Tender For Rate Contract For Supply Of Consumables And Non Consumables, Pondicherry-Puducherry

Rate Contract For Supply Of Consumables And Non Consumables Rate Contract For Supply Of Consumables And Non Consumables For The Virology Section, Dept. Of Microbiology , Consumables , Probe Pcr Mastermix Universal Colored Qpcr Master Mix Compatible With Taqman Probes At 2X Concentration Included With Udg Enzyme In The Master Mix To Prevent Carry-Over Contamination, Hot Start Taq Dna Polymerase. Nuclease Free Water And Necessary Sample Buffers Should Be Provided. Udg Enzyme May Also Be Given As Separate Vial , One Step Rt-Pcr Mastermix One Step Reverse Transcription Real Time Pcr Master Mix Compatible With Taqman Probes With Heat Labile Udg Enzyme In The Master Mix To Prevent Carry-Over Contamination, Rt Enzyme, Hot Start Taq Dna Polymerase. Nuclease Free Water And Necessary Sample Buffers Should Be Provided. Udg Enzyme May Also Be Given As Separate Vial , Cdna Synthesis Kit First Strand Cdna Synthesis Kit Containing Reverse Transcriptase, Rnase Inhibitor, Dntp Mix, Oligo Dt Primer, Random Hexamer Primer, And Buffer. , Hotstart Conventional Pcr Mastermix Conventional Hot Start Pcr Master Mix, Should Be Ready To Load Directly From Pcr Tube To Gel , Pcr Product Gel Purification Kit ( Spin Column ) Spin Column-Based Pcr Gel Purification Kit With Necessary Buffers , Pcr Product Purification Kit ( Spin Column ) Spin Column-Based Direct Pcr Product Purification Kit With Necessary Buffers , Molecular Grade Agarose For Gel Electrophoresis 500G Pack , Tris Borate Edta Buffer ( 10X ) For Gel Electrophoresis , Tris Edta Buffer ( 1X ) , Rnase, Dnase Free Molecular Grade 100 Ml , Nucleic Acid Extraction Plasticware Compatible For Thermo Kingfisher Flex ( 4 Nos Of 96 Position -Deep Well Plates, 1 Comb Plate, 1 Comb , Nucleic Acid Extraction Plasticware Compatible For Genetix Automated Nucleic Acid Extraction Machine ( 4 Nos Of 96 Position -Deep Well Plates, 1 Comb Plate, 1 Comb , Probe 5 Hex + 3 Bhq-1™ 10 Nmol , Probe 5 Hex + 3 Bhq-1™ 25 Nmol , Probe 5 Hex + 3 Bhq-1™ 40 Nmol , Probe 5 Tamara + 3 Bhq-2™ 10 Nmol , Probe 5 Tamara + 3 Bhq-2™ 25 Nmol , Probe 5 Tamara + 3 Bhq-2™ 40 Nmol , Probe 5 Rox + 3 Bhq-2™ 10 Nmol , Probe 5 Rox + 3 Bhq-2™ 25 Nmol , Probe 5 Rox + 3 Bhq-2™ 40 Nmol , Probe 5 Cy5® + 3 Bhq-2™ 10 Nmol , Probe 5 Cy5® + 3 Bhq-2™ 25 Nmol , Probe 5 Cy5® + 3 Bhq-2™ 40 Nmol , Mgb Probe 5 6-Fam-Labelled 6 Nmol , Mgb Probe 5 6-Fam-Labelled 10 Nmol , Mgb Probe 5 Texas Red-Labelled 6 Nmol , Mgb Probe 5 Texas Red-Labelled 10 Nmol , Mgb Probe 5 Hex-Labelled 6 Nmol , Mgb Probe 5 Hex-Labelled 10 Nmol , Dna Base / Per Base 10 Nmol Primer Synthesis , Dna Base / Per Base 25 Nmol Primer Synthesis , 3 Wobble ( Degenerate Bases ) 40 Nmol , Mgb Probe 5 Yakima Yellow® / Vic 6 Nmol , Mgb Probe 5 Yakima Yellow® / Vic 10 Nmol , Mgb Probe 5 Dragonfly 6 Nmol , Mgb Probe 5 Dragonfly 10 Nmol , Sanger Sequencing Services Sequencing Service For 1 Sample Includes