Supply of equipments/instruments for Department of Biochemistry, Immunology & Pathology -1.1 BIOCHEMISTRY 2.0 Vertical Biosafety Cabinet Size: 4x2x2, hepa filter of 0.2 microns, stailess steel, digital pressure monitoring sensor. 3.0 PCR Hood Size: 2x2x2, hepa filter of 0.2 microns, stainless steel, digital pressure monitoring sensor. 4.0 Micro centrifuge (Indian manufacturer) 15000RPM, capacity to handle 1.5-2ml of tubes. 5.0 Dry bath 1.5 -2ml tubes, highest range of temperature to be 100 degree celisius. 6.0 Vortex Shaker Possibly the variable speed model. 7.0 PCR Spinner 6000 RPM, for PCR (0.2ml) tubes. 8.0 Refrigerator Capable of maintaining -2 to 8 degree Celsius. 9.0 Deep freezer (340 litre) Horizontal Capable of maintaing -80 degree Celsius. 10.0 Calibrated variable micropipettes 0-10μl, 20-200μl, 100-1000μl Fully autoclavable Calibrated to the required volumes. 11.0 Sterile filter tips (aerosol free) 0.5-10μ, 2-20μl, 20-200μ, 100-1000μl. Must be sterile and must have aerosol barrier. 12.0 Micropipettes stand Preferably plastic. 13.0 PCR Optical strip tubes and caps 0.2ml. Flat cap, DNA/RNAse & Pyrogen free. 14.0 1.5ml Micro centrifuge tube DNA/RNAse & Pyrogen free. 15.0 1.5ml Micro centrifuge tube stand Preferably plastic. 16.0 Cold tube rack or cooling block or minicoller For 1.5-2ml tubes, atleast 20 slots. 17.0 Micropestle for tisuue Plastic capable of crushing tissues. 18.0 Sterile Nitrile Gloves Powder free nitrile gloves. 19.0 Facemask 2 ply (layers) 20.0 PPT kit 21.0 0.2 ml PCR tube rack Plastic 22.0 Tissue roll 23.0 Head Cap 24.0 pH Meter pH range; -2.00 to 16, pH resolution-0.001/0.01/0.1 selectable, pH calibration points- upto 6/5 point, pH calibration mode- Auto buffer recognition with option to edit values, Automatic buffer sets- USA/NIST, DIN or custom, Hold and stable indicator, Memory upto 500 data, MV, RMV Mode- Yes, ORP Mode- Yes, Push button calibration. 25.0 Electronic Balance Type/ Display- Digital /LED/LCD, Operating Temperature - 15 to 40 degree C., Power 90-250V, 50/60 Hz, 10Watt, Response time 3 sec, Accuracy- High, Readability- 0.001 mg to 1 mg, Linearity- Yes. 26.0 Spectrophotometer (Double Beam UV visible), Cell Holder-10mm 8 cell programmable holder, Optical system- Double beam, Grating 1200 lines/mm, Wavelength range-19nm-110nm, Wavelength accuracy- +/- 0.3nm, Wavelength repeatability- +/- 0.2nm, Scanning speed- Fast, Medium, Slow, Photometric accuracy- +/- 0.3% T, Photometric repeatability - +/- 0.2%T, Photometric range- 0.03-3A, 0-200%, 0-999C, Stability baseline- +/-0.001A/h @500nm, Data output port- USB, Printer port_ Parellel port, Display-320 x 240 Graphic LCD, Lamps- Deuterium Lamp & Tungsten Halogen Lamp, Detector- Silicon Photodiode, Power supply- AC 220v/50Hz. 27.0 Electrophoresis with gel caster, gel tray, COMBS & Power pack supply (Vertical) Gel size- 15.0 x 10.6 cm, Gel type- Precast or hand cast (Horizontal) Gel size- 15.0 x 10 cm. 28.0 Hot Air Oven Temperature range 50°C to 200°C, Thermometer separate, Double walled chamber, Inner of stailnless steel SS 304 grade and power coated outer surface, three heatind elements on three sides for Uniform temperature on all shelves, Air circulating fan/ air ventilation, Microprocessor based digital temperature controller with digital display, chamber size trays with hole, power 230V(AC) with all accessories. 29.0 Glucometer with strips No requirement of maitenance, Linearity - 20 to 200mg.dl, Maximum reading line of < 10 sec, Blood sample required <1.5μl, LCD display system with minimum 50 storage. 30.0 Vertical Autoclave (Pressure Steam Steriliser Aluminium Electric, Pressure Cooker type) Aluminium deep drawn seamless construction, sturdy safe, rustproof , double walled, body made of 304SS both inside and ouside, chamber size- 16 x 24, Max. pressure 15psi/ 1.5bar, Max temp-121°C, Water level indicator , automatic low water cut-off device, equipped with steam release/ control valve, high quality heat resistant backelite handles,Lid sealing by high pressure rubber gasket (radial locking with paddle lifting arrangement), Pressure gauze with clour coding to display sterilization zones graduated un Kg/cm2 and lb/inch2, Automatic pressure control switch, safety pressure release weight valve, Power supply-220V.50Hz, single phase. 31.0 Microwave Oven Capacity 30 L with lock, Over heating protection provided, Stailess steel body, Digital control system, Provided with temperature setting and defrost system. 32.0 Table Top Centrifuge Brushless Capacity 12 tubes applicable for small volume samples also, Speed range- 300 to 4500 rpm, incement 100rpm, speed accuracy ± 20rpm rotor capcity 8 x 15ml/ 12x10ml. Temperature range +3°C to 40°C, Run time 30 sec to 95 min hold (Continuous operation), Option to accelerate and decelerate the speed at various rates, Brushless DC motor & LCD display, Noise level less than 60dB. 33.0 Vaccume cleaner It should be vaccumed Cleaner with Ionizer, Powered with cutting edge Tripple I technology, Discharges negative ion in the air that traps the minute particle and clears the air of any pollutants, powered with superior dry power motor, should deliver maximum suction with superior dry power motor, Preferably metal bodied. 34.0 Refrigerator Capable of maintaining -2 to 8 degree 35.0 Induction Cook Top 2000 watt 36.0 Atomiser 37.0 Semi Automated Coagulation Analyser 1. It should be a Four Channel Coagulation Semi Automatic Analyser. 2. The channels should have 640 nm for clotting tests to remove HIL (Hemolysis, Icteric, Lipemic) interference, 405 nm for Chromatogenic tests and 800 nm for DDimer to remove interference in latex particles. 3. Principle for clotting should be scattered light detection method at 640 nm with clotting Curve being generated , displayed and printed if required. 4. It should hold 20 Reaction Cuvettes with incubation at 37°C. 5. It should have 12 Sample/ Reagent position, of which 7 numbers should be at 37°C and 5 numbers at Room Temperature. 6. One Reagent position should have magnetic Stirrer function. 7. It should have a internal Graphic Thermal Printer. 8. It should have a large Touch Screen for display of Calibration, QC data, Sample data, programming, setting and running test. 9. It should have 3 USB points, one RS232, one LAN, and power points. 10. It should be able to upgrade software with help of USB Pen Drive. 11. The Calibration should have facility to input 6 multipoint calibration data, MNPT and ISI values. 12. It should report inscends, INR, g/L, Ratio, FEU and%. 13. The settings should have option of sample ID in sequential or custom mode. 14. It should accept 14 digit sample alphaneumeric ID which can continue i n sequential mode. 15. It should perform al Clotting tests (PT, APTT, FIB, TT, Factors, LA, Protein S), Chromogenic Tests (ATIII, etc), Immunoturb tests (DDimer). 16. Company giving the system should market same branded reagents and provide technical support and consumables. 17. The system should have 12 QC options per Test, with L-J Facility, display, print, and disable/ delete points. 18. 500 nos. Single Reaction Cuvettes should be supplied as standard accessory. Quote for 100,000 Cuvettes must be given separately in the commercial bid. 19 Standard accessories should include USB Pendrive, Indian Power cord, Paper rolls, Dust cover, Reagent Holder, Stylus, magnetic beads for stirring, small reagent cups. 20. The system should accept RFID data for cuvettes loading. 21 The Test Sequence should prompt the sample / reagent name to add next in each channel, have sensors in channel for detecting sample and reagent addition and automatically do incubation, measuring and reset the channel for next test on removing the tube after results is displayed and printed. 38.0 Quote for 100,000 Cuvettes for above. 39 IMMUNOLOGY 40.0 FLUROSCENCE MICROSCOPE1. Should have high power white LED with adjustable intensity, integrated diaphragm and trinocular head.2. WLED fluorescence four filter which are BLUE,GREEN,VIOLET and ULTRA VIOLET to perform multiple application.3. Life expectancy of LED is around 30,000 hours , NO replace required.4. LED should reduces photo bleaching ,consistent intensity, quick start-up and shut down etc.5. One 40x object Flour Plan (Superior quality) and 4X /NA 0.10,10X/NA 0.25(Spring loaded),100X/NA 1.25(Oil +spring loaded) objective as infinity corrected plan achromatic objective ,each objective one in number .6. 20X AND 60X Objective should be available.7. Eye piece :two Eye piece WF 10X each F.No.22mm each .8. Should fine focus range of 2um both side fine focusing system. One side fine finger focusing.9. Should one FOG lens to reduce high intensity of fluorescence to perform some specific application where low florescence is required.10. Should have one digital 5 mega pixel camera with software to perform live imaging operation.11. Should have one FITC QC Slide for florescence .12. Should have all autoimmunity, tuberculosis, Renal and Derma Pathology, infectious disease/Antibody mark based on FIT CR / Rohadamine/Auramine 0 based application .13. As well Also validated for all bright field application.14. Complete service and application support on the installation .15. Complete training on handling the instrument, programming interpretation has to be given free of cost by the supplier.16. Boucher should be attached of the equipment and demonstration for final consideration. 41.0 Micro well Plate Reader:-ELIA (Enzme Lniked Immunosorbant Assay)1. The system should be 8-channel optical measuring system. 2. The system should be work with Touch screen and should not have a keypad. 3. It should be read U-V or flat bottom 96—well plate. 4. The photometer should be filter wheel based. 5. The system should have capacity for mono and bi-chromatic measurements. 6. The entire Micro well plate should be measured within 5 second in the monochromatic measurement mode. 7. The results i.e. Abs. Sample no. And interpretation must be seen on the screen in matrix form. Graphs should be displayed on the screen. 8. System should have lamp off mode when not in use to enhance the life of the lamp. 9. System should be provide with 405nm,450nm,492nm and 630nm standard filters. There should be at least 3 extra blank filter positions. 10. System should have facility for up to 42 user defined test protocols. 11. System should have large LCD display, with user friendly touch screen for software operation.12. System should have variable speed linear shaking facility of Micro-Well plates for removal of micro bubbles and mixing of the well solution. The time and speed should be user definable. 13. The microwell plate position should have aerosol cover facility to prevent external contaminants and stray light. 14. It should have the measurement range up to 2.5 Abs. 15. The on board software should have capability of sorting the calibration curve data for the least 8 standard in all the 42 test programs. 16. The curve should be display on screen 8x6 matrix reports must be displayed at a time. 17. The system should have up to 3 cut off equations per qualitative test. 18. It should have facility for plate mapping. Plate mapping must allow positioning of control, calibrators ,blanks and samples at any location on the plate. 19. The system must accept external dot matrix printer and must print results in preformatted matrix from giving details such as samples no. Value ,Abs and interpretation with cut off equation for qualitative results. 20. The on board software should have QCdata storage facility for up to 31 points with LEVYJENNINGCURVE. 21. 1t should be ports for external printer and transmission of data to the host computer. 22. It should have optional host computer software for extensive data management capability. ELISA Washer:- 1. The system must have 8 channels manifold and 12 channel manifold supplied with the instruments. 2. It should have a touch screen and no key pad. 3. It should have 4 bottles to it online ,one rinse ,2 wash and one waste bottle. 4. The waste bottle must have sensor. 5. The system should have 64 wash protocols.6. The system should have 10 preset for different micro plates.7. It should have two options for dispensing low and high.8. The system must offer choice to use any of the 2 wash buffers while running.9. The system must perform Tap wash, bottom wash and in case of flat wells, cross wise washing. It should have soak facility for 255s.10. The micro plate must be docked in a removable plate carrier, whose decontamination can be performed.11. The system must have Aerosol cover to prevent particulate matter during wash cycle.12. Standard accessories must contains pin for cleaning manifold,1 fuse,1 power cord. 42.0 Gel Electrophoresis:1. System should be compact semi automated bench —top analyzer.2. System be minimum capacity of 20 samples to 50 samples with simultaneous application.3. System should have hydrophobic sample applicators for precise and accurate sample application allow 100% protein applications on gels.4. System should have precise antiserum applicator for IFE ensuring complete lane coverage so as to allow full antigen and antibody interaction. 