Supply And Installation Of Lab Equipment For School Of Sciences, III Floor, MANUU Poly Bldg, Gachibowli, Hyderabad-Telangana

Supply And Installation Of Lab Equipment For School Of Sciences - 2 Specifications For Inverted Fluorescence Microscope: Na. The System Should Be Infinity-Corrected ( Rms ) Threaded Objectives With A 45 Mm Parfocal Distance Supporting Fluorescence, Brightfield, Color Brightfield, And Phase Contrast Imaging Modes, 60 Mm Lwd Condenser; 4-Position Turret With A Clear Aperture And 3 Phase Annuli. Nb. The Instrument Should Have Five-Position Chamber For 4 Fluorescence Illuminators, Led Light Source With >50, 000-Hour Life; Broad Choice Of Standard And Specialty Led Illuminators. ( List Of Led Illuminators To Be Attached ) . Nc. Imaging Methods By Single Color, Multicolor, Time Lapse, Z-Stacking, Movie Capture, Allowing To Review, Measure, And Annotate Captured Images. And Count Cells In Fluorescence Mode Post-Acquisition. Nd. System Should Have Mechanical X / Y Stage; Travel Range 120 Mm X 80 Mm With Sub Micron Resolution, Drop In Inserts To Receive Vessel And Lockdown Holders To Fix Sample In Place. List Of Available Vessel Holders ( Well Plates, Dishes, Flasks And Slides ) To Be Provided. Ne. The System Must Have Automated Focus Mechanism With Sub Micron ( 0.150 Um ) Resolution ( Single Step Accuracy ) And Mechanical Focus Wheel With Single Knob For Coarse And Fine Focus. Nf. System Must Include 5 Position Objective Turret With Front Mounted Control And Plan Flourite 10X, 20X, Objectives To Be Supplied Along With Instrument. Ng. The System Must Include 02 ( Gfp - 470 / 525 Nm And Rfp - 531 / 593 Nm ) Independent High Output Led Illuminators To Be Supplied Along With Instrument Have Independent Intensity Control. Nh. The System Must Include High-Sensitivity Monochrome Cmos - 3.2 Mp Or Better ( 2, 048 X 1, 536 ) Camera With 3.45 Μm Pixel Resolution. Ni. The System With Small Foot Print With Dimensions ( L X W X H ) - 18 X 23 X 18 Inches Preferable And Should Have Compatibility With The Onstage Incubator Nj. System Should Include Lcd Display - 18.5 Articulated Lcd Color Monitor With 1920X1080 Pixel Resolution With Embedded Pc With 4 Gb Ram. With 10Gb Ssd And 16Gb Usb 3.0 Memory Stick. N 3 Specification For Fluorimeter: Na. System Should Be Compact Fluorescence Based Quantification Of Dna / Rna / Mircorna / Primers / Ngs Library And Protein And Rna Quality ( Rin ) . Nb. System Should Have Large 5 -Inch Or Better Color Touch Screen For Easy Workflow Navigation. Nc. System Should Have The Sensitivity To Measure Picogram Concentration Of Nucleic Acids And Utilize Sample Volume As Low As 1Ul. Nd. Stores Up To 1, 000 Sample Results On-Board And More On Given Usb Stick. Ne. Compatible Plastic Ware And Standard Needs To Be Provided For The Calibration Of The System. Nf. System Should Measure The Intact Rna In Less Than 5 Seconds Per Sample. Ng. System Should Have The Below Excitation And Emission Filters Ni. Excitation - Blue 430–495 Nm, Red 600–645 Nm Nii. Emission - Green 510–580 Nm, Red 665–720 Nm. Nh. Vendor Should Support With Compatible High Sensitivity Fluorescent Dyes For Quantification Of Dna, Rna, Protein And Rna Quality Of Samples. Ni. System Should Have A On-Board Pre Programs And Reagent Calculator. Nj. System Should Have Option To Connect With Wifi For Data Transfer. N 4 Technical Specifications For Bio-Safety Unit: Na. The Bio-Safety Cabinets Should Be 4 Feet Width With Front Window Must Be A 8” Sash Opening And Ss 304 Grade Or Coated Steel Interior. Nb. The Bio Safety Cabinet Must Include Dc Motors. Nc. The Motor Must Automatically Adjust The Airflow Speed Without The Use Of A Damper To Ensure Continuous Safe Working Conditions, Even Without Maintenance Adjustments. Nd. Cabinet Must Use A Pressure Sensor ( Rather Than Anemometer ) To Detect Pressure Drop Across The Supply Filter, Rather Than In Just One Point Across The Down Flow. Ne. The Cabinet Should Display The Inflow And Down Flow Air Velocities And Must Incorporate An Led Indicator, Visual & Audible Alarm To Indicate Excessive Hepa Filters Loading, Blower Failure, Airflow Speed