Tenders Are Invited For Laboratory, Optical And Precision Equipments (Excl. Glasses) – A Label-Free 3D Quantitative Imaging System Of Living Cells

Laboratory, Optical And Precision Equipments (Excl. Glasses) – A Label-Free 3D Quantitative Imaging System Of Living Cells. Epfl Plans To Acquire A Label-Free 3D Quantitative Imaging System For Living Cells (Holotomography, Or Equivalent Diffraction/Interferometry Optical Tomography Technology), Equipped With An Integrated Multichannel Fluorescence Module, Intended For Its Mechanics Of Soft And Biological Matter Laboratory (Mesobio) At The School Of Engineering (Sti). The Equipment Will Be Dedicated To Cutting-Edge Research Requiring Quantitative, Non-Invasive And Three-Dimensional Observation Of Living Cells Over Time, Correlated With Fluorescent Marking Of Specific Cellular Structures. Taking Into Account The Needs Of Epfl And The Specific Scientific Objectives Of Its Mesobio Laboratory, Emphasis Will Be Placed On The Following Areas Of Research: Quantitative And Correlative Imaging Of Living Cells, Combining Label-Free 3D Tomography Based On The Refractive Index And Targeted Fluorescence Microscopy, In Particular For: • Long-Term Quantitative Monitoring Of The Morphology Of Living Cells, Their Dry Mass And The Dynamics Of Their Volume, Without Fixation Or Labeling, • Imaging Correlative Multimodality Combining Label-Free 3D Tomography And Fluorescent Labeling Of Specific Organelles Or Molecular Targets, • The Study Of Non-Equilibrium Dynamics And Phase Transitions In Living Matter By Means Of Quantitative Measurements Of Structure And Dynamics At The Cellular And Subcellular Scales.