Delhi University has published Tender For Supply, Installation, Testing And Commissioning Of Real Time Pcr System, Refrigerated Micro Centrifuge, Microplate Centrifuge And Minus 20 Deg Deep Freezer Scientific Equipment For Hansraj College, University Of Delhi. Submission Date for this Tender is 19-01-2023. Freezer Tenders in Delhi Delhi. Bidders can get complete Tender details and download the document.
| Sr No | CorrigendumDate | Corrignedum | CorrigendumType | NewSubmissionDate |
| 1 | 12-Jan-2023 | Extension of Date | Date | 19-01-2023 |
| Sl. No. | Item Description |
| 1 | Real Time PCR (Details can be checked through tender document) |
| 2 | 1. System should run real-time PCR experiments without being attached to a computer. When operated as a stand-alone instrument, the instrument will save at least 1000 run files. 2. Real-time data traces can be viewed during a run from the thermal cycler screen. 3. System includes an automated lid that opens, closes, and can apply sealing force to reaction wells. 4. System must not have lid or drawer that extends beyond the footprint of the system nor requires additional operating clearance. 5. System can detect a 1.3-fold change in gene expression. 6. Thermal gradient for optimization of multiple temperatures in a single assay. Temperature differences of up to 24°C front-to-back can be created. 7. Peltier-driven thermal cycler with maximum ramping speed of 5°C/sec, with an average ramp rate of 3.3°C/sec. 8. Should have a thermal block operational range of 4–100°C. 9. Sample block temperature accuracy is +/-0.2°C of programmed target at 90°C, with a uniformity of +/-0.3°C well-to-well within 10 seconds of arrival at 90°C. 10. Optical system allows excitation and detection of up to five fluorescent dyes in a single reaction well. 11. Optics independently illuminates and detects fluorescence from each well with the same LED/detector pair per channel. The system should have six filtered LEDs for illumination and differentially detects emission using six filtered photodiodes (one for each channel plus FRET). 12. System must have fixed optical path, directly over each well, eliminates the need to normalize for positional bias. 13. Absorption spectra in the 450–684 nm; Emission spectra in the 515–730 nm range. 14. One channel is dedicated for FRET and Protein Thermal Shift (Protein Melt) experiments. 15. System should reads all 96 wells with all channels within 12 seconds. 16. In “SYBR/FAM” scan mode, the system should read all 96 wells within 3 seconds. 17. Dynamic range of 10 orders of magnitude. 18. Should detects one copy of target sequence in human genomic DNA. 19. Reaction volumes from 1–50 µl. 20. Should detects =10 fmol of fluorescein. 21. System can be integrated with an automation system for hands-free operation. 22. System should continue to run and complete a run if the software is stopped or interrupted to prevent run data from being list in case if there is unintentional interruption of the software. This function is especially valuable for precious or limited samples, whereby all data is not lost for a run that was interrupted. 23. System should directly connect to a network file storage location through Wi-Fi or ethernet with user credentials and can read and write, given system access rights. 24. System should connect to cloud platform for remote monitoring, experiment set up, and Cq analysis. 25. The bidder must be 100 or more installation in Delhi NCR of the quoted model. User list must be attached. 1. Should be supplied with compatible UPS with 30 min back up for better performance of the instrument. 2. Must be supplied with compatible Desktop. • System should be quoted with three years warranty. 1. Multiplex amplification and melt curve and end-point analyses should be performed on up to five fluorophores in a single reaction well. 2. Multiple plates should be combined into one experiment with the Gene Study Feature. 3. Run results should be displayed in a customizable configuration so that multiple panes of information can be viewed in a single window. 4. Multiple standard curves should be simultaneously viewed when a common fluorophore is used for multiple targets. 5. PCR quantification by standard curve features automated calculations of reaction efficiencies with y-intercept. 6. Automated allelic discrimination by end-point fluorescence or quantification cycle (Cq) values. 