Tittelmaster Mix For One-Step Real-Time Reverse Transcription Pcr (Rt-Qpcr) At Helse Bergen Hf And Helse Førde Hf . Descriptionthe Purpose Of The Procurement Is To Enter Into Regional Parallel Framework Agreements For The Purchase Of A Master Mix For One-Step Real-Time Reverse Transcription Pcr (Rt-Qpcr) For The Microbiology Department At Helse Bergen Hf And Helse Førde Hf. The Microbiology Department At Helse Bergen Hf And Helse Førde Hf Wants An Offer For Pcr Master Mix For The Detection Of Microbial Rna From Patient Samples (Real-Time Reverse Transcription Pcr/Rt-Qpcr). The Master Mix Will Mainly Be Used For The Detection Of Rna Viruses In Respiratory Samples And The Estimated Sample Volume At Helse Bergen Hf Is 350,000 – 450,000 Pcr Reactions Per Year. At Helse Førde Hf, The Estimated Sample Volume Is 60,000 – 70,000 Pcr Reactions Per Year. There Is A Need For A High-Quality Master Mix That Provides High Sensitivity, High Specificity And Stable Results Over Time. The Master Mix Must Be Optimized For Multiplexing And Not Sensitive To Any Pcr-Inhibiting Substances In The Eluate. Helse Bergen Uses Roches Flow Platform To Analyze The Respiratory Analyses, Which Consists Of Hamilton Robots And Light Cycler 480/Lightcycler Pro. At Helse Førde, Lightcycler480ii And Rotorgene Rgq Were Used. Finished Pipetted Pcr Trays Can Be Left In A Queue And Wait For A Free Pcr Instrument. The Reagents Must Therefore Be Able To Stand For Some Time In Light/Room Temperature After Pipetting. See Appendix 2 - Requirements Specification Sheet Tab 3. Minimum Requirements And 4. Quality, As Well As Appendix 2A - Test Form For A More Detailed Description. An Initial Test Will Be Carried Out At Helse Bergen Hf Of Master Mix Candidates With The Departments Primers/Probes For Relevant Agents On The Flow System. