Tenders Are Invited For Microscopes – Procurement Of A Cryo-Confocal Fluorescence Microscope

Microscopes – Procurement Of A Cryo-Confocal Fluorescence Microscope. The Ibmm Would Like To Purchase A Brand New Cryo-Confocal Fluorescence Microscope (Cryo-Kfm), Which Will Be Integrated Into A Cryo-Clem Workflow For Cellular Cryo-Et And In Situ Structural Biology. The Cryo-Kfm Should Be Equipped With At Least 4 Fluorescence Channels (Lasers) And Have A Highly Sensitive, High-Resolution Detector System For Confocal Microscopy. The Microscope System Should Also Allow Switching Between Confocal And Widefield Fluorescence Microscopy By Being Equipped With Appropriate Light Sources, Filters And A Camera. It Should Also Be Equipped With At Least One Cryo-Compatible Air Lens With The Highest Possible Numerical Aperture And A Magnification Of At Least 50X. Furthermore, The Cryo-Kfm Should Have A Cryo-Stage, Which Allows The Samples (Cryo-Em Grids With Vitrified Cells) To Be Loaded, Imaged And Unloaded At < -150°C, Without The Samples Ever Being Exposed To A Higher Temperature And Without The Samples Being Significantly Contaminated By Condensing Humidity. The Cryo Stage Must Be Compatible With Common Cryo-Em Grids And So-Called Autogrids For The Further Cryo-Clem Steps. The Motorized Cryo-Stage Should Be Integrated Into The User Interface Of The Software For Operating The Cryo-Kfm. This User Interface Should Be As Simple And Efficient As Possible To Use; The Entire Microscope System Should Have Complete, Ready-To-Use Hardware And Software. Particularly Important Are High Imaging Quality, Robustness In Recovering The Imaged Samples Without Loss Of Quality For The Next Cryo-Clem Steps (Cryo-Fib Milling And Cryo-Et), And User-Friendliness.