendor wishes to supply to allow us to cross check specifications]
41,5-Dimethyl-1,5-diazaundecamethylene polymethobromide, pack of 1 ml, concentration of 10 mg/ml, infection reagent used to introduce retroviral vectors into mammalian cells, meant to be used for cell culture by manufacturer [vendor should provide catalogue number/specification sheet of product that vendor wishes to supply to allow us to cross check specifications]
5Negative Control Lentiviral Activation Particles, pack of 200 microlites of transduction-ready, high-titer CRISPR/dCas9 Lentiviral Activation Particle, based on synergistic activation mediator (SAM) transcription activation system consisting ofdeactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, an MS2-p65-HSF1 fusion protein and a non-specific 20 nucleotide guide RNA, SAM complex does not recognize or bind any specific sequence within the genomic DNA, and does not activate transcription of any specific gene, must be replication-incompetent and are designed to self-inactivate after transduction [vendor should provide catalogue number/specification sheet of product that vendor wishes to supply to allow us to cross check specifications]
6Control Lentiviral Particles with GFP coding construct to express in mammalian cells, pack of 200 microlites of transduction-ready viral particles sufficient for 10 or more transductions,stable expression of GFP after transduction, meant to measure transduction efficiency in cell type, replication-incompetent and are designed to self-inactivate after transduction and integration of GFP Control constructs into genomic DNA of target cells [vendor should provide catalogue number/specification sheet of product that vendor wishes to supply to allow us to cross check specifications]

