| 1 | 1 | Rhizobium Culture (carrier Based/ Liquid) \nA) Base: Carrier based in form of moist/ dry powder or granules, or liquid based. \nB) Viable cell count: CFU minimum 5x107 cell/g of powder, granules or carrier material of 1x108 cell/ml of liquid. \nC) Contamination level: No contamination at 105 dilutions. \nD) pH; 6.5-7.5. \nE) Particle size in case of carrier based material: All Material shall pass through 0.15- 0.212 mm IS sieve \nF) Moisture per cent by weight, maximum in case of carrier based: 30-40% \nG) Efficiency Character: Should show effective nodulation on all species listed on the packet. \nType of carrier; The carrier material such as peat, lignite, peat soil, humus, wood charcoal or similar material favouring growth of the organism.\n | | | |
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| 2 | 1.01 | Rhizobium Culture (carrier Based/ Liquid) | item1 | 1 | Nos |
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| 3 | 1.02 | Rhizobium Culture (carrier Based/ Liquid) | item2 | 1 | Nos |
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| 4 | 2 | Azotobector (carrier based /Liquid) \nA) Base: Carrier based in form of moist/ dry powder or granules, or liquid based. \nB) Viable cell count: CFU minimum 5x107 cell/g of carrier material or 1x108 cell/ml of liquid. \nC) Contamination level: No contamination at 105 dilutions. \nD) pH; 6.5-7.5. \nE) Particle size in case of carrier based material: All Material shall pass through 0.15-0.212 mm IS sieve \nF) Moisture per cent by weight, maximum in case of carrier based: 30-40% \nG) Efficiency Character: The strain should be capable of fixing at least 10mg of nitrogen per g of sucrose consumed. \nType of carrier; The carrier material such as peat, lignite, peat soil, humus, wood charcoal or similar material favouring growth of the organism.\n | | | |
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| 5 | 2.01 | Azotobector (carrier based /Liquid) | item3 | 1 | Nos |
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| 6 | 2.02 | Azotobector (carrier based /Liquid) | item4 | 1 | Nos |
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| 7 | 3 | Azospirillum (carrier based/ Liquid) \nA) Base: Carrier based in form of moist/ dry powder or granules, or liquid based. \nB) Viable cell count: CFU minimum 5x107 cell/g of powder/granules or carrier material or 1x108 cell/ml of liquid. \nC) Contamination level: No contamination at 105 dilutions. \nD) pH; 6.5-7.5. \nE) Particle size in case of carrier based material: All Material shall pass through 0.15- \n0.212 mm IS sieve \nF) Moisture per cent by weight, maximum in case of carrier based: 30-40% \nG) Efficiency Character: Formation of white pellicle in semisolid nitrogen free bromothymol blue media. \nType of carrier; The carrier material such as peat, lignite, peat soil, humus, wood charcoal or similar material favouring growth of the organism.\n | | | |
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| 8 | 3.01 | Azospirillum (carrier based/ Liquid) | item5 | 1 | Nos |
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| 9 | 3.02 | Azospirillum (carrier based/ Liquid) | item6 | 1 | Nos |
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| 10 | 4 | Phosphate Solublilzing Bacteria (P.S.B.) \nA) Base: Carrier based in form of moist/ dry powder or granules, or liquid based. \nB) Viable cell count: CFU minimum 5x107 cell/g of carrier material or 1x108 cell/ml of liquid. \nC) Contamination level: No contamination at 105 dilutions. \nD) pH; 6.5-7.5 for moist/ dry powder granulated carrier based and 5.0- 7.5 for liquid based. \nE) Particle size in case of carrier based material: All Material shall pass through 0.15- 0.121 mm IS sieve \nF) Moisture per cent by weight, maximum in case of carrier based: 30-40% \nG) Efficiency Character: The strain should have phosphate solubilizing capacity in the range of minimum 30%, when tested spectrophotometrically. \nIn terms of zone formation, minimum 5 mm solubilization zone in prescribed media having at least 3 mm thickness. \nType of carrier; The carrier material such as peat, lignite, peat soil, humus, wood charcoal or similar material favouring growth of the organism.\n | | | |
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| 11 | 4.01 | Phosphate Solublilzing Bacteria (P.S.B.) | item7 | 1 | Nos |
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| 12 | 4.02 | Phosphate Solublilzing Bacteria (P.S.B.) | item8 | 1 | Nos |
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| 13 | 5 | Mycorrhiza (Bio Fertilizers) (Liquid/Carrier based)\nA) Form/Base: Fine powder/tablets/granules/ root biomass mixed with growing substrate \nB) Particle size for carrier based: 90% should pass through 250 micron Is sieve powder formulations (60 BSS). \nC) Moisture content per cent maximum: 8- 12 \nD) pH; 6.0-7.5. \nE) total viable propagules/ gram of product: 100gm of finished product with minimum 60 spores per gram. \nF) Infectivity potential; Inoculum potential : 1200IP/g {determined by MPN method with 10 fold dilution}\n | | | |
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| 14 | 5.01 | Mycorrhiza (Bio Fertilizers) (Liquid/Carrier based) | item9 | 1 | Nos |
