Supply, Installation And Commissioning Of Fast Protein Liquid Chromatography To Recover And Purify Collagen Protein From Tannery Fleshing Waste Supply, Installation And Commissioning Of Fast Protein Liquid Chromatography To Recover And Purify Collagen P, CLRI Chennai-Tamil Nadu

Supply, Installation And Commissioning Of Fast Protein Liquid Chromatography To Recover And Purify Collagen Protein From Tannery Fleshing Waste Supply, Installation And Commissioning Of Fast Protein Liquid Chromatography To Recover And Purify Collagen Protein From Tannery Fleshing Waste , Supply, Installation And Commissioning Of Fast Protein Liquid Chromatography ( Fplc ) To Recover And Purify Collagen Protein From Tannery Fleshing Waste , Name Of The Instrument - Fast Protein Liquid Chromatography System, System Configuration- Automated & Stand-Alone Bench-Top System For Protein Purification Work, Connection Between Pc And Instrument- Usb, System Capabilities - Suitable For Affinity, Ion Exchange And Gel Filtration Chromatography Column, Flow Rate- 0.5 To 5 Ml / Min Or Wider Range With Automatic Gradient Formation Capability ( 5 To 95% Of Buffer B ) , Pressure Of The System- System Should Bear Pressure Range Should Between 0 To 0.5Mpa Along With Pressure Sensor, Viscosity - Sample Bearing Viscosity 0.6-5Cp, Power Supply- 100-240 Vac, 50 To 60 Hz, Power Consumption - 90-100 Va, Inlet Valve - Ptfe Tubing, 1.5-1.8 Mm, Buffer Valve- Peek Tubing, 0.70-0.80 Mm, Outlet And Waste- Etfe Tubing, 1.0-1.10 Mm, Fingertight Connector, 1 / 16”, Sample Tubing - Etfe Tubing, 0.70-0.80 Mm, Fingertight Connector, 1 / 16”, Pump Tubing- Marprene Tubing, 0.80 Mm, Equipment Noise Level - < 60 Db A, Buffer - Alkaline And Acid Mobile Phase, Hplc Grade Water, Sample Volume And Injection Valve - 10Ml, Rotary-Type Manual Valve, Function Sample Injection Through Loop, Sample Injection - Manual, Number Of Injection Ports - 6-8, Wavelength ( Nm ) - Any Fixed Wavelength Between 260-300Nm, Uv Flow Cell - 2 Mm Optical Path Length, 2-5?L Cell Volume, Operating Temperature:4ºc To 32ºc Or Wider, Software - Computer Controlled System Along With Specific Software For Fplc Operation And System Control, Method Editor, Evaluation, Administration And Design Runs. Along With Software, License And Warranty Are Required. Real-Time Process Monitoring And Control Through Touch Screen Display. Computer ( Desktop, I5 ) For Monitoring And Data Processing - Compatible With Software And User Friendly With Fplc System Control, Possibly Upgradable • Processor – Intel I5 10G • Os – Window / Linux / Dos • Monitor – 1 No, Size- 24 Inch • Mouse-1 No, • Keyboard- 1 No, • Cpu- 1 No, 8 Gb Ram, 1 Tb Hard Disk Storage, Pump Type - Peristaltic Pump, Single Channel, Four Roller Pump Head With Low Pulsation, Mixer - Static Mixer ( 0.4 Ml ) Used For Blending Buffers During Gradient Runs. Column ( One Number Each ) - High Load Columns With Preparative Gel Filtration Media Also With A Unique Composite Matrix Of Dextran And Agarose, And A Desalting Column, The Column Matrix Should Be Interchangeable, With Same Column Vessel, Without Any Demerits. Column ( One Number ) - Empty Column Should Be Compatible With Different Matrix Material, Ph Up To 12, Particle Size Up To 35?M And Bed Volume Up To 320Ml. Fractionation Range Of Column Matrix ( Molecular Weight Of Protein ) - Two Separate Columns ( Each One ) 1. Having Protein Separation Range Of 5-100 Kda Having Protein Separation Range Of 10-600 Kda, Fraction Collector - • Number Of Fractions Up To 30 • Vessel Type Supports Tube Sizes: 1.5 Ml / 2 Ml Microcentrifuge Tubes, 5 Ml Tubes ( 12 × 75 Mm ) , 12 Ml Tubes ( 17 × 100 Mm ) , 15 Ml Tubes ( 17 × 118 Mm ) • Fraction Volumes: 0.5 To 15 Ml , Packing & Forwarding, Insurance And Transportation Charges , Installation And Training Charges