7. Gene expression analysis by relative quantity (?Cq) or normalized expression (??Cq). 8. Multiple reference genes Could be assigned in normalized expression (??Cq) analysis. 9. Software should have reference Gene Selector Tool displays gene stability for selection of ideal reference genes. 10. Up to 5,000 Cq values from different data files should be compared for gene expression analysis. 11. Embedded reports tool should be customizable by the user to export run information, data tables, graphs, and analysis parameters in a specified order to PDF or other file formats. 12. Software should be laboratory information management system (LIMS) enabled. 13. Software should be able to data to be grouped and interpret by both technical replicates and biological groups. 14. Image export options must include DPI selection up too 600DPI, choice of any image size, and color selection using RGB specifications. 15. Image annotation function allow for automatic p-value annotation and addition of arrows, circles, and text directly onto graph images. 16. Software should perform t-tests and 1-way ANOVA calculations. 17. Software should display data in multiple formats including bar chart, dot plot, box-and-whisker plot, scatter plot, cluster gram, and volcano plot. 18. Should control up to 4 Instruments with one PC. |
| 3 | Refrigerated Microcentrifuge: (Details can be checked through tender document) |
| 4 | • Maximum RCF must be 17,000xg or above • Maximum Speed must be 13,000 RPM or above • Should be supplied with Rotor 24x1.5ml/2ml with Click seal biocontainment lid approved by HPA, Porton Down, UK for Biocontainment. • Large LED display for Time, Speed and Temperature • Max Noise Level should be 50 dBA • Temperature set range from minus 9 °C to plus 40°C • Accelaration/Deccelaration time 10Sec/12 Sec • Time set range 1 to 99 min, 1 min increments • Toggle between RPM and RCF. • Induction maintenance free motor. • System must be CSA certified, CE marked, IVD compliant, Certified biocontainment • System should have wide selection of following rotors for future upgrade - Dual Row rotor 18x2 plus 18x0.5ml for simultaneous run of two different volumes without using adapters. - 36x0.5ml rotor - PCR 4x8 (32x0.2ml) rotor with Click seal Biocontainment lid approved by HPA, Porton Down, UK for Biocontainment. - PCR 8x8(64x0.2ml) rotor - Must be 50 or more installation of the quoted model. User list must be attached. • System should be quoted with two years warranty. |
| 5 | Microplate Centrifuge (Details can be checked through tender document) |
| 6 | 1. System should have Brushless DC motor for maintenance free long life. 2. System must have the capacity of 2 microplates :96 & 384 well (PCR/ Deep well) & compatible with all types of PCR plates. 3. System must have Microprocessor controlled centrifuge with variable speed and time setting 1-999 min & infinity mode along with last run memory function & we can save and run upto 99 defined programs (protocols) 4. System must have minimum Precise RPM setting from 500 to 2200 RPM or above with an accuracy of ±10 RPM 5. System dimensions should not more than LXWXH (mm) 415 x 355 x 173 6. System must have automatic imbalance detection for safe operation 7. System must have features of Lid lock safety (Automatic lid release after run completion) 8. System must have digital display. 9. Compact design with low loading height for ease of operation 10. System must have minimum one year warranty. 11. System must have one touch short spin operation & change over from RPM to RCF |
| 7 | Minus 20 Deg C (Details can be checked through tender document) |
| 8 | 1. Temperature Range should be -17 to -24 degree C. 2. Capacity should be 140-150 Ltr 3. System dimensions should not more than WXDXH(INCH)21 X 22 X 45 4. System must have an option of manual defrost and rated load should be 120-130W 5. System should have 5 pull out drawers. 6. System should have solid door 7. REFRIGERENT should be R134a 8. System should have visual & Acoustic Alarm 9. System should be CE certified 10. System should have Super-Freeze Function & reversible door. 11. system should have an option for good lightning and transparent drawers and flaps maximise the visibility the product 12. Warranty should be standard as per principal norms. 13. Must be supplied with compatible stablizer for better performance of the instrument. |
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