AI Tender Summary

OUR REF NO 35628452
AUTHORITY Government Departments
TENDER VALUE Ref. Documents
LAST DATE 23-01-2023
Authority
Authority Name Directorate Of Purchase And Stores
Work Description Supply Of Mouse Peroxiredoxin Mouse Peroxiredoxin As Per Annexure Attached . , Mouse Peroxiredoxin 5 Lentiviral Activation Particles, Pack Of 200 Microlitres Of Transduction-Ready, High-Titer Crispr / Dcas9 Lentiviral Activation Particle, Based On Synergistic Activation Mediator ( Sam ) Transcription Activation System Consisting Ofdeactivated Cas9 ( Dcas9 ) Nuclease ( D10a And N863a ) Fused To The Transactivation Domain Vp64, An Ms2-P65-Hsf1 Fusion Protein And A Target-Specific 20 Nucleotide Guide Rna, Provides Robust Recruitment Of Transcription Factors For Highly Efficient Gene Activation Of Prdx5, Must Be Replication-Incompetent And Are Designed To Self-Inactivate After Transduction And Integration Into Genomic Dna Of Target Cells [ Vendor Should Provide Catalogue Number / Specification Sheet Of Product That Vendor Wishes To Supply To Allow Us To Cross Check Specifications ] , Mouse Peroxiredoxin 5 Double Nickase Plasmid, Pack Of 20 Micrograms, Suitable For Up To 20 Transfections, Consists Of A Pair Of Plasmids Each Encoding A D10a Mutated Cas9 Nuclease And A Target-Specific 20 Nt Guide Rna ( Grna ) Designed To Knockout Gene Expression With Greater Specificity Than Its Crispr / Cas9 Ko Counterpart, Paired Grna Sequences Are Offset By Approximately 20 Bp To Allow For Specific Cas9-Mediated Double Nicking Of The Genomic Dna, Which Mimics A Dsb; Antibiotic And Gfp Marker On Plasmids For Selection And Visual Confirmation [ Vendor Should Provide Catalogue Number / Specification Sheet Of Product That Vendor Wishes To Supply To Allow Us To Cross Check Specifications ] , Mouse Peroxiredoxin 5 Lentiviral Inactivation Particles, Pack Of 200 ?L Viral Stock Containing 1000000 Infectious Units Of Virus ( Ifu ) , Provided As Transduction-Ready Viral Particles For Gene Silencing, Specific For Mouse Prdx5, Contain Three To Five Expression Constructs Each Encoding Target-Specific 19-25 Nt ( Plus Hairpin ) Shrna Designed To Knockdown Gene Expression, Puromycin Or Similar Antibiotic Selection Marker, Replication-Incompetent And Are Designed To Self- Inactivate After Transduction And Integration Of Shrna Constructs Into Genomic Dna Of Target Cells [ Vendor Should Provide Catalogue Number / Specification Sheet Of Product That Vendor Wishes To Supply To Allow Us To Cross Check Specifications ] , 1, 5-Dimethyl-1, 5-Diazaundecamethylene Polymethobromide, Pack Of 1 Ml, Concentration Of 10 Mg / Ml, Infection Reagent Used To Introduce Retroviral Vectors Into Mammalian Cells, Meant To Be Used For Cell Culture By Manufacturer [ Vendor Should Provide Catalogue Number / Specification Sheet Of Product That Vendor Wishes To Supply To Allow Us To Cross Check Specifications ] , Negative Control Lentiviral Activation Particles, Pack Of 200 Microlites Of Transduction-Ready, High-Titer Crispr / Dcas9 Lentiviral Activation Particle, Based On Synergistic Activation Mediator ( Sam ) Transcription Activation System Consisting Ofdeactivated Cas9 ( Dcas9 ) Nuclease ( D10a And N863a ) Fused To The Transactivation Domain Vp64, An Ms2-P65-Hsf1 Fusion Protein And A Non-Specific 20 Nucleotide Guide Rna, Sam Complex Does Not Recognize Or Bind Any Specific Sequence Within The Genomic Dna, And Does Not Activate Transcription Of Any Specific Gene, Must Be Replication-Incompetent And Are Designed To Self-Inactivate After Transduction [ Vendor Should Provide Catalogue Number / Specification Sheet Of Product That Vendor Wishes To Supply To Allow Us To Cross Check Specifications ] , Control Lentiviral Particles With Gfp Coding Construct To Express In Mammalian Cells, Pack Of 200 Microlites Of Transduction-Ready Viral Particles Sufficient For 10 Or More Transductions, Stable Expression Of Gfp After Transduction, Meant To Measure Transduction Efficiency In Cell Type, Replication-Incompetent And Are Designed To Self-Inactivate After Transduction And Integration Of Gfp Control Constructs Into Genomic Dna Of Target Cells [ Vendor Should Provide Catalogue Number / Specification Sheet Of Product That Vendor Wishes To Supply To Allow Us To Cross Check Specifications ]
Basic Detail
Tender No DPS/CPU/04/A3/3889
Bidding Type Tender
Location
City mumbai
State Maharashtra
Key Dates
Publish Date 06 Jan 2023
Submission Date 23 Jan 2023
Open Date 24 Jan 2023
Finance
Tender Value Ref. Documents
Tender Fee Ref. Documents
EMD Ref. Documents
Exemption Not Available
Document List
68b4ec39-ce50-4c39-a63f-bfa7388cd30f.html
ANNEX.pdf
BOQ_770493.xls
DECLARATION-FORMS.rar
NIT_CHECKLIST.pdf
NIT_COMMERCIAL_TERMS.pdf
Note-to-Vendors.pdf
Tendernotice_1.pdf

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Tender Timeline

Jan 06, 2023
11:30 IST

Tender Published

Tender notice published.

Completed
Jan 23, 2023
17:00 IST

Bid Submission Deadline

Online submission via eProcurement portal.

Completed
Jan 24, 2023

Bid Opening Date

Technical bids will be opened and evaluated.

Completed

Tender Documents

Download All (ZIP) ↓
html

68b4ec39-ce50-4c39-a63f-bfa7388cd30f.html

pdf

ANNEX.pdf

xls

BOQ_770493.xls

rar

DECLARATION-FORMS.rar

pdf

NIT_CHECKLIST.pdf

pdf

NIT_COMMERCIAL_TERMS.pdf

pdf

Note-to-Vendors.pdf

pdf

Tendernotice_1.pdf

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