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Name of Work: Supply, installation and commissioning of Fast Protein Liquid Chromatography (FPLC) to recover and purify collagen protein from Tannery Fleshing Waste

Sl. No.	Item Description
1	Supply, installation and commissioning of Fast Protein Liquid Chromatography (FPLC) to recover and purify collagen protein from Tannery Fleshing Waste
2	Name of the Instrument - Fast Protein Liquid Chromatography System, System configuration- Automated & Stand-alone Bench-top system for Protein purification work, Connection between PC and instrument- USB, System Capabilities - Suitable for affinity, ion exchange and Gel filtration chromatography column, Flow rate- 0.5 to 5 ml/min or wider range with automatic gradient formation capability (5 to 95% of buffer B), Pressure of the system- System should bear pressure range should between 0 to 0.5MPa along with pressure sensor, Viscosity - Sample bearing viscosity 0.6-5cP, Power supply- 100-240 VAC, 50 to 60 Hz, Power consumption - 90-100 VA, Inlet Valve - PTFE tubing, 1.5-1.8 mm, Buffer valve- PEEK tubing, 0.70-0.80 mm, Outlet and waste- ETFE tubing, 1.0-1.10 mm, Fingertight connector, 1/16”, Sample tubing - ETFE tubing, 0.70-0.80 mm, Fingertight connector, 1/16”, Pump tubing- Marprene tubing, 0.80 mm, Equipment noise level - < 60 dB A, Buffer - Alkaline and Acid mobile phase, HPLC grade water, Sample volume and Injection valve - 10ml, Rotary-type manual valve, Function Sample injection through loop, Sample Injection - Manual, Number of Injection ports - 6-8, Wavelength (nm) - Any fixed wavelength between 260-300nm, UV Flow cell - 2 mm optical path length, 2-5µl cell volume, Operating temperature:4ºC to 32ºC or wider, Software - Computer controlled system along with specific software for FPLC operation and System Control, Method Editor, Evaluation, Administration and Design runs. Along with software, License and warranty are required. Real-time process monitoring and control through touch screen display. Computer (Desktop, I5) for monitoring and data processing - Compatible with software and user friendly with fplc system control, possibly upgradable • Processor – Intel i5 10G • OS – Window / Linux / DOS • Monitor – 1 No, Size- 24 inch • Mouse-1 No, • Keyboard- 1 No, • CPU- 1 No, 8 GB RAM, 1 TB Hard disk storage, Pump type - Peristaltic pump, Single channel, four roller pump head with low pulsation, Mixer - Static mixer (0.4 ml) used for blending buffers during gradient runs. Column (one number each) - High load Columns with preparative gel filtration media also with a unique composite matrix of dextran and agarose, and a Desalting Column, the column matrix should be interchangeable, with same column vessel, without any demerits. Column (one number) - Empty Column should be compatible with different matrix material, pH up to 12, particle size up to 35µm and bed volume up to 320ml. Fractionation range of column matrix (Molecular weight of Protein) - Two separate columns (Each one) 1. Having protein Separation range of 5-100 KDa Having protein Separation range of 10-600 KDa,Fraction Collector - • Number of fractions up to 30 • Vessel type supports tube sizes: 1.5 ml/2 ml microcentrifuge tubes, 5 ml tubes (12 × 75 mm), 12 ml tubes (17 × 100 mm), 15 ml tubes (17 × 118 mm) • Fraction volumes: 0.5 to 15 ml
3	Packing & Forwarding, Insurance and Transportation charges
4	Installation and training charges