Work Of Processing And Conversion Of 325000 Kg Old Damaged/Burnt Copper Coils Into Etp Grade Dpc Copper Winding Wire And Dpc Copper Strips., Meerut-Uttar Pradesh
TDR : 33551682
Tender Notice
BOQ
Expired
Meerut
Uttar Pradesh
Work Of Processing And Conversion Of 325000 Kg Old Damaged/Burnt Copper Coils Into Etp Grade Dpc Copper Winding Wire And Dpc Copper Strips.
Name of Work:Mammalian gene expressing construct -subcloning and codon optimization of genes for mammalian and bacterial expression, subcloning vector pCDNA 3.1;pQE80L
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Item Description
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Mammalian gene expressing construct -subcloning and codon optimization of genes for mammalian and bacterial expression, subcloning vector pCDNA 3.1;pQE80LAll the sequences should be codon-optimized for higher-level expression (soluble form) in E. coli using gene Optimizer technology software and it should be part of the synthetic construct optimization. Gene number 2 to 9 should be cloned in the pQE80L using 5’ BamH1/3’KpnI. Gene number 1 should be cloned in the pQE80L and pETSUMO or pET151 using 5’ EcoR1/3’KpnI.
Gene 4. Scl2 three domains with 41 GXY repeats similar to Col108 GPCCGPPGPPGPPGPPGPPGSPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPPGPPGPPGPPGPPGSPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPPGPPGPPGPPGPPGSPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPPGPPGPPGPPGPPGPCCGYIPEAPRDGQAYVRKDGEWVLLSTFL
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