Item Details: Kit for Skeletal Muscle Dissociation and others
Sl. No.
Item Description
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Skeletal Muscle Dissociation Kit suitable for mouse and rat tissue for 50 digestions of 2.5 ml: Containing: 2 vials of Enzyme D (lyophilized powder), 2.5 mL of Enzyme P, 2 vials of Enzyme A (lyophilized powder),2× 1 mL of Buffer A.Enzyme P suitable for storage at –20 °C and other reagents suitable for storage at2–8 °C.
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MTT Cell Viability Assay KitContaining 10 vials of 5 mg MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and 10 vials of 1 mg SDS sodium dodecyl sulfate. Detection Method: Colorimetric, Suitable for: 96 well Microplate Reader, sufficient for 1000 tests.
ATP quantification Kit: Containing:a) D-Luciferin: 5 vials, each containing 3 mg of lyophilized powder,b)Luciferase, firefly recombinant :40 µL of a 5 mg/mL solution in 25 mM Tris-acetate, pH 7.8, 0.2 M ammonium sulfate, 15% (v/v) glycerol and 30% (v/v) ethylene glycol, c) Dithiothreitol:25 mg, d) Adenosine:5'-triphosphate (ATP)400 µL of a 5 mM solution in TE buffer, e) 20X Reaction Buffer: 10 mL of 500 mM Tricine buffer, pH 7.8, 100 mM MgSO4, 2 mM EDTA and 2 mM sodium azide.Sufficient for 200-1000 reactions.
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GlutathioneColorimetric Detection KitOxidized Glutathione Standard;250 µMoxidized glutathione in a specialstabilizing solution350 µL Clear 96-well Half Area P late4 plates Detection ReagentConcentrate; in DMSO1 mLAssayBuffer;phosphate bufferwith chelatorsand stabilizers225 mLNADPH Concentrate:reduced ß-nicotinamide adenine dinucleotide2’-phosphate1 mLGlutathioneReductaseConcentrate1 mLSufficient fro 4 x 96 reactions
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