Supply Of Plasticwares /Cell Culture Reagents /Chemicals /Antibodies -

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Supply Of Plasticwares /Cell Culture Reagents /Chemicals /Antibodies -1 Trypsin-EDTA Solution 10X W/2.5% Trypsin (TCL070) 2 Antibiotic Solution 100X Liquid w/ 10,000 U Penicillin and 10mg Streptomycin per ml in 0.9% normal saline (A001-50ML) 3 Fetal Bovine Sera, Gamma Irradiated, Sterile Filtered 4 Nutrient Mixture FModification W/ L-Glut (Cat.No: AL106S) 5 RPMI-1640 With 2mM L-Glutamine, 4.5gms Glucose per liter, 10mM HEPES, 1.5gms per liter Sodium bicarbonate and 1mM Sodium pyruvate 1X Liquid Cell Culture medium (Cat.No: AL162S) cell culture plastic wares -1 MICRO TIPS PP autoclavable 200- 1000UL 2 MICRO TIPS PP Autoclavable 2-200UL 3 MICRO TIPS PP Autoclavable 0.2 - 10UL 4 SAFESKIN Nitrile Gloves 9.5 Length SMALL 5 Syringe driven filter- 0.22μ 6 Cellulose Nitrate 0.22μ Membrane Diameter: 47 7 T25 culture flask, vented cap CC treated, autoclavable 8 0.2ml Tube with cap PP Autoclavable 0.2ml 9 Eppendorf 1.5ml tubes, Autoclavable 10 Eppendorf 2.0ml tubes, Autoclavable 11 Cell culture dish 60x15mm, Working Volume, CC Treated, Autoclavable Cell culture Dyes- 1 MTT 2 Coomassiee brilliant blue G250 3 DAPI TC229 4 Hoechst 14530 Sigma-Aldrich bisBenzimide H 33258 5 Propidium iodide Cell Culture Tested Product Code: TC252 6 Acridine orange Product Code: TC262 7 MB071 Ethidium bromide, For Molecular Biology 8 JC-1 stain 420200 Sigma-Aldrich JC-1 - CAS 47729-63-5 - Calbiochem Chemicals- 1 Sodium chloride 2 Potassium chloride 3 Methanol 4 Paraformaldehyde 5 Isopropanol 6 Ethanol 7 Sodium hypochlorite Gene expression - 1 TRIzol 2 cDNA synthesis kit 3 RNA isolation from mammalian cells Mini kit 4 RT-PCR kit 5 Top Taq master mix 6 100bp DNA Ladder M MBT130-50LN 50 Lanes Protein expression- 1 Sodium Dodecyl Sulfate (SDS) 2 Acrylamide 3 Bisacrylamide 4 Tris base 5 Glycerol 6 Bovine serum albumin 7 Protease inhibitor cocktail 8 Ammonium persulfate 9 TEMED 10 PVDF membrane Western Blotting Membranes roll W × L 30 cm × 3.00 m, 11 Nitrocellulose Blotting Membrane Roll Dimensions: 30cm x 3m, Pore size: - 1 roll 12 Protein dual stained ladder Antibodies-1 GAPDH Rabbit mAb 2 Cleaved caspase 3 Rabbit mAb 3 Caspase 9 Rabbit mAb 4 Caspase 8 Rabbit mAb 5 BAK 6 mTOR Antibody 7 Bcl-2 (124) 8 Phospho-mTOR 9 Cyclin D1 Antibody 10 Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) 11 Anti-mouse IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) 12 Anti-rabbit IgG HRP 13 Anti-Mouse IgG HRP