Tenders Are Invited For Laboratory, Optical And Precision Equipments (Excl. Glasses) – Delivery Of A System For Sequencing Nucleic Acids Using Long-Read Technology; Enabling The Analysis Of The Structure And Function Of A Plant Holobiont - Acronym [Sequenator] For The Institute Of Agrophysics. B. Dobrzański Pan In Lublin

Laboratory, Optical And Precision Equipments (Excl. Glasses) – Delivery Of A System For Sequencing Nucleic Acids Using Long-Read Technology; Enabling The Analysis Of The Structure And Function Of A Plant Holobiont - Acronym [Sequenator] For The Institute Of Agrophysics. B. Dobrzański Pan In Lublin. The Subject Of The Order Is The Supply Of A Nucleic Acid Sequencing System Using Long-Read Technology; Enabling The Analysis Of The Structure And Function Of A Plant Holobiont - Acronym [Sequenator] For The Institute Of Agrophysics. B. Dobrzański Pan In Lublin. The Scope Of The Order Includes The Delivery To The Ordering Party Of: A System For Sequencing Nucleic Acids Using Long-Read Technology; Enabling The Analysis Of The Structure And Function Of A Plant Holobiont - Acronym [Sequenator]. A Detailed Description Of The Subject Of The Order Is Included In Annex No. 1 To The Swz. List Of Minimum Required Technical And Utility Parameters System For Sequencing Nucleic Acids Using Long-Read Technology; Enabling The Analysis Of The Structure And Function Of A Plant Holobiont - Acronym [Sequenator] No. Minimum Required Parameter: Condition: Offered Parameters/Conditions/Model (To Be Completed By The Contractor): 1. System For Sequencing Nucleic Acids Using Long-Read Technology; Enabling The Analysis Of The Structure And Functions Of A Plant Holobiont - Acronym [Sequenator] Necessary Condition 2. Weight Of The Device Not More Than 360 Kg Necessary Condition 3. Working Area Occupied By The Device Not More Than 0.6 M2 Necessary Condition 4. Dimensions Of The Device Not More Than: Height 170 Cm, Width 95 Cm, Depth 90 Cm Necessary Condition 5. Device Equipped With An Lcd Display With A Touch Panel Used For Interactive Operation By The User. A Necessary Condition 6. A Working Space Of The Device Equipped With: A Robotic Arm, A Space For Reagent Plates, A Space For Containers With Tips, A Space For Sequencing Plates (Chips), A Waste Discharge Zone. A Necessary Condition 7. A Device Enabling Real-Time Sequencing Of Single Dna Molecules. A Necessary Condition 8. A Device Enabling The Possibility Of Obtaining >5Gb Of Data And >370,000 Readings From One Chip, >80Gb Of Data And >6,000,000 Reads At Full Device Throughput Necessary Condition 9. Dna Sequencing Using Deoxyribonucleotide Triphosphates Covalently Linked To Appropriate Fluorophores - Fluorophores Attached To The 5 Terminal Phosphate Residue (Phosphate In The Gamma Position) Necessary Condition 10. The Sequencing Reaction Takes Place In Reaction Wells In Which A Single Recombinant Dna Polymerase Molecule And Dna/Cdna Template Necessary Condition 11. Real-Time Sequencing Reactions Of Single Dna Molecules Take Place In A Volume Of The Order Of Zeptolitres Necessary Condition 12. Genomic Dna Sequencing Without The Need For Prior Amplification Of Biological Material By The Polymerase Chain Reaction - Pcr Necessary Condition 13. Device Enabling Sequencing Of Full-Length Transcripts After Prior Conversion Of Rna Material To Complementary Dna (Cdna) Using The Reverse Transfer Method

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