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| 15 | 5.02 | Mycorrhiza (Bio Fertilizers) (Liquid/Carrier based) | item10 | 1 | Nos |
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| 16 | 5.03 | Mycorrhiza (Bio Fertilizers) (Liquid/Carrier based) | item11 | 1 | Nos |
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| 17 | 6 | Potassium Mobilizing Bio- Fertilizers (KMB) \nA) Base: Carrier based in form of moist/ dry powder or granules, or liquid based. \nB) Viable cell count: CFU minimum 5x107 cell/g of powder, granules or carrier material on dry weight bsis or 1*x108cell/ml of liquid. \nC) Contamination level: No contamination at 105 dilution. \nD) pH; 6.5-7.5 carrier based in form of powder or granules and 5.0 -7.5 for liquid based. \nE) Particle size in case of carrier based moist powder: Powder shall pass through 0.15-0.212 mm IS sieve \nF) Moisture per cent by weight, maximum in case of carrier based: 30-40% \nG) Efficiency Character: minimum 10 mm solubilisation zone in prescribed media having at least 3 mm thickness. \nType of carrier; The carrier material such as peat, lignite, peat soil, humus, talc or similar material favouring growth of the microorganism.\n | | | |
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| 18 | 6.01 | Potassium Mobilizing Bio- Fertilizers (KMB) | item12 | 1 | Nos |
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| 19 | 6.02 | Potassium Mobilizing Bio- Fertilizers (KMB) | item13 | 1 | Nos |
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| 20 | 7 | Zinc Solublizing Bio-fertilizer Bacteria (ZSB) \nA) Base: Carrier based in form of moist/ dry powder or granules, or liquid based. \nB) Viable cell count: CFU minimum 5x107 cell/g of powder, granules or carrier material on dry weight basis or 1x108 cell/ml of liquid. \nC) Contamination level: No contamination at 105 dilution. \nD) pH; 6.5-7.5 for carrier based in form of powder or granules and 5.0 -7.5 for liquid based. \nE) Particle size in case of carrier based material: Powder Material shall pass through 0.15-0.212 mm IS sieve \nF) Moisture per cent by weight, maximum in case of carrier based: 30-40% \nG) Efficiency Character: Minimum 10 mm solubilization zone in prescribed media having at least 3 mm thickness\n | | | |
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| 21 | 7.01 | Zinc Solublizing Bio-fertilizer Bacteria (ZSB) | item14 | 1 | Nos |
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| 22 | 7.02 | Zinc Solublizing Bio-fertilizer Bacteria (ZSB) | item15 | 1 | Nos |
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| 23 | 8 | Carrier based NPK Consortia \nA) Base: Carrier based in form of moist powder or granules. \nB) Viable cell count: CFU minimum in a mixture of any 2 or maximum three of following microorganisms; \n1. CFU minimum Rhizobium or Azotobacter or Azospirillum (1x107per g) \n2. CFU minimum PSB (1x107per g) \n3. CFU minimum KSB (1x107per g) . \nC) Particle size in case of carrier based moist powder: All material shall pass through 0.15 to 0.212 mm IS sieve. \nD) Total viable count of all the biofertilizer organisms in the product; CFU minimum 5x107 cells per gm of carrier /powder \nE) Contamination: No contamination at 10-4 dilution for carrier based/ granule based inoculants \nF) Efficiency Character: 1) Azotobacter ( the strain should be capable of fixing at least 10 mg of Nitrogen fixation /g of C source. \n2.) Azospirillum (The strain should be capable of fixing at least 10 mg of N- fixation/g of malate applied. \n3) PSB ( minimum 5mm zone of solubilization zone on PSB media having at least 3 mm thickness. \n4) KMB (Minimum 5 mm zone of solubilization on KSB media having at least 3 mm thickness. \n5) Rhizobium ( Nodulation test positive\n | | | |
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| 24 | 8.01 | Carrier based NPK Consortia | item16 | 1 | Nos |
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| 25 | 8.02 | Carrier based NPK Consortia | item17 | 1 | Nos |
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| 26 | 9 | Liquid based NPK Consortia \nA) Individual Viable count in Liquid based: CFU minimum in a mixture of any 2 or more of following microorganisms \n1) CFU minimum Rhizobium or Azotobacter or Azospirillum (1x108 per ml). \n2) CFU Minimum PSB (1x108per ml). \n3) CFU minimum KSB (1x108per ml). \nB) Total viable count of all the bio-fertilizer organisms in the product : CFU minimum (5x108 per ml of liquid bassed). \nc) Contamination level: No contamination at any dilution. \nD) pH; 5.0 – 7.0 \nE) Efficiency Character: 1. Azotobacter ( The strain should be capable of fixing at least 10 mg N fixation /g of C- source/ 2. Azospirillum ( the strain should be capable of fixing at least 10 mg of N- fixation /g of malate applied. \n3. PSB (Minimum 5 mm zone of solubilization zone on PSB media having at least 3 mm thickness.) \n4. KMB ( Minimum 5 mm zone of solubilization on KSB media having at least 3 mm thickness.) \n5. Rhizobium (Nodulation test positive)\n | | | |
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| 27 | 9.01 | Liquid based NPK Consortia | item18 | 1 | Nos |
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| 28 | 9.02 | Liquid based NPK Consortia | item19 | 1 | Nos |
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