Pcr Product Purification, Both Forward And Reverse Strand Sequencing , Sterile Cell Culture Pipette ( 5 Ml ) Sterile, Rnase- / Dnase-Free, Nonpyrogenic , Noncytotoxic , Exclusive Anti-Drip Tip Assures Accurate Delivery. Ideal For Sterile Tissue Culture Applications. Color-Coded Magnifier Stripes Make Volume Reading And Size Selection Easier. Bidirectional Graduations Provide Choice Of Ascending And Descending Scales. Negative Graduations Allow Additional Working Volume. Packaging Options: - Individually Wrapped, Paper / Plastic - Individually Wrapped, Clear Plastic Volumetric Accuracy Of ±2% At Stated Capacity , Sterile Cell Culture Pipette ( 10 Ml ) Sterile, Rnase- / Dnase-Free, Nonpyrogenic , Noncytotoxic , Exclusive Anti-Drip Tip Assures Accurate Delivery. Ideal For Sterile Tissue Culture Applications. Color-Coded Magnifier Stripes Make Volume Reading And Size Selection Easier. Bidirectional Graduations Provide Choice Of Ascending And Descending Scales. Negative Graduations Allow Additional Working Volume. Packaging Options: - Individually Wrapped, Paper / Plastic - Individually Wrapped, Clear Plastic Volumetric Accuracy Of ±2% At Stated Capacity , Cell Culture Medium Dmem Powdered Media Of Dulbeccos Modified Eagle Medium ( Dmem ) With 4.5Gms Glucose Per Litre And L-Glutamine Without Sodium Bicarbonate And Sodium Pyruvate. Ready To Reconsitute In 1 Liter Of Distilled Water. , Antibiotic, Antimycotic Solution ( 20 Ml Pack ) Antibiotic Antimycotic Solution 100X Liquid W / 10, 000 U Penicillin, 10Mg Streptomycin And 25 ?G Amphotericin B Per Ml In 0.9% Normal Saline Sterile Filtered, Sutable For Cell Culture. Exdotoxin Tested. , 6-Well Flat-Bottom Plate 6-Well Flat-Bottom Plate, Tissue Culture- Clear Polystyrene Flat-Bottom, Tissue Culture-Treated Multiwell Plate With Lid. Non-Pyrogenic, Sterile, Rnase- / Dnase-Free. ( Individually Wrapped ) , 12 Well Plate Flat-Bottom Plate. 12 Well Plate, Flat Bottom, Polystyrene, Sterile, With Lid. Non-Pyrogenic, Sterile, Rnase- / Dnase-Free. ( Individually Wrapped ) , T-25 Flask Flask: Optically Clear Polystyrene Cap: Non Vented High-Density Polyethylene Growth Area Is Tissue Culture-Treated For Optimal Cell Attachment Non-Pyrogenic, Sterile, Rnase- / Dnase-Free, Neck Style: Angled. Approximate Cell Growth Area Of 25 Cm². Recommended Working Volume Of 5-7 Ml. Sterility By Gamma Irradiation , T-75 Flask Flask: Optically Clear Polystyrene Cap: Non Vented High-Density Polyethylene Growth Area Is Tissue Culture-Treated For Optimal Cell Attachment Non-Pyrogenic, Sterile, Rnase- / Dnase-Free, Neck Style: Angled. Approximate Cell Growth Area Of 75 Cm². Recommended Working Volume Of 15 - 22.5 Ml. Sterility By Gamma Irradiation , Trypsin-Edta ( 0.25% ) , With Phenol Red ( 100 Ml Pack ) Liquid Formulation Of Trypsin Contains Edta And Phenol Red. Trypsin Solutions Are Tested For Ph, Osmolality, Sterility, And Performance. In Addition, The Raw Materials Are Verified For E-Beam Irradiation And