Quotation are invited for Reagents, Consumables, Equipment for Human and Animal Laboratories, and Medical Products 1 Azide Agar for Erysipelothrix rhusiopathiae isolation, 500g "Blood Agar culture media (5% sheep or horse blood). Culture medium incubated at 37°C in an atmosphere enriched with 5% sheep or horse blood. Selective medium for isolating Erysipelothrix rhusiopathiae colonies (Azide Blood Agar). Culture medium formulated to isolate Erysipelothrix rhusiopathiae while inhibiting unwanted bacteria. Composition: . Tryptone: 18.0 g/L (nitrogen and vitamins source) . Beef extract: 3.0 g/L (nutrients) . Sodium chloride (NaCl): 5.0 g/L (osmotic balance) . Sodium azide (NaN): Gramnegative inhibitor (0.010.05%) . Agar: solidifying agent . Sheep or horse blood: hemolysis detection ( or hemolysis by E. rhusiopathiae) . Gentamicin: increases selectivity by inhibiting unwanted bacteria" 2 Baird-Parker Agar base, 500g "Selective medium used with supplements for the isolation and enumeration of Staphylococcus aureus in food, food products, and water. Composition: . Casein peptone: 10.0 g . Meat extract: 5.0 g . Yeast extract: 1.0 g . Glycine: 12.0 g . Sodium pyruvate: 10.0 g . Lithium chloride: 5.0 g . Agar: 15.020.0 g" 3 Mac Conkey, Agar, 500g "Lyophilized culture medium for the isolation and identification of coliforms. Composition: . HN extract: 3 g . Gelatin peptone: 17 g . Lactose: 10 g . Bile salts: 1.5 g . Sodium chloride: 5 g . Crystal violet: 0.001 g . Neutral red: 0.03 g . Agar: 13.5 g" 4 Pleuropneumonia-Like Organisms medium ( PPLO), 500g "Supplied with additives for mycoplasma growth: . Horse or calf serum: 1020% (sterol source, essential) . Yeast extract: 510% . Glucose: 0.51% (energy source) *Phenol red: pH indicator (optional) . Antibiotics (e.g., penicillin, amphotericin B, thallium acetate): to inhibit cellwall bacteria Typical composition (per liter) powder base: . Pancreatic digest of casein: 20 g . Peptic digest of animal tissue: 17.5 g . Sodium chloride (NaCl): 5 g . Disodium phosphate (NaHPO): 5 g . Agar: 15 g (if solid medium) . Final pH: 7.8 ± 0.2 at 25°C " 5 Muller Hinton, 500g "MuellerHinton Agar is a standardized medium recommended for antibiotic susceptibility testing of nonfastidious bacteria. Composition (MH): . Acid hydrolysate of casein (peptone): 17.5 g . Meat extract: 2.0 g . Starch: 1.5 g . Calcium: 2025 mg . Magnesium: 1012.5 mg . Agar: 15.0 g" 6 Chromogenic Listeria Agar, 500g "Chromogenic Listeria Agar is a highly selective and differential medium formulated according to the Ottaviani & Agosti (ALOA) principle for the detection of Listeria monocytogenes and other Listeria species. Selectivity is achieved through lithium chloride and antimicrobial agents, while differentiation is based on enzymatic chromogenic substrates and phospholipase activity. Typical composition (per liter): . Peptones: nutrient source . Lithium chloride: inhibits competing bacteria . Selective antibiotics: e.g., ceftazidime, nalidixic acid, cycloheximide . Chromogenic substrate: detects phosphatidylinositol phospholipase C (PIPLC), specific to L. monocytogenes . Esculin + ferric ammonium citrate: for hydrolysis detection (black halo) . Agar: 1215 g . Final pH: 7.2 ± 0.2 at 25°C " 7 Real-time PCR kits for Rickettsia/universal detection " Readytouse complete kits capable of detecting bacterial variants. Kits supplied with internal extraction controls (IEC) and positive nucleic acid controls specific to the bacteria. Minimum 100 reactions per kit, compatible with FAM, VIC, and ROX detection channels. Kits provided with usage protocols." 8 Real-time PCR kits for Rift Valley Fever " Readytouse complete kits capable of detecting Rift Valley fever virus. Complete kits supplied with internal extraction controls (IEC) and positive nucleic acid controls for Rift Valley fever virus. Kits containing at least 100 reactions per kit, with detection channels compatible with FAM, VIC, and ROX reading channels. Kits are provided with usage protocols." 9 Real-time PCR kits for CrimeanCongo Hemorrhagic Fever "Complete realtime PCR (qPCR) kit for detection of CrimeanCongo Hemorrhagic Fever virus (CCHFV). Readytouse kits including internal extraction controls (IEC) and positive CCHFV nucleicacid controls. Minimum 100 reactions per kit. Detection channels compatible with FAM, VIC, and ROX. Kits supplied with user protocols. " 10 Real-time PCR kits for mpox "Complete realtime PCR (qPCR) kits for detection of Mpox virus Internal Controls (Extraction + Amplification Controls): . Kits include internal extraction controls (IEC) such as RNase P, ensuring sample integrity, extraction efficiency, and PCR inhibition monitoring. . Internal positive controls (IPC) premixed in master mixes help validate amplification performance. Supplied with positive Mpox virus nucleicacid controls Minimum 100 reactions per kit Detection channels compatible