Procurement Of Mammalian Gene Expressing Construct Procurement Of Mammalian Gene Expressing Construct , Mammalian Gene Expressing Construct -Subcloning And Codon Optimization Of Genes For Mammalian And Bacterial Expression, Subcloning Vector Pcd
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Bid Submission
16 Aug 2022
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Bank guarantee accepted
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Non-refundable
Tender Type
Online
Tender Overview
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NCB
Bidding Type
Tender
Location / State
chennai → Tamil Nadu
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Not Available
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Project Description
Procurement Of Mammalian Gene Expressing Construct Procurement Of Mammalian Gene Expressing Construct , Mammalian Gene Expressing Construct -Subcloning And Codon Optimization Of Genes For Mammalian And Bacterial Expression, Subcloning Vector Pcdna 3.1;Pqe80lall The Sequences Should Be Codon-Optimized For Higher-Level Expression ( Soluble Form ) In E. Coli Using Gene Optimizer Technology Software And It Should Be Part Of The Synthetic Construct Optimization. Gene Number 2 To 9 Should Be Cloned In The Pqe80l Using 5’ Bamh1 / 3’Kpni. Gene Number 1 Should Be Cloned In The Pqe80l And Petsumo Or Pet151 Using 5’ Ecor1 / 3’Kpni. , Gene 1 Gaattc Attaaa Gag Gag Aaa Ttaact Atg Aga
BOQ
| Sl. No. | Item Description |
| 1 | Mammalian gene expressing construct -subcloning and codon optimization of genes for mammalian and bacterial expression, subcloning vector pCDNA 3.1;pQE80LAll the sequences should be codon-optimized for higher-level expression (soluble form) in E. coli using gene Optimizer technology software and it should be part of the synthetic construct optimization. Gene number 2 to 9 should be cloned in the pQE80L using 5’ BamH1/3’KpnI. Gene number 1 should be cloned in the pQE80L and pETSUMO or pET151 using 5’ EcoR1/3’KpnI. |
| 2 | Gene 1GAATTC ATTAAA GAG GAG AAA TTAACT ATG AGA GGATCG QLSYGYDEKSTGGISVPGPCCGPPGPPGPPGPPGPPGSPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPAGERGEKGEPGTQGAKGDRGETGPVGPRGERGEAGPAGKDGERGPVGPAGKDGQNGQDGLPGKDGKDGQNGKDGLPGKDGKDGQNGKDGLPGKDGKDGQDGKDGLPGKDGKDGLPGKDGKDGQPGKPGKYGPPGPPGPPGPPGPPGSPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPAGERGEKGEPGTQGAKGDRGETGPVGPRGERGEAGPAGKDGERGPVGPAGKDGQNGQDGLPGKDGKDGQNGKDGLPGKDGKDGQNGKDGLPGKDGKDGQDGKDGLPGKDGKDGLPGKDGKDGQPGKPGKYGPPGPPGPPGPPGPPGPCCSAGFDFSFLPQPPQEKAHDGGRYYRA |
| 3 | Gene 2.DDDDKGPCCGPPGPPGPPGPPGPPGERGPPGPQGARGLPGAPGQMGPRGLPGERGRPGAPGPAGARGEPGAPGSKGDTGAKGEPGPVGVQGPPGPAGEEGKRGARGEPGPTGPAGPKGSPGEAGRPGEAGLPGPPGPPGPPGPPGPPGERGPPGPQGARGLPGAPGQMGPRGLPGERGRPGAPGPAGARGEPGAPGSKGDTGAKGEPGPVGVQGPPGPAGEEGKRGARGEPGPTGPAGPKGSPGEAGRPGEAGLPGPPGPPGPPGPPGPPGPCCGYIPEAPRDGQAYVRKDGEWVLLSTFL |
| 4 | Gene 3.GPCCGPPGPPGPPGPPGPPGSPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPPGPPGPPGPPGPPGSPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPPGPPGPPGPPGPPGPCCGYIPEAPRDGQAYVRKDGEWVLLSTFL |
