}
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    • Procurement Of Mammalian Gene Expressing Construct Procurement Of Mammalian Gene Expressing Construct , Mammalian Gene Expressing Construct -Subcloning And Codon Optimization Of Genes For Mammalian And Bacterial Expression, Subcloning Vector Pcd, chennai-Tamil Nadu

    Council Of Scientific And Industrial Research-CSIR has published Procurement Of Mammalian Gene Expressing Construct Procurement Of Mammalian Gene Expressing Construct , Mammalian Gene Expressing Construct -Subcloning And Codon Optimization Of Genes For Mammalian And Bacterial Expression, Subcloning Vector Pcd. Submission Date for this Tender is 16-08-2022. Laboratory Equipment Tenders in chennai Tamil Nadu. Bidders can get complete Tender details and download the document.




    Tender Notice

    33350817
    Procurement Of Mammalian Gene Expressing Construct Procurement Of Mammalian Gene Expressing Construct , Mammalian Gene Expressing Construct -Subcloning And Codon Optimization Of Genes For Mammalian And Bacterial Expression, Subcloning Vector Pcd
    Open Tender
    Indian
    Tamil Nadu
    chennai
    16-08-2022

    Tender Details

    Procurement Of Mammalian Gene Expressing Construct Procurement Of Mammalian Gene Expressing Construct , Mammalian Gene Expressing Construct -Subcloning And Codon Optimization Of Genes For Mammalian And Bacterial Expression, Subcloning Vector Pcdna 3.1;Pqe80lall The Sequences Should Be Codon-Optimized For Higher-Level Expression ( Soluble Form ) In E. Coli Using Gene Optimizer Technology Software And It Should Be Part Of The Synthetic Construct Optimization. Gene Number 2 To 9 Should Be Cloned In The Pqe80l Using 5’ Bamh1 / 3’Kpni. Gene Number 1 Should Be Cloned In The Pqe80l And Petsumo Or Pet151 Using 5’ Ecor1 / 3’Kpni. , Gene 1 Gaattc Attaaa Gag Gag Aaa Ttaact Atg Aga

