Providing Gc-Ms/Ms With Fid

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Providing Gc-Ms/Ms With Fid 1.1 Equipment Configuration A. A Latest and highly sensitive Gas Chromatograph with tripe/ Tandem quadruple mass spectrometer system required with software, auxiliary equipment, accessories and data base. 1.2 Area of analysis The equipment shall have capability to quantify at 1/4th of the maximum residue limit set by national and international regulatory authority for milk and milk products. a. Analysis of pesticides in milk and milk products as per FSSAI. b. Milk fat purity by Triglycerides as per ISO 17678/2010 on GC-FID c. Fatty acids in milk and milk products 2 Gas Chromatograph (GC) 2.1 Compatibility/capabilitie s a. A benefit top gas chromatograph system shall be compatible with offered mass spectrometer, Flame Ionization detector (FID), Auto sampler, Inlets ( 02 nos), columns (min 3 Nos.) and all other necessary consumables for operation of one year. b. Oven of Gas chromatograph shall have enough space to install two columns and other accessories. 2.2 Gas Supplies a. The gases and flow rate requirements on 24 hrs. of continuous working shall be specified with the offer. b. The purification filters required for all the gases to be used in equipment shall be included c. System shall have Electronic pneumatic/ pressure controls of all the gasses. 2.3 Auto sampler a. Auto sampler automatic injection devices with minimum 15 or more vial tray. b. It shall have reproducibility < 0.5% RSD when injected Iul. c. Auto sampler shall have provision for minimizing the carry over. It shall have facility to wash syringe with more than two solvents before and after the injections. 2.4 Inlet (2 No’s) a. System should be offered with two inlets 1) MMI/PTV for pesticide analysis 2) On Column injector for Triglyceride analysis or any other suitable inlet for triglycerides analysis as per ISO 17678:2010 subject to successful demonstration. b. System shall have advanced electronic flow 2.5 Column Oven a. System shall have provision to install two columns simultaneously during routine work b. Operating temp range of oven from near ambient to 4500C c. Support 15 oven ramps or better in a single run d. Ramp rate shall go up to 1000C/min or more e. Supplier shall supply 1) Two suitable columns for pesticides ( 30 Meter Length) for use on GCMS/MS. 2) One suitable column for Fatty Acid-100 M Length. 3) One suitable column for Triglyceride analysis- 5 Meter Length, 0.53 mm ID; 0.17Um with non-polar stationary phase ( as per ISO 17678:2010) 2.6 a. GC should have facility of retention time locking/or other suitable equivalent mechanism to avoid change in retention times. b. The system should have independently heated GC-MS/MS interface to avoid loss in analytes due temperature gradient in after elution from column. 3 Mass Spectrometer 3.1 Ion Source a. The equipment quoted with EI mode of ionization. b. The system shall have ability to program ion source temperature up to 350 •c or better 3.2 Mass filters/analyser a. Triple quadruple with suitable mechanism for calibration , auto tune, compound optimisation, quantitation and confirmation b. Quadruple shall be made up of inert material to have better mass transfer efficiency and shall have provision for keeping quadruple clean from dirty matrix c. Collision Energy must be selectable up to 60eV d. System shall analyse masses from 10 to 1050 AMU e. Scan speed up-to 20000 dalton/ sec (u /s) or better . f. Mass stability should be up to 0.10 amu / 24 hrs or better g. MRM speed (transitions/ sec) 800 or more enabling automatically quantifying and confirming more targets in a single method run. h. Minimum MRM Dwell Time must be at least 0.5msec 3.3 Detector a. System should have Electron multiplier detector or photo multiplier detector with Jong life and better sensitivity . b. It shall have digital dynamic range <! 1 X 106 or better 3.4 Calibration of mass spectrometer a. Mass spectrometer shall have suitable mechanism for injecting the calibration solution for calibration system . b. Manufacturer shall provide calibration of the instrument quarterly during warranty period. 3.5 Acquisition mode System shall be capable of acquiring data in full scan, product ion scan, selected ion monitoring and multiple reaction monitoring. 