Failure, Incorrect Window Position. Nf. The Front Of The Cabinet Must Be Angled 10º To Help Minimize Glare. Ng. The Cabinet Noise Level Must Be Less Than 65 Db ( A ) For A 4-Foot Cabinet. Nh. Cabinet With Lights On And Fan At Operating Speed Should Consume Less Than 400 Watts Or Less Ni. The Cabinet Must Automatically Reduce Fan / Blower Motor Speed To 30% When The Front Window Sash Is In Closed Position To Ensure Reduced Energy Consumption When The Cabinet Is Not Is Use. Nj. Uv Light Must Be Programmable To Allow For Specific Exposure Times From 0 To 24 Hours. Nk. The Cabinet Should Have Provision To Fit Taps For Vacuum, Water And Noncombustible Gas. Nl. The Bio Safety Cabinet Should Be Nsf Certified With Listing On Nsf Website. Nm. The Bio Safety Cabinet Should Incorporate Hepa Filter Of The Class H 14 En 1822 Or Better And Having Minimum Efficiency Of 99.995% At 0.3 Μm Particle Size. Nn. Ventilation System Exhaust And Inflow Air Volume Approx 300-350 Cfm No. Audible And Visual Alarms For Hepa Filter Failure, Blower Failure, Airflow Speed Failure, Incorrect Window Position. Np. The Cabinet Should Be Provided With Fixed / Adjustable Height Stand, Uv Light And One Set Of Detachable Arms Rest And One / Two Electrical Outlet. Nq. The Drain Pan Of The Bsc Should Be Made Of Stainless Steel. The Drain Pan Should Not Be Painted, Or Power Coated. Nr. Port Provision For Clean And Safe Routing For Vacuum Tubing And Cables Through The Side Of The Bsc For Improved Organization And Work Efficiency. Ns. Cabinet Should Be Ce Certified And Declaration Of Moc Should Be Provided. N 5 Specifications For Micro Non Refrigerated Centrifuge: Na. Maximum Rotational Speed: 15000 Rpm Nb. Acceleration Time To Max.Rpm - 15S Nc. Braking Time From Max Rpm - 16S Nd. Noise Level - <60Db Ne. Power Supply: 230V / 50-60Hz Nf. Power Requirement: 220 W Ng. Soft Ramp - Adjustable Nfeatures: Nh. Aerosol Tight Rotor Supplied As Standard Ni. Standard Rotor 24 X 1.5 / 2.0Ml, Aerosol-Tight Lid Nj. Knob Version For Faster Setting Of Parameters Nk. Automatic Lid Opening Following Application Nsequence: Nl. Low Access Height Nm. Optibowl Design To Run The Centrifuge Without Rotor Nn. 230V / 50-60Hz N 6 Rotating Shaker: Bench Top, Digitally Controlled, Stepless Frequency Speed Control, Speed Range In Rpm- 80 To 180; Universal Platform With Adjustable Rollers And Surface To Accomodate Inter-Changable Clamps Of Assorted Sizes / Capacities For Holding Conical Flaks; Shaking Amplitude -10 Mm; Flask Sizes -50 Ml; Lcd Dsplay; Speed With Preset Facility; Timer Range In Minutes-99; Maximum Volume – 2 Liters; Motor Protection In Case Of Over Load; Power Supply- 230 V Plus Minus 10% 50 Hz; 5 Clamps For Each Of 50 Ml, 100Ml, 250 Ml, 500 Ml And 1000 Ml; With Warranty; Iso Certification Required. 7 Liquid Nitrogen Container / Canister ( Vfc ) : Na. Capacity- 6-8 Cubic Meter Capacity Nb. Cylinder Capacity- 1-3 Liters N 8 Horizontal Gel Electrophoresis Unit With Power Supply: Electrophoresis Cell Size- 20X 26 X 7.5 Cm; Gel Trays Size- 15 X 7; 7 X 10 Cm; Ready Agarose Gels Accommodation; Wide-Mini And 96 Plus Format; 8-12, 2 X 8 Well; Sample Throughput – 10-60; Base Buffer Volume- 650 Ml; Power: Maximum Power ( Watt ) - 120 Watts; Dc Current Output Rating ( Ampere ) -0 To 4; Dc Voltage Output Rating ( Volt ) 0 To 30 ; Load Regulation ( Voltage ) ± ( % Of Output + Offset ) -Less Than 0.01 +2 ( Milli Volt ) ; Load Regulation ( Current ) ± ( % Of Output + Offset ) -Less Than 0.05 + 1.5 Ma; Line Regulation ( Voltage ) ± ( % Of Output + Offset ) -Less Than 0.01 + 1 Mv; Line Regulation ( Current ) ± ( % Of Output + Offset ) -Less Than 0.05 + 0.1 Ma; Over Voltage Protection Accuracy +- 0.5 + 0.5 V; Over Voltage Protection Response Time ( Milli Sec ) -Less Than 10 Milli Second; Ripple And Noise Voltage ( 20 Hz To 20 Mhz ) Less Than 1 Mvrms Less Than 4 Mvpp; Ripple And Noise Current ( 20 Hz To 20 Mhz ) -Less Than 4 Milli Amps Rms; Readback Voltage Accuracy 0.05 + 10 Mv; Readback Current Accuracy -0.15 + 5 Ma; Read Back Voltage Resolution ( Mv ) -1; Readback Current Resolution ( Ma ) 0.1

