Lab Work Dna Rna Sequencing And Proteome Analysis Of Arecanut
Tender Overview
Project Description
Lab Work Dna Rna Sequencing And Proteome Analysis Of Arecanut
Corrigendum
| Sr No | Corrigendum Date | Corrigendum | Type | New Submission Date |
|---|---|---|---|---|
| 1 | 30-Jan-2020 | Extension of e tender | Date | 10-Feb-2020 |
| 2 | 10-Feb-2020 | Extension of e tender of LAB Work | Date | 20-Feb-2020 |
BOQ
| Name of Work: < QUOTATION FOR Lab Wok \"DNA/RNA SEQUENCING AND PROTEOME ANALYSIS OF ARECANUT’ > | |||||
|---|---|---|---|---|---|
| Sl.No. | Item Description | Item Code / Make | Quantity | Units | |
| 1 | 1.01 | RNA-Seq analysis from 10 tissues: \n2x150 bp PE sequencing to generate 50-60 million million reads and data analysis for differential gene expression and functional annotation (Gene Ontology and Pathway) of differential expressed genes.\n | Phase 1 | 10 | Nos |
| 2 | 1.02 | Short Reads Sequencing: Short read library 250 bp Illumina Nova Seq 6000/HiSeq X, 150 bpX2 paired end sequencing; Data: 175 Gb | Phase2 | 1 | Nos |
| 3 | 1.03 | Mate pair sequencing using library 2-4 Kb insert size Illumina Sequencing 150bp X 2; Data: 70Gb | Phase 3 | 1 | Nos |
| 4 | 1.04 | Mate pair sequencing using library 5-7 Kb insert size Illumina Sequencing 150bpX2; Data: 70Gb | Phase 4 | 1 | Nos |
| 5 | 1.05 | Mate pair sequencing using library 8-10 Kb insert size Illumina Sequencing 150bpX2; Data: 70Gb | Phase5 | 1 | Nos |
| 6 | 1.06 | 10X Chromium Library Prep with Illumina Sequencing NovaSeq6000/ HiSeq X; Data: 175Gb | Phase 6 | 1 | Nos |
| 7 | 1.07 | Oxford Nanopore Sequencing using PromethION with long reads, >15Kb Avg reads; Data: 105 Gb | Phase 7 | 1 | Nos |
| 8 | 1.08 | PacBio Sequel II Long Reads platform, Avg reads>15Kb, Data: 35Gb | Phase 8 | 1 | Nos |
| 9 | 1.09 | Proteome analysis of 10 tissues: Fractionation in solution digestion, 12 fractions for each tissue by bRPLC, each fraction to be run in technical triplicates on high resolution Orbitrap Mass Spectrometer | Phase 9 | 10 | Nos |
| 10 | 1.1 | Data analysis: \n• De novo hybrid assembly using minimum two assemblers and 3 K-mers best K-Mers. Hybrid assembly using a HPC/Cloud based platform\n• Annotation, gene prediction, phylogenetic analysis, synteny analysis, comparative genomics for phylogenomic and gene family, identification of genes involved in important biosynthetic pathways, sequence evolution of gene families\n• Integrative proteogenomics analysis and manual curation for true proteome leads to confirm accurate assembly and annotation of genome \n• Training to be provided to two Research Personnel on data analysis and briefing of analysis at the completion of each phase\n | Phase 10 | 1 | Nos |
AI Tender Summary
Tender Timeline
Tender Published
Tender notice published.
CompletedCorrigendum-1 Issued
Clarifications on tender conditions and amendments issued.
CompletedCorrigendum-2 Issued
Clarifications on tender conditions and amendments issued.
CompletedBid Submission Deadline
Online submission via eProcurement portal.
CompletedBid Opening Date
Technical bids will be opened and evaluated.
CompletedTender Documents
Download All (ZIP) ↓We takes all possible care for accurate & authentic tender information. However users are requested to refer Original source of Tender Notice / Tender Document published by Tender Issuing Agency before taking any call regarding this tender