Services Provided By Medical Laboratories - Gsi For A Single Cell Sequencing Service. In The Project Creation, Complex Development (Rrf-2.3.1-2022-00003), A Single Cell 3 Transcriptomic Sequencing And Gene Expression Analysis Of 16 Samples, Which Is 16 Cell Suspensions To Write A Complete Expressed Mrna Set To Cdns, It Means Software Processing. 3 Steps In The Process: • Accurately Sorting The Cells In The Test Sample By Barcoding, Where The Barcode Allows For Unique Identification And Tracking Of The Cells. • Cdns Synthesis From The Total Transcript Of 3 (Total-Rns Set In The Cell), With A Widely Used Technique In The Field Of Science. This Step Involves Creating A Cdns Directory. • Sequencing Cdns And Creating Quality-Controlled Score Files. As A Result Of The Three Processes And The Barcodes System, The Total Active Gene Stock Of The Unique Cells Tested Can Be Examined Through Mrna Expression And The Results Can Be Compared To Other Results Of The Discipline. • The Experimental Arrangement Is Also Suitable For A Single Cell Examination Of Human And Mouse Cells. • The Single Cell Separation Is Carried Out By A Specialist Or Under The Strict Supervision Of Such A Specialist, On A Suitable Device That May Be With The Tenderer Or Contracting Authority. • The Cost Of Transporting The Samples To The Tenderer, Or In The Opposite, Is The Cost Of The Tenderer And The Equipment Required For The One -Cell Separation To Be Transported By The Tenderer. • Further Steps After Separation Are Taken By The Tenderer. Providing The It And Technical Infrastructure Necessary For Performance Is The Task Of The Successful Tenderer. • After The Entire Processing Process, The Remainder Of The Samples Must Be Destroyed By The Tenderer In Accordance With The Applicable Legal Requirements. • The Tenderer Undertakes The Initial Steps Of Software Processing The Results. These Are: Read Mapping, Quality Control, Gene Expression Quantification, Which Results In A Quality -Controlled Gene Expression Matrix That The Contracting Authority Can Use In Its Further Work. • The Tenderer Will Hand Over The Entire Sequencing Results Created At The End Of The 3 -Step Process Or Makes Available To The Contracting Authority For Further Processing. Including Raw Fastq Files And Gene Expression Matrices (Expression Data Per Cell). • Single Cell Cultures Are Produced By Droplet/Microfluidics Technique Or The Same Reliability Used In The Literature, Which Ensures Comparability Of Experimental Results With International Results. • Sample Indexing And Library Making Provides Fastq Data Output And Cell/Gene Matrix, Or Other Data Outputs With The Same Information Containing Equivalent Software. The Contracting Authority Under The Contracting Contract 16 Samples Of Single Cell 3 Transcriptom Sequencing And Genexpr
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