Supply Of Synthesis Of Dna With Desired Sequences And Subcloning Into Expression Supply Of Synthesis Of Dna With Desired Sequences And Subcloning Into Expression Vectors , Supply Of Synthesis Of Dna With Desired Sequences And Subcloning Into Expression

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Supply Of Synthesis Of Dna With Desired Sequences And Subcloning Into Expression Supply Of Synthesis Of Dna With Desired Sequences And Subcloning Into Expression Vectors , Supply Of Synthesis Of Dna With Desired Sequences And Subcloning Into Expression Vectors. Technical Specifications Are - Dna Sequences Must Be Codon Optimised For Protein Expression In Escherichia Coli. Less Than 2 Kilobases Of Each Desired Gene Has To Be Synthesized And Subcloned. Subcloning In Fifteen Standard Pet Or Equivalent Vectors Should Be Done, Having Dna Restriction Sites In Non-Coding Portion And Polyhistidine Or Mbp Or Gst Tags At N- Or C-Terminal With Protease Cleavage Sites. More Than 4 Micrograms Of Each Hplc Purified Plasmid Containing Gene With Vector, Should Be Provided In Freeze Dried Form. Plasmid Vectors And Sequencing Primers Should Be Arranged By The Supplier. The Vendor Should Have Past Experience In Gene Synthesis And Cloning And Provide Proof. Dna Sequencing ( Both Forward And Reverse ) Should Be Done, Ensuring Full Accuracy. Provision Should Be There To Replace Defective Products Free Of Cost. Sequencing Results And Chromatograms With Data Should Be Provided To Us By The Vendor.