3.6 Sensitivity EI MRM Sensitivity: 1ul of 100 fg/ ul octafluoronaphthalene (OFN) RMS S/ N minimum 30,000: 1 for MS/ MS transition of m / z 272-+222 or Numerically equivalent , if estimated by injecting different amount of concentration of OFN. 3.7 Vacuum System a. The pumps in required numbers to be offered to meet the instrument vacuum requirement and sensitivity. b. All accessories required for the proper functioning of vacuum system shall be supplied. c. Vacuum system shall have proper venting/exhaust system to remove effluents from pumps and sample split out of laboratory . 4 Flame Ionisation Detector FID a. Compatible with capillary columns of different diameters b. Shall have Flame out detection and automatic reignition facility c. Minimum Detectable Level (MDL):< l.5 pg C/s for tridecane d. Linear dynamic range: > 107 e. Temperature: 440 °C or More f. Standard electronic pneumatic control for three gases i.e. Air; Hydrogen and Makeup gas (N2 or He) shall be available for operation . 5 Computer Work station with System Software 5.1 Computer work station Latest model of branded computer with preloaded software, laser jet duplex printer shall be offered. The monitor shall have large screen preferably of 24 inches or better. 5.2 Software a. Preloaded fully automated data acquiring & pro cessing software for with original Software CD and license number of Software shall be supplied with the system. b. Software shall have control over all operations of Auto sampler, gas chromatography, mass spectrometer and printer simultaneously or separately. 5.3 Software features a. Software shall have necessary functions to carry out data acquisition peak integration, quantification for accurate quantitation and confirmations of the analytes of interest/ indicating ion rations. b. Software shall perform simultaneous SIM and SCAN mode of data acquisition. c. Software shall have auto flagging facilities in certain parameters as 1. Auto flagging for the ion ratio which falls out of the confirmation criteria 2. Auto flagging for the concentration which is falling below limit of quantitation 3. Auto flagging for recovery / QC values which is falling out of the defined range of acceptance 5.4 Databases / Libraries a. Bidder shall provide a database of GC amenable compounds like Pesticides, PCBs, PAH, Dioxins, other contaminants etc. Database should have information like molecular formula, mono isotopic mass, parent and daughter ions cone voltage, collision energy, and other parameters required to standardise analysis method for any compound b. NIST Library. 6 Calibration solution/standards , consumables and accessories 6.1 Toolkits/ consumable kits/calibration kit/spares The supplier shall include and specify the kits of relevant equipment parts and accessories along with tool kit. 6.2 Calibration solution/ standards All calibration s tandar ds for mass spectrometer I gas chromatograph / auto sampler shall be offered . 7 Installation 7.1 Pre-installation The party need to submit the preinstallation requirement with the offer 7.2 Installation The installation to be undertaken while ensuring all the criteria for compliance as per specification are met and demonstrated . 7.3 IQ/ OQ/ PQ Supplier shall perform all these tests during the installation . 8 Warranty 8.1 Warranty Supplier shall provide comprehensive warranty for one year covering all parts of equipment. The warranty of equipment would be extended by a month, if equipment has been down for more than 10 days in a year, whereas, warranty of equipment would be extended by 1 year, if equipment has been down for more than 30 days due to malfunctioning of any part supplied by bidder during warranty period. 8.2 AMC 1 year free comprehensive maintenance be provided and AMC charges from 3rd year onwards should be quoted separately. 