Supply Of Consumables And Non-Consumables -

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Supply Of Consumables And Non-Consumables -TABLE 1: Proteomics and Whole transcriptome analysis - 2D gel electrophoresis and Mass spectroscopy analysis - 1. Pre sample Processing & SDS PAGE Analysis 2. 2D Sample Analysis 13cm – pI Determination (3 - 10 or 4 – 7) 3. 2D Sample Analysis 13cm 4. Protein Identification by MALDI TOF Analysis 5. Bioinformatics Analysis including Gene Ontology - Whole transcriptome sequencing for Mycobacterium tuberculosis strains -6. Total RNA QC, Bacterial Ribodepletion and RNA sequencing library Preparation, Sequencing on Illumina or Novaseq platforms with 2x150 PE sequencing to generate 30 million reads or 3 GB/sample. Analysis: Reference-based alignment, Differential gene expression, RPKM/FPKM values and P-value significance gene list (up and down-regulated) Heat map and Clustering, Venn Diagram Annotation, GO analysis and Pathway analysis. - Table 2: Cell culture and Molecular work - 1 RPMI 1640 Medium (ATCC modification) 2 CO2 Independent Medium 3 Trypsin-EDTA 4 Fetal bovine serum 5 Gentamycin 6 Hygromycin 7 Kanamycin 8 Phorbol myristate acetate (PMA) 9 Triton-X-100 10 MTT 11 7H10 agar 12 96 well plate (only flat bottom, cell culture grade) 13 T25 flask 14 Disposable Petri dishes 15 ELISA kit for TNF-α 16 ELISA kit for IFN-γ 17 ELISA kit for IL-12 18 ELISA kit for IFN-α 19 ELISA kit for IL-10 20 ELISA kit for IL17 21 FastStart Universal Probe Master (Rox) 22 MicroAmp™ Optical 8-Tube Strip, 0.2 mL 23 MicroAmp™ Optical 8-Cap Strips 24 UNIVERSAL PROBELIBRARY PROBE 039 25 UNIVERSAL PROBELIBRARY PROBE 028