Procurement Of Laboratory Consumables Procurement Of Laboratory Consumables , Thermo Fisher Manufactured, Commissioned Reagents And Kits , Trypsin-Edta (0.5%), No Phenol Red , Tryple™ Express Enzyme (1X), No Phenol Red , Neon™ Transfection System 100 ?L K, burdwan-West Bengal

Procurement Of Laboratory Consumables Procurement Of Laboratory Consumables , Thermo Fisher Manufactured, Commissioned Reagents And Kits , Trypsin-Edta (0.5%), No Phenol Red , Tryple™ Express Enzyme (1X), No Phenol Red , Neon™ Transfection System 100 ?L Kit , Neon™ Transfection System 10 ?L Kit , Lipofectamine™ Crisprmax™ Cas9 Transfection Reagent , Pes Syringe Filters , Pes Syringe Filters , Truecut™ Cas9 Protein V2 , Geneart™ Genomic Cleavage Detection Kit , Cd27 Monoclonal Antibody (Lg.7F9) , Alexa Fluor™ 594 Goat Anti-Rat Igg , Alexa Fluor™ 647 Anti-Mouse Igg , Purelink™ Hipure Plasmid Midiprep Kit , Opti-Mem™ , Generic , Protein Electrophoresis System , Wet/Tank Transfer System , Novolink™ Polymer Detection Systems , Rneasy Plus Mini Kit (50) , Biolegend Item , Apc Anti-Human Cd11c , Fitc Anti-Human Hla-Dr , Pe Anti-Human Cd80 , Pe/Cyanine7 Anti-Human Cd86 , Apc/Cyanine7 Anti-Human Cd14 , Alexa Fluor® 700 Anti-Human Cd40 , Brilliant Violet 421™ Anti-Human Cd197 (Ccr7) , Purified Anti-Human Cd45 Antibody , 7-Aad Viability Staining Solution , Anti-Mouse Cd19 Antibody , Anti-Mouse/Human Cd207 , Purified Anti-Mouse Cd20 Antibody , Brilliant Violet 421 Anti-Rat Igg , Himedia Laboratories , Rpmi 1640 , Rpmi 1640 , Antibiotic Solution 100× , Fbs , Ezkill™ Mycoplasma Elimination Kit , Carbinol Lr , Hi-Quanti One Step Sybr Based Rt-Pcr Kit , Hiscript One Step Rt-Pcr Kit (Semi-Q Pcr Based) , 5X Protein Loading Buffer (Reducing) , Acrylamide , Trasons Item , Tissue Culture Petri Dish 35 Mm , Tissue Culture Petri Dish 60 Mm , Microtips 0.2-10 ?L , Microtips 2-200 ?L , Microtips 200-1000 ?L , Microcentrifuge Tube 1.5 Ml , Cryovial 1.8 Ml , Corning , Corning 6-Well Plate , Corning 12 Well Plate , Corning 24 Well Plate , Corning Cell Scrapers , Corning Serological Pipets5 Ml , Corning Serological Pipettes 10 Ml , Corning Serological Pipettes 25 Ml , Corning® 25Cm² Cell Culture Flask With Vent Cap , Corning® 75 Cm² U-Shape Cell Culture Flask, Canted Neck , Cell Signaling Technology , Apoptosis Antibody Sampler Kit , Calreticulin (D3e6) Xp® Rabbit Mab , Ctla-4 (E2v1z) Rabbit Mab , Jurkat Apoptosis Cell Extracts , Cell Fractionation Kit , Merck-Millipore Manufactured, Commissioned Reagents And Kits , Sodium Azide , Dpx Mountant For Histology , Paraplast Plus , Tipiracil Hydrochloride , Ficoll®-Paque Premium , Xylenes , Human Serum , Predesigned Crispr Grna With Positive Control

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Name of Work: Supply of Chemicals, Reagents, Kits, Plasticware etc for the DST-SERB Project (PI-Dr. Sumit Kr. Hira)

Sl. No.	Item Description
1	Thermo Fisher Manufactured, commissioned Reagents and Kits
2	Trypsin-EDTA (0.5%), no phenol red
3	TrypLE™ Express Enzyme (1X), no phenol red
4	Neon™ Transfection System 100 µl Kit
5	Neon™ Transfection System 10 µl Kit
6	Lipofectamine™ CRISPRMAX™ Cas9 Transfection Reagent
7	PES Syringe Filters
8	PES Syringe Filters
9	TrueCut™ Cas9 Protein v2
10	GeneArt™ Genomic Cleavage Detection Kit
11	CD27 Monoclonal Antibody (LG.7F9)
12	Alexa Fluor™ 594 Goat anti-Rat IgG
13	Alexa Fluor™ 647 anti-Mouse IgG
14	PureLink™ HiPure Plasmid Midiprep Kit
15	Opti-MEM™
16	Generic
17	Protein electrophoresis system
18	Wet/tank transfer system
19	Novolink™ Polymer Detection Systems
20	RNeasy Plus Mini Kit (50)
21	BIOLEGEND ITEM
22	APC anti-human CD11c
23	FITC anti-human HLA-DR
24	PE anti-human CD80
25	PE/Cyanine7 anti-human CD86
26	APC/Cyanine7 anti-human CD14
27	Alexa Fluor® 700 anti-human CD40
28	Brilliant Violet 421™ anti-human CD197 (CCR7)
29	Purified anti-human CD45 Antibody
30	7-AAD Viability Staining Solution
31	anti-mouse CD19 Antibody
32	anti-mouse/human CD207
33	Purified anti-mouse CD20 Antibody
34	Brilliant Violet 421 anti-rat IgG
35	HiMedia Laboratories
36	RPMI 1640
37	RPMI 1640
38	Antibiotic Solution 100×
39	FBS
40	EZkill™ Mycoplasma Elimination Kit
41	Carbinol LR
42	Hi-Quanti One Step SYBr Based RT-PCR Kit
43	HiScript One Step RT-PCR Kit (Semi-Q PCR Based)
44	5X Protein Loading Buffer (Reducing)
45	Acrylamide
46	TRASONS ITEM
47	Tissue Culture Petri dish 35 mm
48	Tissue Culture Petri dish 60 mm
49	Microtips 0.2-10 µl
50	Microtips 2-200 µl
51	Microtips 200-1000 µl
52	Microcentrifuge tube 1.5 ml
53	Cryovial 1.8 ml
54	Corning
55	Corning 6-well Plate
56	Corning 12 Well Plate
57	Corning 24 Well Plate
58	Corning cell scrapers
59	Corning Serological Pipets5 mL
60	Corning serological pipettes 10 ml
61	Corning serological pipettes 25 ml
62	Corning® 25cm² Cell Culture Flask with Vent Cap
63	Corning® 75 cm² U-shape cell culture flask, canted neck
64	Cell Signaling Technology
65	Apoptosis Antibody Sampler Kit
66	Calreticulin (D3E6) XP® Rabbit mAb
67	CTLA-4 (E2V1Z) Rabbit mAb
68	Jurkat Apoptosis Cell Extracts
69	Cell Fractionation Kit
70	Merck-Millipore Manufactured, commissioned Reagents and Kits
71	Sodium azide
72	DPX Mountant for histology
73	Paraplast Plus
74	Tipiracil hydrochloride
75	Ficoll®-Paque Premium
76	Xylenes
77	Human Serum
78	Predesigned CRISPR gRNA with positive control