9 Training Operation and application training a. One week operational training after installation covering GCMS/ MS; GCFID, software features, instrument troubleshooting and maintenance . b. Supplier shall provide one week application training on method development and standardization 10 Method Set Up Bidder shall develop the methods as per application requirement of tender after installation. It shall Cover equipment optimization, and sample preparation. It shall meet the performance criteria. a) Pesticides in two products i.e. Liquid milk and ghee or any product suggested by the end user as per FSSAI requirement for GC amenable pesticides (Refer Sr. no. 14). b) Standardisation of method for analysis of triglycerides profile as per IS017678/2010 in milk, milk products. c) Standardisation of method for analysis of fatty acid profile as per AOAC 996.06 11 Service & Supply 11.1 Local service facility a. The supplier shall have service facility and trained engineers located in nearby metros to provide breakdown services within 24-48hrs of complaint registration. b. The supplier shall have application laboratory in India to support the application requirement and training needs. c. Supplier shall be responsible for providing the service during the warranty and AMC period for the instruments and accessories offered from other manufacturers also. 11.2 Stock inventory The supplier shall keep inventory of spares and consumables in India for the repair of equipment. 11.3 Number of installations The supplier shall provide a list of installations of GC- triple quadruple mass spectrometer in food testing laboratories from government and private sector during last three years with contact details. 11.4 Warranty extension The warranty of equipment would be extended by a month, if equipment has been down for more than 10 days in a year, it would be extended by 1 year, if equipment has been down for more than 30 days due to malfunctioning of the any part supplied by bidder or want of spares. 12 Performance verification and compliance 12.1 Demonstration and Evaluation a) We reserves the right to verify the performance of equipment at any stage of purchase process a. The bidder are requested to arrange all the pre-requisite and set up applications (as per Sr. no. 1.2a) at their facility for timely, successful demonstration and evaluation. 12 .2 Compliance a. The bidders are requested to provide point wise compliance for each point in specification along with documentary evidences. b. The reference of compliance information in submitted offer shall be clearly specified . c. The offer with incomplete/ untraceable information may not be considered. 12.3 Canvassing a. Any type of unwarranted canvassing for selection of instrument may lead to rejection of the offer. 12.4 No offering of refurbished / assembled / demo modules a. No part of the offered instrument shall be refurbished or used in demonstration purpose. Kindly submit undertaking. 13.0 General conditions It is the responsibility of vendor to supply a complete solution / unit for the analysis. Vendor should be able to supply all the spares I consumable required for repair at least 8- 10 years after installation. 14.0 Reference to Sr. No. 10.0 the list of Pesticides and LOQ to be set up. Requirement of GC amenable pesticides as per FSSAI in milk and milk products Sr. No Name Of compound MRL (mg/ kg) PPM Limit of Quantification 1 Aldrin and dieldrin 0.01 1/4th of Maximum 2 Chlordane 0.01 Residue Limit 3 DDT, DDD & DDE 0.01 4 Fenithrothion 0.01 5 Heptachlor 0.01 6 Lindane 0.01 7 Chlorpyriphos 0.02 8 Cypermethrin 0.05 9 Fenthion 0.01 10 Fenvalerate 0.01 11 Phorate 0.05 12 Pirimiphos-methyl 0.05 13 Endosulfan (sum of alpha, beta isomers and endosulfan sulfate expresses as endosulfan) 0.01 14 Heptachlor Epoxide 0.01 15 Diazinon 0.01 16 Parathion methyl (sum of parathion-methyl and paraoxon-methyl expressed as parathion methyl) 0.01 17 Bifenthrin 0.2 18 Chlorothalonil 0.07 19 Deltamethrin 0.05 20 Dichlorvos 0.01 21 Etofenprox 0.02 22 Fenproprathrin 0.1 23 Triacontanol 0.01 24 Metiram as CS2 0.05 25 Mancozeb 0.05 26 Phosphamidon 0.01 27 Captafol 0.01 28 Formothion 0.01 29 Simazine 0.01 Requirement of GC amenable pesticides as per EIC-RMP_2019-20 in milk products Sr. No Name Of compound MRL (μg/ kg) PPB Limit of Quantification 1 Aldrin and dieldrin 6 1/3rd of Maximum 2 Chlordane 2 Residue limit 3 Endosulfan (Sum of Alpha,beta and Sulfate) 50 4 Lindane 1 5 Heptachlor (Sum of heptachlor and epoxide) 4 6 Methoxychlor 10 7 Diazinon 10 8 Fenthion 10 9 Malathion 20 10 Parathion-meth yl 20 11 Phosalone 10 Inductively Coupled Plasma MassSpectrometer (ICP-MS) Sodium Potassium Analyser SPECIFICATION OF GERBER CENTRIFUGE ANALYTICAL WEIGHING BALANCES 220 G