Tenders Are Invited For Laboratory, Optical And Precision Equipments (Excl. Glasses) – Acquisition Of A Microscope In The Digitall Project

Laboratory, Optical And Precision Equipments (Excl. Glasses) – Acquisition Of A Microscope In The Digitall Project. 1 New Two-Photon Microscope, With The Following Minimum Technical Parameters: In Materials Science And Biology Samples, With Low-Energy Excitation, With The Help Of The Local Light Emission Of The Objects To Be Examined, The Equipment Is Capable Of Complete Imaging With A Micrometer Resolution And A Range Of 1 Mm2. Special Two-Photon Excitation Is Achieved With The Help Of The High Intensity Of The Laser Excitation And Its Low-Energy Photons. Adequate Space And Time Resolution Of The Emission Following Two-Photon Excitation Results In A 3D Image With Adequate Resolution. 1. New Device 2. The System Should Simultaneously Enable: O Freely Controllable Slow (Galvo-Galvo) Or Full-Frame Fast (Galvo-Resonant) Scanning With A Tunable Laser Beam At Least Between 700-1100 Nm O Galvo-Galvo Or Galvo - Resonant Scanning At 1040 Nm With A Fixed Laser Beam 3. The System Should Enable Both In Vitro And In Vivo Experiments And Switching Between The Two Modes. 4. The Microscope Should Provide A Camera Image In Addition To Detection With Photon Multiplier Tubes (Pmt), And The Device Should Be Able To Project Images Obtained By Two Methods Onto Each Other. Minimum Camera Parameters: O Sensor Type: Complementary Metal-Oxide Semiconductor (Cmos) O Resolution: At Least 3 Megapixels O Pixel Size: At Least 3.45 Μm X 3.45 Μm O Imaging Speed: At Least 40 Fps O Optics: At Least 1/1.8 Inch 5. The Device Must Have At Least 2 Upper And At Least 2 Lower Gated Detectors With A Quantum Efficiency Of At Least 40%, Which Are Not Damaged Even During Photostimulation. The Configuration Of The Device Should Allow The Expansion Of At Least 1 Additional Upper Gaasp (Gallium Arsenide Phosphide) Detector. 6. The Dichroic Mirror/Filter Combination Between The Detectors Should Be Replaceable. 7. The System Should Be Calibrated With At Least 16X (0.8 Numerical Aperture, 3.0 Mm Working Distance) And 20X (1.0 Numerical Aperture, 2.0 Mm Working Distance) Objectives, Which Can Be Freely Changed. 8. The Device Must Have: O The Ability To Move The Lenses In The Z Direction/Depth: In A Range Of Movement Of At Least 50 Mm (Coarse Depth Movement), In A Range Of Movement Of 400 Μm Using A Piezoelectric Actuator (Fast Fine Movement). O With An Object Moving Table With The Possibility Of Lateral (Xy) Movement: At Least In The Range Of +/- 25 Mm, In The X / Y Direction With The Object Moving Table, The Sample Can Be Positioned Under The Objective With An Accuracy Of At Least 1 Μm. 9. With The 16X Magnification Lens, The Size Of The Field Of View (Ie Fov) Should Be At Least 300X300 Μm. 10. The Pixel Dwell Time Must Be Variable. 11. The System Should Be Able To Perform Automated Scanning Protocols. 12. It Is Not Necessary To Provide Software To Evaluate The Images, But For Further Processing, The Measurement Files Should Be Available: .Tiff, .Multi-Tiff, .Mp4, And .Hdf5 Image File Formats. 13. At Least 8-Bit Electronics Capable Of Controlling At Least 4 High-Performance I/O Devices. 14. The Microscope Pane

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