Tested For Endotoxin, Ppv, Pcv 1 / 2, Mycoplasma, Bacterial, Fungal, And Viral Contamination. , Trypsin, Tpck Treated ( 25 Ug / Vial ) Form-Powder, Cell Culture Grade. , V-Bottom Micro Titre Plate V-Bottom Clear Wells, Polystyrene, Sterile, Nonpyrogenic , Noncytotoxic, With Lid, Packed Individually. , Conical Tubes ( 50 Ml ) Sterile Non- Skirted, Hydrophobic, , Leak-Proof , Biologically Inert Surface For Good Cell Or Protein Recovery, Graduations In 5Ml Increments, White Writing Area Resists Solvents, Irradiated For Sterility, Resistant To Alcohols And Mild Organic Solvents, Translucent Wall For Easy Viewing. Stable From -80°C To +121°C ( -112°F To +250°F ) , Withstand Centrifugal Forces To 16, 000 X G Rcf. , Conical Tubes ( 15 Ml ) Sterile Hydrophobic, , Leak-Proof , Biologically Inert Surface For Good Cell Or Protein Recovery, Graduations In 5Ml Increments, White Writing Area Resists Solvents, Irradiated For Sterility, Resistant To Alcohols And Mild Organic Solvents, Translucent Wall For Easy Viewing. Stable From -80°C To +121°C ( -112°F To +250°F ) , Withstand Centrifugal Forces To 16, 000 X G Rcf. , Propidium Iodide ( 1 Mg / Ml ) Solution 10 Ml Pack Purity: =94%, Application: Cell Staining For Flow Cytometry , Goat Anti-Rabbit Igg H&L ( Alexa Fluor® 647 ) 500 Ug Pack Application: Ihc, Flowcytometry, Fat , Cell Freezing Medium-Dmso, 1X Without Antibiotics Sterile Filtered, 50 Ml Pack For Cell Preservation In Liquid Nitrogen , 1000-Ul Conductive Filter Tips Colour: Black Compatability For: Tecan Freedom Evo 100 Liquid Handler Type: Filter Tip, Conductive For Liquid Sensing, Sterile Pipette Compatibility For: Tecan Airliha , 200-Ul Conductive Filter Tips Colour: Black Compatability For: Tecan Freedom Evo 100 Liquid Handler Type: Filter Tip, Conductive For Liquid Sensing, Sterile Pipette Compatibility For: Tecan Airliha

INR 590 /-

INR 5000.0 /-

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Name of Work: RATE CONTRACT for supply of CONSUMABLES/NON-CONSUMABLES for the Virology Section, Dept. of Microbiology, JIPMER, Puducherry – 605 006

Sl. No.	Item Description
1	CONSUMABLES
2	Probe PCR mastermix Universal colored qPCR master mix compatible with TaqMan probes at 2X concentration included with UDG enzyme in the master mix to prevent carry-over contamination, hot Start Taq DNA Polymerase. Nuclease free water and necessary sample buffers should be provided. UDG enzyme may also be given as separate vial
3	One step RT-PCR mastermix One step Reverse Transcription real time PCR master mix compatible with TaqMan probes with heat labile UDG enzyme in the master mix to prevent carry-over contamination, RT enzyme, hot Start Taq DNA Polymerase. Nuclease free water and necessary sample buffers should be provided. UDG enzyme may also be given as separate vial
4	cDNA synthesis kit First strand cDNA synthesis kit containing Reverse Transcriptase, RNase Inhibitor, dNTP mix, Oligo dT primer, random hexamer primer, and buffer.
