Tenders Are Invited For Real-Time Polymerase Chain Reaction (Pcr) Equipment – Hochschule Reutlingen - Real-Time-Pcr-System (Qpcr-System)

Real-Time Polymerase Chain Reaction (Pcr) Equipment – Hochschule Reutlingen - Real-Time-Pcr-System (Qpcr-System). 1. Object Of Procurement A Plate-Based Qpcr System With At Least 96 Reaction Positions For The Quantitative Analysis Of Nucleic Acids Is To Be Procured. Systems In 96-Well Or 384-Well Formats Are Acceptable; A 384-Well Format Is Not A Minimum Requirement. 2. Area Of ​​Application The System Is Used For Molecular Biology Applications, In Particular For The Analysis Of Gene Expression, For The Quantification Of Nucleic Acids And For Standardized Qpcr-Based Examinations In The Field Of Biomedical Research And Genomics. 3. Functional Requirements The System Must Be Particularly Suitable For: - Real-Time Pcr Measurements - Quantitative Gene Expression Analyzes (Rt-Qpcr) - Absolute And Relative Quantification Of Nucleic Acids - Melting Curve Analyzes - Multiplex Analyzes - Genotyping And Copy Number Variation (Cnv) Applications - Evaluation And Documentation Of The Measurement Results 4. Basic Technical Requirements A System With The Following Basic Properties Is Required: - Thermocycler Unit Suitable For The Intended Qpcr Applications - At Least 96 Reaction Positions Per Run; 96-Well And 384-Well Systems Are Permitted - Suitable Optical Detection System - Manufacturer-Validated Reaction Volume Range That Includes Reaction Volumes Of 10 Μl Or Less - Temperature Range At Least 4-99 °C; Temperature Accuracy And Uniformity At Most +/-0.5 °C Or Better - Maximum Ramp Rate At Least 2.5 °C/S - Fluorescence Detection With At Least Five Independently Usable Optical Channels Or Multiplex Detection Of At Least Five Targets Per Well - Support Of Intercalating Dye Chemistries (E.G. Sybr Green) And Hydrolysis Probes - Quantitative Linear Dynamic Range Of At Least 6 Log10 Levels - Standard Melting Curve Analysis - Hardware And Software For Measurement Control And Standard Data Analysis Including Cq/Ct Determination, Standard Curves As Well As Absolute And Relative Quantification - Export Of Measurement And Evaluation Data In Common Further Processable Formats - Regular Operation, Data Storage And Standard Data Analysis Without A Mandatory External Cloud Service Or Ongoing Cloud Subscription - Complete Suitability For Safe Routine Operation In University Research Laboratories 5. Scope Of Services The Scope Of Services Includes In Particular: - Free Delivery Point Of Use - Installation And Commissioning - Instruction And Training - Provision Of The Required Software Named In The List Of Services - Complete Technical Documentation - Ready-To-Use Handover 6. Special Information - Requirements For Calibration, Maintenance, Service, Software Maintenance And Required Consumables Must Be Stated In The Offer In Accordance With The Tender Documents. - On-Site Requirements Must Be Stated In Full.