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Name of Work: Purchase & Stores Sections – Supply and installation of LAB EQUIPMENT FOR SCHOOL OF SCIENCES
	Sl. No.	Item Description	Item Code / Make	Quantity	Units
1	1	Supply and installation of LAB EQUIPMENT
2	1.01	SPECIFICATIONS FOR INVERTED FLUORESCENCE MICROSCOPE:\na. The System should be Infinity-corrected (RMS) threaded objectives with a 45 mm parfocal distance supporting fluorescence, brightfield, color brightfield, and phase contrast imaging modes, 60 mm LWD condenser; 4-position turret with a clear aperture and 3 phase annuli.\nb. The instrument should have five-position chamber for 4 fluorescence illuminators, LED light source with >50,000-hour life; broad choice of standard and specialty LED illuminators. (List of LED illuminators to be attached).\nc. Imaging methods by Single color, multicolor, time lapse, Z-stacking, movie capture, allowing to review, measure, and annotate captured images. and count cells in fluorescence mode post-acquisition.\nd. System should have mechanical X/Y stage; travel range 120 mm x 80 mm with sub micron resolution, drop in inserts to receive vessel and lockdown holders to fix sample in place. List of available vessel holders (Well Plates, Dishes, Flasks and Slides) to be provided.\ne. The system must have automated focus mechanism with sub micron (0.150 um) resolution (single step accuracy)and mechanical focus wheel with single knob for coarse and fine focus.\nf. System must include 5 position objective turret with front mounted control and Plan Flourite 10x, 20x, objectives to be supplied along with instrument.\ng. The system must include 02 (GFP - 470/525 nm and RFP - 531/593 nm) independent high output LED illuminators to be supplied along with instrument have independent intensity control.\nh. The system must include high-sensitivity monochrome CMOS - 3.2 MP or better (2,048 x 1,536) camera with 3.45 µm pixel resolution.\ni. The system with small foot print with Dimensions (L x W x H) - 18 x 23 x 18 inches preferable and should have compatibility with the onstage Incubator\nj. System should include LCD Display - 18.5" articulated LCD color monitor with 1920x1080 pixel resolution with Embedded PC with 4 GB RAM. With 10GB SSD and 16GB USB 3.0 memory stick. \n	Item1	1	Nos
3	1.02	SPECIFICATION FOR FLUORIMETER:\na. System should be compact fluorescence based quantification of DNA/RNA/ mircoRNA/ primers/NGS library and Protein and RNA Quality (RIN).\nb. System should have Large 5 -inch or better color touch screen for easy workflow navigation.\nc. System should have the sensitivity to measure picogram concentration of nucleic acids and utilize sample volume as low as 1ul.\nd. Stores up to 1,000 sample results on-board and more on given USB stick.\ne. Compatible plastic ware and standard needs to be provided for the calibration of the system.\nf. System should measure the intact RNA in less than 5 seconds per sample.\ng. System should have the below excitation and emission filters\ni. Excitation - Blue 430–495 nm, Red 600–645 nm\nii. Emission - Green 510–580 nm, Red 665–720 nm.\nh. Vendor should support with compatible high sensitivity fluorescent dyes for quantification of DNA, RNA, Protein and RNA quality of samples.\ni. System should have a On-board pre programs and reagent calculator.\nj. System should have option to connect with WiFi for data transfer.\n	Item2	1	Nos
4	1.03	TECHNICAL SPECIFICATIONS FOR BIO-SAFETY UNIT:\na. The Bio-safety cabinets should be 4 feet width with front window must be a 8” sash opening and SS 304 grade or coated steel interior.\nb. The Bio Safety Cabinet must include DC motors. \nc. The motor must automatically adjust the airflow speed without the use of a damper to ensure continuous safe working conditions, even without maintenance adjustments.\nd. Cabinet must use a pressure sensor (rather than anemometer) to detect pressure drop across the supply filter, rather than in just one point across the down flow.\ne. The cabinet should display the inflow and down flow air velocities and must incorporate an LED Indicator, visual & audible alarm to indicate excessive HEPA filters loading, blower failure, airflow speed failure, Incorrect window position.