5	Hotstart conventional PCR mastermix Conventional hot start PCR master mix, should be ready to load directly from PCR tube to gel
6	PCR product gel purification kit (spin column) Spin column-based PCR gel purification kit with necessary buffers
7	PCR product purification kit (spin column) Spin column-based direct PCR product purification kit with necessary buffers
8	Molecular grade Agarose for gel electrophoresis 500g pack
9	Tris Borate EDTA buffer (10x) for gel electrophoresis
10	Tris EDTA buffer (1x), RNAse, DNase free molecular grade 100 ml
11	Nucleic acid Extraction plasticware compatible for Thermo KingFisher Flex (4 nos of 96 position -deep well plates, 1 comb plate, 1 comb
12	Nucleic acid Extraction plasticware compatible for Genetix automated nucleic acid extraction machine (4 nos of 96 position -deep well plates, 1 comb plate, 1 comb
13	Probe 5" HEX + 3" BHQ-1™ 10 nmol
14	Probe 5" HEX + 3" BHQ-1™ 25 nmol
15	Probe 5" HEX + 3" BHQ-1™ 40 nmol
16	Probe 5" TAMARA + 3" BHQ-2™ 10 nmol
17	Probe 5" TAMARA + 3" BHQ-2™ 25 nmol
18	Probe 5" TAMARA + 3" BHQ-2™ 40 nmol
19	Probe 5" ROX + 3" BHQ-2™ 10 nmol
20	Probe 5" ROX + 3" BHQ-2™ 25 nmol
21	Probe 5" ROX + 3" BHQ-2™ 40 nmol
22	Probe 5" Cy5® + 3" BHQ-2™ 10 nmol
23	Probe 5" Cy5® + 3" BHQ-2™ 25 nmol
24	Probe 5" Cy5® + 3" BHQ-2™ 40 nmol
25	MGB Probe 5" 6-FAM-labelled 6 nmol
26	MGB Probe 5" 6-FAM-labelled 10 nmol
27	MGB Probe 5" Texas Red-labelled 6 nmol
28	MGB Probe 5" Texas Red-labelled 10 nmol
29	MGB Probe 5" HEX-labelled 6 nmol
30	MGB Probe 5" HEX-labelled 10 nmol
31	DNA Base/ Per Base 10 nmol primer synthesis
32	DNA Base/ Per Base 25 nmol primer synthesis
33	3" Wobble (Degenerate bases) 40 nmol
34	MGB probe 5" Yakima Yellow® /VIC 6 nmol
35	MGB probe 5" Yakima Yellow®/VIC 10 nmol
36	MGB probe 5" DragonFly 6 nmol
37	MGB probe 5" DragonFly 10 nmol
38	Sanger sequencing services Sequencing service for 1 sample includes PCR product purification, both forward and reverse strand sequencing
39	Sterile cell culture pipette (5 ml) Sterile, RNase-/DNase-free, Nonpyrogenic , Noncytotoxic , Exclusive anti-drip tip assures accurate delivery. Ideal for sterile tissue culture applications. Color-coded magnifier stripes make volume reading and size selection easier. Bidirectional graduations provide choice of ascending and descending scales. Negative graduations allow additional working volume. Packaging options: - Individually wrapped, paper/plastic - Individually wrapped, clear plastic Volumetric accuracy of ±2% at stated capacity
40	Sterile cell culture pipette (10 ml) Sterile, RNase-/DNase-free, Nonpyrogenic , Noncytotoxic , Exclusive anti-drip tip assures accurate delivery. Ideal for sterile tissue culture applications. Color-coded magnifier stripes make volume reading and size selection easier. Bidirectional graduations provide choice of ascending and descending scales. Negative graduations allow additional working volume. Packaging options: - Individually wrapped, paper/plastic - Individually wrapped, clear plastic Volumetric accuracy of ±2% at stated capacity
41	Cell culture medium DMEM Powdered media of Dulbecco"s Modified Eagle Medium (DMEM) With 4.5gms Glucose per litre and L-Glutamine Without Sodium bicarbonate and Sodium pyruvate. Ready to reconsitute in 1 liter of distilled water.