| 5 | Gene 4. Scl2 three domains with 41 GXY repeats similar to Col108 GPCCGPPGPPGPPGPPGPPGSPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPPGPPGPPGPPGPPGSPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPPGPPGPPGPPGPPGSPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPPGPPGPPGPPGPPGPCCGYIPEAPRDGQAYVRKDGEWVLLSTFL |
| 6 | Gene 5.DDDDKGPCCGPPGPPGPPGPPGPPGSPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPAGERGEKGEPGTQGAKGDRGETGPVGPRGERGEAGPAGKDGERGPVGPAGKDGQNGQDGLPGKDGKDGQNGKDGLPGKDGKDGQNGKDGLPGKDGKDGQDGKDGLPGKDGKDGLPGKDGKDGQPGKPGKYGPPGPPGPPGPPGPPGSPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPAGERGEKGEPGTQGAKGDRGETGPVGPRGERGEAGPAGKDGERGPVGPAGKDGQNGQDGLPGKDGKDGQNGKDGLPGKDGKDGQNGKDGLPGKDGKDGQDGKDGLPGKDGKDGLPGKDGKDGQPGKPGKYGPPGPPGPPGPPGPPGPCCGYIPEAPRDGQAYVRKDGEWVLLSTFL |
| 7 | Gene 6. DDDDKQLSYGYDEKSTGGISVPGPPGPPGPPGPPGPPGSPGLPGPRGEQGPTGPTGPAGPRGLQGYQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGYPGKDGEAGAQGPAGPMGPAGERGEKGEPGTQGAKGDRGETGPVGPRGERGEAGPAGKDGERGPVGPAGKDGQNGQDGYPGKDGKDGQNGKDGYPGKDGKDGQNGKDGYPGKDGKDGQDGKDGYPGKDGKDGYPGKDGKDGQPGKPGKYGPPGPPGPPGPPGPPGSPGLPGPRGEQGPTGPTGPAGPRGLQGYQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGYPGKDGEAGAQGPAGPMGPAGERGEKGEPGTQGAKGDRGETGPVGPRGERGEAGPAGKDGERGPVGPAGKDGQNGQDGYPGKDGKDGQNGKDGYPGKDGKDGQNGKDGYPGKDGKDGQDGKDGYPGKDGKDGYPGKDGKDGQPGKPGKYGPPGPPGPPGPPGPPGPCCGYIPEAPRDGQAYVRKDGEWVLLSTFLSAGFDFSFLPQPPQEKAHDGGRYYRA |
| 8 | Gene 7GAATTC ATTAAA GAG GAG AAA TTAACT ATG AGA GGATCG GPCCGPPGPPGPPGPPGPPGSPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPAGERGEKGEPGTQGAKGDRGETGPVGPRGERGEAGPAGKDGERGPVGPAGKDGQNGQDGLPGKDGKDGQNGKDGLPGKDGKDGQNGKDGLPGKDGKDGQDGKDGLPGKDGKDGLPGKDGKDGQPGKPGKYGPPGPPGPPGPPGPPGSPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPAGERGEKGEPGTQGAKGDRGETGPVGPRGERGEAGPAGKDGERGPVGPAGKDGQNGQDGLPGKDGKDGQNGKDGLPGKDGKDGQNGKDGLPGKDGKDGQDGKDGLPGKDGKDGLPGKDGKDGQPGKPGKYGPPGPPGPPGPPGPPGPCCGYIPEAPRDGQAYVRKDGEWVLLSTFL |
| 9 | Gene 8.GPCCQLSYGYDEKSTGGISVPGPPGPPGPPGPPGPPGSPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPAGERGEKGEPGTQGAKGDRGETGPVGPRGERGEAGPAGKDGERGPVGPAGKDGQNGQDGLPGKDGKDGQNGKDGLPGKDGKDGQNGKDGLPGKDGKDGQDGKDGLPGKDGKDGLPGKDGKDGQPGKPGKYGPPGPPGPPGPPGPPGPCCGYIPEAPRDGQAYVRKDGEWVLLSTFLSAGFDFSFLPQPPQEKAHDGGRYYRA |
| 10 | Gene 9. GPCCQLSYGYDEKSTGGISVPGPPGPPGPPGPPGPPGSPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPAGERGEKGEPGTQGAKGDRGETGPVGPRGERGEAGPAGKDGERGPVGPAGKDGQNGQDGLPGKDGKDGQNGKDGLPGKDGKDGQNGKDGLPGKDGKDGQDGKDGLPGKDGKDGLPGKDGKDGQPGKPGKYGPPGPPGPPGPPGPPGSPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPAGERGEKGEPGTQGAKGDRGETGPVGPRGERGEAGPAGKDGERGPVGPAGKDGQNGQDGLPGKDGKDGQNGKDGLPGKDGKDGQNGKDGLPGKDGKDGQDGKDGLPGKDGKDGLPGKDGKDGQPGKPGKYGPPGPPGPPGPPGPPGPCCGYIPEAPRDGQAYVRKDGEWVLLSTFLSAGFDFSFLPQPPQEKAHDGGRYYRA |
| 11 | Packing & Forwarding, Transportation charges |
AI Tender Summary
Tender Timeline
Aug 01, 2022
11:30 IST
Tender Published
Tender notice published.
CompletedAug 16, 2022
17:00 IST
Bid Submission Deadline
Online submission via eProcurement portal.
CompletedAug 17, 2022
Bid Opening Date
Technical bids will be opened and evaluated.
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