5. System should have optimized Gel Range to run various electrophoresis serum protein , protein serum protein splits Beta serum , Serum protein high resolution Alkaline and Acid, HB, Lipoprotein, Cholesterols, Alkaline Phosphatases, Ck, LD and immunofixation both serum and urine protein and Von while brand assay.6. System should have option to for user defined programming for minimum 15 methods so as to have option for additional protocol (user defined).7. System should have simple to use intuitive software.8. System should be using pre cast semi-dry buffer system as there is no need to maintained the Ph of buffer as it helps to have betterprotein separation.9. System should have continuous gel loading so as to manage high sample management with same platform.10. Gel should have pre tasted gel technology.11. System should have high voltage application for parameters like Iso electric focusing to run CSF, IgG, IEF and Hb, IEF transferring lEE or Alpha 1 Antitrypsin 1EF.12. System should have optimized temperature operation. System should have multiple ports for different strains ,wash solution and cleaning solution. 13. System should have automated cleaning protocol to attain proper daily maintenance.14. System should be working on no carryover technology. 15. System should have provide within built scanning unit and computer & laser printer system along with 2KVAonline ups with two hours backup time. 16. Starter kits and reagents, Accessories and compensation kit should be provided.software.15. System should have simple to used windows based software. 2. System should have single screen navigation. 3. System should have Gel,Sample, trace, demographics, patients, history status, attached IFES and trace analysis all visible in single window. 4. System should have option to attached IFE gel image with serum protein reports for better disease diagnosis management. 5. System should have automated Levy-Jennings analysis and standard deviations. 6.System should have automated flagging of abnormal-normal samples.Comprehensive warranty for three years that include replacement assurance on all hardware and software components of the equipment should be included in the quoted price. 43.0 MICROPROCESSOR CONTROLLED CLASS II BIO-Safety CABINET The cabinet should advanced microprocessor control, which supervise operation of all cabinet function. Temp. compensated air velocity sensors monitors both exhaust and down flow. 24 hour clock UV timer, UV run hour meter and standard.There should be programmable PIN, Which restrict unauthorized cabinet access.The biological safety cabinet should comply International Standard Certificates likes EN/NSF, ISO, JIS ETC.The cabinet should have energy efficient ECMD DC blower motor with night set back mode facility.The cabinet should have long life DUAL ,ULPA Filter for supply and exhaust (per IEST- rp-cc001.3) with 99.999% efficiency for particle size 0.1 to 0.3 microns.Should be raised armrest for elevates the operators arms to prevents inflow grille blockage for safety work.Work tray should be made of single piece stainless steel type 304,with 4B finish.Programmable automatic UV Light timer should simplify operation and extending UV Light life and saving energy.The cabinet outer surface should have antimicrobial coating for minimizing contamination .The controller should include soft touch keypad control with LCD display of airflow velocity .The cabinet should have built in warm white electronically ballasted zero flicker and instant start 5000k lighting provides excellent illumination of the work zone.The construction of cabinet should be electro galvanized steel including stand