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    BOQ Items

    Name of Work:Mammalian gene expressing construct -subcloning and codon optimization of genes for mammalian and bacterial expression, subcloning vector pCDNA 3.1;pQE80L
    Sl. No. Item Description
    1Mammalian gene expressing construct -subcloning and codon optimization of genes for mammalian and bacterial expression, subcloning vector pCDNA 3.1;pQE80LAll the sequences should be codon-optimized for higher-level expression (soluble form) in E. coli using gene Optimizer technology software and it should be part of the synthetic construct optimization. Gene number 2 to 9 should be cloned in the pQE80L using 5’ BamH1/3’KpnI. Gene number 1 should be cloned in the pQE80L and pETSUMO or pET151 using 5’ EcoR1/3’KpnI.
    2Gene 1 GAATTC ATTAAA GAG GAG AAA TTAACT ATG AGA GGATCG QLSYGYDEKSTGGISVPGPCCGPPGPPGPPGPPGPPGSPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPAGERGEKGEPGTQGAKGDRGETGPVGPRGERGEAGPAGKDGERGPVGPAGKDGQNGQDGLPGKDGKDGQNGKDGLPGKDGKDGQNGKDGLPGKDGKDGQDGKDGLPGKDGKDGLPGKDGKDGQPGKPGKYGPPGPPGPPGPPGPPGSPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPAGERGEKGEPGTQGAKGDRGETGPVGPRGERGEAGPAGKDGERGPVGPAGKDGQNGQDGLPGKDGKDGQNGKDGLPGKDGKDGQNGKDGLPGKDGKDGQDGKDGLPGKDGKDGLPGKDGKDGQPGKPGKYGPPGPPGPPGPPGPPGPCCSAGFDFSFLPQPPQEKAHDGGRYYRA
    3Gene 2. DDDDKGPCCGPPGPPGPPGPPGPPGERGPPGPQGARGLPGAPGQMGPRGLPGERGRPGAPGPAGARGEPGAPGSKGDTGAKGEPGPVGVQGPPGPAGEEGKRGARGEPGPTGPAGPKGSPGEAGRPGEAGLPGPPGPPGPPGPPGPPGERGPPGPQGARGLPGAPGQMGPRGLPGERGRPGAPGPAGARGEPGAPGSKGDTGAKGEPGPVGVQGPPGPAGEEGKRGARGEPGPTGPAGPKGSPGEAGRPGEAGLPGPPGPPGPPGPPGPPGPCCGYIPEAPRDGQAYVRKDGEWVLLSTFL
    4Gene 3. GPCCGPPGPPGPPGPPGPPGSPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPPGPPGPPGPPGPPGSPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPPGPPGPPGPPGPPGPCCGYIPEAPRDGQAYVRKDGEWVLLSTFL
    5Gene 4. Scl2 three domains with 41 GXY repeats similar to Col108 GPCCGPPGPPGPPGPPGPPGSPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPPGPPGPPGPPGPPGSPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPPGPPGPPGPPGPPGSPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPPGPPGPPGPPGPPGPCCGYIPEAPRDGQAYVRKDGEWVLLSTFL
    6Gene 5. DDDDKGPCCGPPGPPGPPGPPGPPGSPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPAGERGEKGEPGTQGAKGDRGETGPVGPRGERGEAGPAGKDGERGPVGPAGKDGQNGQDGLPGKDGKDGQNGKDGLPGKDGKDGQNGKDGLPGKDGKDGQDGKDGLPGKDGKDGLPGKDGKDGQPGKPGKYGPPGPPGPPGPPGPPGSPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPAGERGEKGEPGTQGAKGDRGETGPVGPRGERGEAGPAGKDGERGPVGPAGKDGQNGQDGLPGKDGKDGQNGKDGLPGKDGKDGQNGKDGLPGKDGKDGQDGKDGLPGKDGKDGLPGKDGKDGQPGKPGKYGPPGPPGPPGPPGPPGPCCGYIPEAPRDGQAYVRKDGEWVLLSTFL
    7Gene 6. DDDDKQLSYGYDEKSTGGISVPGPPGPPGPPGPPGPPGSPGLPGPRGEQGPTGPTGPAGPRGLQGYQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGYPGKDGEAGAQGPAGPMGPAGERGEKGEPGTQGAKGDRGETGPVGPRGERGEAGPAGKDGERGPVGPAGKDGQNGQDGYPGKDGKDGQNGKDGYPGKDGKDGQNGKDGYPGKDGKDGQDGKDGYPGKDGKDGYPGKDGKDGQPGKPGKYGPPGPPGPPGPPGPPGSPGLPGPRGEQGPTGPTGPAGPRGLQGYQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGYPGKDGEAGAQGPAGPMGPAGERGEKGEPGTQGAKGDRGETGPVGPRGERGEAGPAGKDGERGPVGPAGKDGQNGQDGYPGKDGKDGQNGKDGYPGKDGKDGQNGKDGYPGKDGKDGQDGKDGYPGKDGKDGYPGKDGKDGQPGKPGKYGPPGPPGPPGPPGPPGPCCGYIPEAPRDGQAYVRKDGEWVLLSTFLSAGFDFSFLPQPPQEKAHDGGRYYRA
    8Gene 7 GAATTC ATTAAA GAG GAG AAA TTAACT ATG AGA GGATCG GPCCGPPGPPGPPGPPGPPGSPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPAGERGEKGEPGTQGAKGDRGETGPVGPRGERGEAGPAGKDGERGPVGPAGKDGQNGQDGLPGKDGKDGQNGKDGLPGKDGKDGQNGKDGLPGKDGKDGQDGKDGLPGKDGKDGLPGKDGKDGQPGKPGKYGPPGPPGPPGPPGPPGSPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPAGERGEKGEPGTQGAKGDRGETGPVGPRGERGEAGPAGKDGERGPVGPAGKDGQNGQDGLPGKDGKDGQNGKDGLPGKDGKDGQNGKDGLPGKDGKDGQDGKDGLPGKDGKDGLPGKDGKDGQPGKPGKYGPPGPPGPPGPPGPPGPCCGYIPEAPRDGQAYVRKDGEWVLLSTFL
    9Gene 8. GPCCQLSYGYDEKSTGGISVPGPPGPPGPPGPPGPPGSPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPAGERGEKGEPGTQGAKGDRGETGPVGPRGERGEAGPAGKDGERGPVGPAGKDGQNGQDGLPGKDGKDGQNGKDGLPGKDGKDGQNGKDGLPGKDGKDGQDGKDGLPGKDGKDGLPGKDGKDGQPGKPGKYGPPGPPGPPGPPGPPGPCCGYIPEAPRDGQAYVRKDGEWVLLSTFLSAGFDFSFLPQPPQEKAHDGGRYYRA
    10Gene 9. GPCCQLSYGYDEKSTGGISVPGPPGPPGPPGPPGPPGSPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPAGERGEKGEPGTQGAKGDRGETGPVGPRGERGEAGPAGKDGERGPVGPAGKDGQNGQDGLPGKDGKDGQNGKDGLPGKDGKDGQNGKDGLPGKDGKDGQDGKDGLPGKDGKDGLPGKDGKDGQPGKPGKYGPPGPPGPPGPPGPPGSPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPAGERGEKGEPGTQGAKGDRGETGPVGPRGERGEAGPAGKDGERGPVGPAGKDGQNGQDGLPGKDGKDGQNGKDGLPGKDGKDGQNGKDGLPGKDGKDGQDGKDGLPGKDGKDGLPGKDGKDGQPGKPGKYGPPGPPGPPGPPGPPGPCCGYIPEAPRDGQAYVRKDGEWVLLSTFLSAGFDFSFLPQPPQEKAHDGGRYYRA
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