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| 1 | Molecular Biology Regants/Kits/Plasmids (As per RFP) |
| 2 | RNase Inhibitor: Recombinant, murine RNase Inhibitor to provide complete protection against omnipresent RNases (RNases A, B & C). (3000 Units/Pack) |
| 3 | In-Vitro Transcription Kit (100 Rxn/Kit) |
| 4 | cDNA synthesis Kit (100 Rxn/Kit) |
| 5 | Bst 3.0 DNA Polymerase (1600U/Pack) |
| 6 | Lipofectamine 3000 Transfection Reagent (0.1ml/Pack) |
| 7 | RNase Decontamination Solution (RNaseZap) (250ml/Pack) |
| 8 | T4 DNA ligase (20,000 units/Pack or small pack) |
| 9 | Ribonucleotide Solution Mix: Buffered equimolar solution of ribonucleotide triphosphates: rATP, rCTP, rGTP and rUTP, NTP Mix (25 mM of each) (Concentr |
| 10 | Plasmid- pLentiRNAGuide_001-hUG-RfxCas13d-Dr1-BsmB1-EFS-Puro-WPRE, For RfxCas13d compatible cloning of gRNAs (Plasmid in bacteria as agar stab/Pack) |
| 11 | Plasmid- pLentiRNACRISPR_007-TetO-NLS-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast. Expression of RfxCas13d. (Plasmid in bacteria as agar stab/Pack) |
| 12 | Plasmid- pLentiRNACRISPR_006 - hU6-DR_BsmBI-EFS-RfxCas13d-NES-2A-Puro-WPRE. Expresses RfxCas13d. (Plasmid in bacteria as agar stab/Pack) |
| 13 | Plasmid- pLentiRNACRISPR_005 - hU6-DR_BsmBI-EFS-RfxCas13d-NLS-2A-Puro-WPRE. Expresses RfxCas13d & gRNA cloning. Plasmid in bacteria as agar stab/Pack |
| 14 | Plasmid- pET-28b-RfxCas13d-His. Bacterial expression & purification of RfxCas13d protein (Plasmid in bacteria as agar stab/Pack) |
| 15 | Plasmid- pC013-Twinstrep-SUMO-huLwCas13a. For recombinant protein bacterial expression. (Plasmid in bacteria as agar stab/Pack) |
| 16 | Plasmid- pXR003- CasRx gRNA cloning backbone. hU6-driven expression of guide RNAs compatible with CasRx. (Plasmid in bacteria as agar stab/Pack) |
| 17 | Plasmid- pXR004- CasRx pre-gRNA cloning backbone. hU6-driven expression of CasRx compatible gRNA. (Plasmid in bacteria as agar stab/Pack) |
| 18 | Plasmid- pM115- CasRx control presgRNA. U6-driven expression of control presgRNA compatible with CasRx. (Plasmid in bacteria as agar stab/Pack) |
| 19 | Plasmid- pLBH545_Tet-Cas14a1_Locus (Plasmid in bacteria as agar stab/Pack) |
| 20 | Plasmid- pLBH532_MBP-Cas14b1 expression (Plasmid in bacteria as agar stab/Pack) |
| 21 | Dipstick LFA- Lateral flow strips designed to detect biotin and FAM labelled Oligos (100strips/Pack) |
| 22 | Cas13a Protein- Cas13a from Leptotrichia wadeii (strain F0279). 100 microgram/Pack |
| 23 | Cas13d Protein: CasRx, Cas13d from Ruminococcus flavefaciens XPD3002. 100microgram/Pack |
| 24 | Golden Gate Assembly Kit (BsmBI-v2) (20Rxn/Kit) |
| 25 | Gibson Assembly Cloning Kit (10 Rxn/Kit) |
| 26 | Ampicillin powder (1.0Gmx5/Pack) |
| 27 | Kanamycin Powder (1.0Gmx5/Pack) |
| 28 | IPTG (1.0gm/Pack) |
| 29 | Restriction enzymes- BbsI (300Units/Pack) |
| 30 | Restriction enzymes- NcoI (300Units/Pack) |
| 31 | Restriction enzymes- BsmBI (300Units/Pack) |
| 32 | PCR Purification Kit (50Prep/Kit) |
| 33 | Ni-NTA Superflow Columns (12 x 1.5 ml/Pack) |
| 34 | Ni-NTA Superflow (25 ml/Pack) |