42	Antibiotic, antimycotic solution (20 ml pack) Antibiotic Antimycotic Solution 100X liquid w/10,000 U Penicillin, 10mg Streptomycin and 25 µg Amphotericin B per ml in 0.9% normal saline Sterile filtered, sutable for cell culture. Exdotoxin tested.
43	6-Well Flat-Bottom Plate 6-Well Flat-Bottom Plate, Tissue Culture- Clear polystyrene flat-bottom, tissue culture-treated multiwell plate with lid. Non-pyrogenic, Sterile, RNase-/DNase-free. (individually wrapped)
44	12 Well plate Flat-bottom plate. 12 well plate, flat bottom, polystyrene, sterile, with lid. Non-pyrogenic, Sterile, RNase-/DNase-free. (individually wrapped)
45	T-25 flask Flask: optically clear polystyrene Cap: non vented high-density polyethylene Growth area is tissue culture-treated for optimal cell attachment Non-pyrogenic, Sterile, RNase-/DNase-free, Neck Style: angled. Approximate cell growth area of 25 cm². Recommended working volume of 5-7 mL. Sterility by Gamma irradiation
46	T-75 flask Flask: optically clear polystyrene Cap: non vented high-density polyethylene Growth area is tissue culture-treated for optimal cell attachment Non-pyrogenic, Sterile, RNase-/DNase-free, Neck Style: angled. Approximate cell growth area of 75 cm². Recommended working volume of 15 - 22.5 mL. Sterility by Gamma irradiation
47	Trypsin-EDTA (0.25%), with phenol red (100 ml pack) Liquid formulation of trypsin contains EDTA and phenol red. Trypsin solutions are tested for pH, osmolality, sterility, and performance. In addition, the raw materials are verified for e-beam irradiation and tested for endotoxin, PPV, PCV 1/2, mycoplasma, bacterial, fungal, and viral contamination.
48	Trypsin, TPCK Treated (25 ug/vial) Form-Powder, cell culture grade.
49	V-bottom micro titre plate V-bottom clear wells, polystyrene, sterile, Nonpyrogenic , Noncytotoxic, with lid, packed individually.
50	Conical Tubes (50 ml ) sterile Non- skirted, Hydrophobic, ,Leak-proof , biologically inert surface for good cell or protein recovery, graduations in 5mL increments, White writing area resists solvents, Irradiated for sterility, Resistant to alcohols and mild organic solvents, translucent wall for easy viewing. Stable from -80°C to +121°C (-112°F to +250°F), Withstand centrifugal forces to 16,000 x g RCF.
51	Conical Tubes (15 ml ) sterile Hydrophobic, ,Leak-proof , biologically inert surface for good cell or protein recovery, graduations in 5mL increments, White writing area resists solvents, Irradiated for sterility, Resistant to alcohols and mild organic solvents, translucent wall for easy viewing. Stable from -80°C to +121°C (-112°F to +250°F), Withstand centrifugal forces to 16,000 x g RCF.
52	Propidium iodide (1 mg/ml) solution 10 ml pack Purity: =94%, Application: cell staining for flow cytometry
53	Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) 500 ug pack Application: IHC, flowcytometry, FAT
54	Cell Freezing Medium-DMSO, 1X Without Antibiotics Sterile filtered, 50 ml pack For Cell preservation in liquid nitrogen
55	1000-uL conductive filter tips Colour: Black Compatability for: Tecan Freedom Evo 100 liquid handler Type: Filter tip, conductive for liquid sensing, sterile Pipette compatibility for: Tecan AirLiHa
56	200-uL conductive filter tips Colour: Black Compatability for: Tecan Freedom Evo 100 liquid handler Type: Filter tip, conductive for liquid sensing, sterile Pipette compatibility for: Tecan AirLiHa