also. Internal dimension around 1275 x 550 x 670mm (wxdxh) .Inflow velocity should be around 0.45m/s.Instant start florescent lamp intensity should be around 1200 lux.The sound emission of the should be less than 63dBA. There should be UV protected sliding front sash which can be fully opened to insert and removed large instruments. The cabinet should come with following accessories .Should be UV Lamp minimum to nos electrical outlet sockets, and antimicrobial coated movable stand with wheels and brakes for easy movements.Should have,at least 15 nos user list at state/central govt. Institute in Kolkata region(List required with offer ). 44.0 Single channel variable volume micropipette :-1. Single channel variable volume ranges 10-100ul, 2-20ul, 100-1000ul, 20-200ul.2. Pipettes should have spring loaded tip cone.3. 4 digits volume display. (manual)4. Ultra light chemically resistant piston provides for overall weight reduction.5. Fully autoclave.6. Secondary adjustment easily adjust for the most accurate pipetting of different liquid without the need of fully calibration.7. Should be ce, ul, certified and NABL CERTIFICATION for pipette calibration should be provided by supplier. 45.0 WATER BATH:-Heating temp. Range - R/T -99C.Heat control - Turning knob.Set temp. Resolution - 1+k.Controller oscillation (31 water/90C) - 1+KAbsolute deviation/mean(31 water/90C)) - 2+K.Filing vol. max. - 41.Filling point min. - 60mm.Material in contact with medium-Stainless steel 1.4404. Fixed safety circuit - 109C.Weight - less than 4 kg.Permissible ambient temp. - 5-40C.Permissible relative humidity - 80%.Protection class according to DINEN 60529P21 USB INTERFACE. 46.0 ROTARY SHAKERS:- Should have 4digit LCD/LED display. Should have universal platform with rounded concern to accommodate inter changeable clamps of assorted sizes. Should have brushless induction motor with VFD speed control. Elegant plastic front. Should have variable speed 20-300rpm. Should have shaking amplitude 40mm. Should have auto start after power resumption. Volume 18 liters or more. Clamps Should be provided as per the size. 47.0 Non-refrigeration micro centrifuge:-• Non refrigerated centrifuge.• Max.Rotor Capacity 18x1.5/2.0ml vessels.• Max. speed 16,873xg (14,000rpm) for quick separation.• Rotor and lid both should be metallic and autoclaving at 121°C. • Small foot print and low access height of 23cm.• Opt bowl @design for quit operation -even without a rotor lid.• Quick lock rotor to allow for fast and ergonomic lid locking .• Soft touch one finger lid closure for ergonomic operation.• Full digital display with time and speed display.• Separate short spin key for quick centrifugation . • Automatimatic lid opening at the end of the run prevents samples warming and allows easy access to sample.• Certified aerosol tight rotor comes standard for safe centrifugation of hazardous samples. 48.0 SEMI AUTOMATE BIO- CHEMISTRY ANALYSER1. OPTICAL MODULES:- Static photometer with photometric range (340-670) 8 interference filters 340,405,450,505,546,578, 600 and 670 rim.2. Measuring Module:- Unique triple cuvette system * 18 II Flow cell3. Analysis Modes:- 1-Point linear -Rate A non linear. 2-Point linear- 1-Point sample blank linear. 1-Point non-linear -1-Point sample blank non linear. 2- Point non-linear- Absorbance. Rate A linear - Coagulation 4. Memory:- 200 User define test programs. 1000 test results. Reagent blank. 3 levels of QC.5. Results Recall:- Collated report by date ,ID, date & ID, test and date wise and patients Demographics.6. Built in QC:- Daily and monthly reporting for 3 levels LEVY —JENNING QC Graphs.7. Display High resolution Graphics LCD with back light. View area :-120 x92mm.8. Keyboard :- 41 multiplication key and 6 dynamic keys.9. Printer In built high resolution graphic thermal printer.10. Interface :- USB Connection to pc, Direct printer connectivity through USB Port, for external keyboard.11. Power:- External universal SMPS ADAPTER,18V DC,70W,115/230VAC(+10%),50/60Hz.12. Size(mm):- 205(H)X450(W)X300(D).13. Weight:- Approx 7 kgs.14. Optional accessories:- External 16 position Dry Incubator. 49 PATHOLOGY 50.0 Tissue flotation bath 51.0 Disposable Kinfe Microtome