\nf. The front of the cabinet must be angled 10º to help minimize glare. \ng. The cabinet noise level must be less than 65 dB(A) for a 4-foot cabinet.\nh. Cabinet with lights on and fan at operating speed should consume less than 400 watts or less \ni. The cabinet must automatically reduce fan/blower motor speed to 30% when the front window sash is in closed position to ensure reduced energy consumption when the cabinet is not is use.\nj. UV light must be programmable to allow for specific exposure times from 0 to 24 hours.\nk. The Cabinet should have provision to fit taps for Vacuum, Water and Noncombustible gas.\nl. The Bio safety Cabinet should be NSF certified with listing on NSF website.\nm. The Bio safety cabinet should incorporate HEPA filter of the class H 14 EN 1822 or better and having minimum efficiency of 99.995% at 0.3 µm particle size.\nn. Ventilation System Exhaust and Inflow air volume approx 300-350 CFM \no. Audible and visual Alarms for HEPA filter failure, blower failure, airflow speed failure, Incorrect window position.\np. The cabinet should be provided with fixed / adjustable Height Stand, UV Light and one set of detachable arms rest and one / two electrical outlet.\nq. The Drain Pan of the BSC should be made of Stainless Steel. The drain pan should not be painted, or power coated. \nr. Port provision for clean and safe routing for vacuum tubing and cables through the side of the BSC for improved organization and work efficiency.\ns. Cabinet should be CE certified and declaration of MOC should be provided.\n	Item3	1	Nos
5	1.04	SPECIFICATIONS FOR MICRO NON REFRIGERATED CENTRIFUGE:\na. Maximum Rotational Speed: 15000 rpm\nb. Acceleration time to max.rpm - 15s\nc. Braking time from max rpm - 16s\nd. Noise level - <60dB\ne. Power supply: 230V/50-60Hz\nf. Power requirement: 220 W\ng. Soft ramp - adjustable\nFeatures:\nh. Aerosol tight rotor supplied as standard\ni. Standard rotor 24 x 1.5/2.0ml, aerosol-tight lid\nj. Knob version for faster setting of parameters\nk. Automatic lid opening following application\nSequence:\nl. Low access height\nm. Optibowl design to run the centrifuge without rotor\nn. 230V/50-60Hz\n	Item4	1	Nos
6	1.05	ROTATING SHAKER: Bench Top, Digitally controlled, Stepless frequency speed control, Speed range in RPM- 80 to 180; Universal platform with adjustable rollers and surface to accomodate inter-changable clamps of assorted sizes/capacities for holding conical flaks; Shaking amplitude -10 mm; Flask sizes -50 ml; LCD dsplay; Speed with preset facility; Timer range in minutes-99; Maximum volume – 2 liters; Motor protection in case of over load; Power supply- 230 v plus minus 10% 50 hz; 5 clamps for each of 50 ml, 100ml, 250 ml, 500 ml and 1000 ml; with Warranty; ISO certification required.	Item5	1	Nos
7	1.06	LIQUID NITROGEN CONTAINER/CANISTER (VFC): \na. Capacity- 6-8 cubic meter capacity \nb. Cylinder capacity- 1-3 liters\n	Item6	3	Nos
8	1.07	HORIZONTAL GEL ELECTROPHORESIS UNIT WITH POWER SUPPLY: Electrophoresis Cell size- 20x 26 x 7.5 cm; Gel trays size- 15 x 7; 7 x 10 cm; Ready agarose gels accommodation; Wide-mini and 96 plus format; 8-12, 2 x 8 well; Sample throughput – 10-60; Base buffer volume- 650 ml; Power: Maximum Power (Watt) - 120 watts; DC Current Output Rating (Ampere)-0 to 4; DC Voltage Output Rating (Volt) 0 to 30 ; load Regulation (Voltage) ± (% of output + offset)-less than 0.01 +2 (milli volt); Load Regulation (Current) ± (% of output + offset) -less than 0.05 + 1.5 mA; Line Regulation (Voltage) ± (% of output + offset) -less than 0.01 + 1 mV; Line Regulation (Current) ± (% of output + offset) -less than 0.05 + 0.1 mA; Over Voltage Protection Accuracy +- 0.5 + 0.5 V; Over Voltage Protection Response Time (milli sec) -less than 10 milli second; Ripple and Noise Voltage (20 Hz to 20 MHz) less than 1 mVrms less than 4 mVpp; Ripple and Noise Current (20 Hz to 20 MHz) -less than 4 milli amps rms; Readback Voltage Accuracy 0.05 + 10 mV; Readback Current Accuracy -0.15 + 5 mA; Read back Voltage Resolution (mV) -1; Readback Current Resolution (mA) 0.1	Item7	1	Nos