Supply Of Real-Time Pcrto The Dbt, M.Scmedicalbiotechnology Program, Dept Of Biotechnology.-Tamil Nadu

Supply Of Real-Time Pcrto The Dbt, M.Scmedicalbiotechnology Program, Dept Of Biotechnology. - Real-time PCR machine • System: should allow to use all commercially available reagents and consumables The system should be an Open system capable of running various chemistries so that Different chemistries using TaqMan, Molecular Beacon, SYBR green etc all can be performed. System should have one channel dedicated for FRET experiments without the need of a service engineer to change the filters. The system should be an Open system capable of running various chemistries so that Different chemistries using TaqMan, Molecular Beacon SYBR green etc all can be performed. The system should be capable of networking with multiple systems in the future. System should be provided with a license copy of qbase plus software from Biogazelle which is RDML compliant, and helps in Normalization, to access post –PCR Quality, helps in inter run calibration,geNorm,helps in bio-statistical analysis, CNV Analysis and MIQE Compliancy. System should be compliant with the MIQE Guidelines • Software: User friendly software shall work without PC, Software PC .Software provided with instrument should be able to perform all standard Real time PCR protocols. Software should have express load feature which allows entry of data after experiment Software should be capable to import and analyze data from any real time PCR platform and the system should be supplied with suitable computer. • Number of samples per run: Real time PCR with block of 96 x 0.2 ml tubes or plate to run typical o.2ml tubes, strips, and plates • Sample volume: 1µ1 - 50µ1 or better System should have 6 Peltier Cooling & Heating for uniform temp control Method of temperature monitoring should be calculated and Block System Should have a Detection of 2 or more different fluorescent reporters in the same tube without the use of ROX System should be capable of Detecting FAM/SybrGreen,VIC,HEX,TET,CAL Fluor Gold 540 or more. The system should have a ramp rate of: 5°C per sec or better. System should have one channel dedicated for FRET experiments without the need of a service engineer to change the filters. System should have a mass reduced block to offer better average ramp rate and 10 sec of settling time. System should have an Excitation –Emission range of 450-580nm No internal reference dye should be required. True 2 Color Multiplexing with use of 2 different fluorophores without the need of addition of any internal reference dye. System should have 3 filtered LEDs as an excitation source with 3 filtered Photodiodes for detection. • Temperature:Temperature range 0 – 100 °C with accuracy of ±0.2°C and uniformity of ± 0.4°C within 10 sec of arrival at 90°C • Gradient capability:System should have a gradient capacity with dynamic ramping. • Other technical details: System should have built in data analysis modules with advance features like well highlighting, QC flags and custom data view assist with quick analysis. System should be capable to perform Automatic allelic discrimination by end point fluorescence or threshold cycle. System should be capable to perform Gene expression analysis by relative quantity(ΔCt) or normalized expression (ΔΔCt). System should perform end point analysis for upto 2 fluorophores System should have mode for Melt curve analysis System should have Comparison of upto 5000 Ct values from different data files should be possible. System should be compatible for HRM applications. System should have the feature of Email notification with date file after run completion Warranty: Minimum Three Years of warranty

INR 1560 /-

INR 12000.0 /-

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