with FAM, VIC, and ROX Kits provided with user protocols " 11 "- PCR Master Mix (optimized for Orthopoxvirus DNA amplification) - Pathogen-specific primers and probes - Internal Control reagents - Positive and negative controls (for assay validation) - Instructions for use (IFU) " 12 RNA extraction kits "Universal RNA extraction kit for total blood, animal tissues/organs, and swabs RNeasy Mini Kit, 250 reactions Sample range: 0.5 to 30 mg Elution volume: 30 to 100 µL Complete kit, 250 reactions Storage: Room temperature (1530 °C)" 13 DNA extraction kits "Universal DNA extraction kit for total blood, animal tissues/organs, and swabs DNeasy Mini Kit, 250 reactions Sample range: 0.5 to 30 mg Elution volume: 30 to 100 µL Complete kit, 250 reactions Storage: Room temperature (1530 °C)" 14 Spectral calibration kit for realtime PCR systems "Complete spectral calibration kit for the Applied Biosystems StepOne realtime PCR system (96well) For use with realtime PCR (qPCR) applications Kit includes: One background plate, Two 96well fast reaction plates containing spectral dyes (Plates I and II) Plates I and II contain calibration dyes compatible with FAM, VIC, and ROX detection channels Kit supports fluorescence detection calibration Spectral calibration kit type Storage: Protect from light Supplied with user manual/protocol for calibration procedures " 15 Calibration kit for an Applied Biosystems 9700 thermal cycler "Calibration/verification kit for the Applied Biosystems GeneAmp PCR System 9700 thermocycler Used to check and validate amplification performance before use Ensures reliability of temperature cycling for molecular diagnostics Includes qualitative controls (QI/QO) to verify temperaturecycle accuracy Designed for use with the GeneAmp 9700, 96well format Intended for performance verification and calibration of the thermocycler " 16 Calibration kit for a Nexus thermal cycler "Calibration/verification kit for a Nexus thermocycler Used to check and validate amplification performance prior to use Ensures reliable temperature cycling for molecular diagnostic applications Includes qualitative controls (QI/QO) to verify temperaturecycle accuracy Designed for use with the Nexus PCR system, 96well format Intended for performance verification and calibration of the thermocycler " 17 Positive controls for realtime PCR use "Kits including primers and probes for internal process controls (IPC) to confirm that DNA/RNA has not been degraded or inhibited during purification Kits also include positive qPCR amplification controls for each of the following pathogens: Brucellosis, Rift Valley Fever, FootandMouth Disease, Lumpy Skin Disease, Toxoplasmosis, CrimeanCongo Hemorrhagic Fever, Rickettsia, Mpox." 18 Positive controls for conventional PCR "Kits including primers and probes for Internal Process Controls (IPC) to confirm that DNA/RNA has not been degraded or inhibited during purification Kits also include positive controls for conventional PCR amplification Positive controls provided for each of the following pathogens: Brucellosis, Rift Valley Fever, FootandMouth Disease, Lumpy Skin Disease, Toxoplasmosis, CrimeanCongo Hemorrhagic Fever, Rickettsia, Mpox." 19 Reference bacterial strains Erysipelothrix rhusiopathiae ATCC 19414 20 Ivermectine(pharmaceutical reference standard) ". Ivermectin is a white to offwhite crystalline powder. . Insoluble in water . Soluble in: methanol, ethanol, propylene glycol . Purity: 99.0%" 21 Dovinix (pharmaceutical reference standard) "Dovinix is a white to offwhite crystalline powder. Slightly soluble in water More soluble in polar organic solvents Molecular formula: CHINO Molar mass: 266.64 g/mol Purity: 99.0%" 22 oxytetracycline (pharmaceutical reference standard) "Oxytetracycline is a yellow crystalline powder Molar mass: 460.4 g/mol Melting point: 182°C Solubility: . In its nonionized form, it is poorly soluble in water . In its ionized form, it is soluble in water and in alcohols . Insoluble in most organic solvents Purity: 99.0%" 23 Novalgine/Dipyrone (pharmaceutical reference standard) "Novalgine is a white crystalline powder with a faint aromatic odor. Molecular formula: CHNNaOS · HO Molecular weight: ~351.3351.4 g/mol Solubility: . Soluble in hot water . Slightly soluble in cold water . Soluble in ethanol Purity: 99.0% " 24 Albendazole (pharmaceutical reference standard) "Albendazole Physical appearance: white to offwhite or slightly yellowish crystalline powder Odor: odorless, polymorphic Solubility: . Practically insoluble in water . Very slightly soluble in ethanol . Soluble in anhydrous formic acid Molecular formula: CHNOS Molar mass: 265.33 g/mol Purity: 99.0% " 25 Dexamethasone(pharmaceutical reference standard) "Dexamethasone is a white, odorless crystalline powder Molecular formula: CHFO Molar mass: ~392.47 Tender Link : http://solicitations.fhi360.org/Solicitation.aspx
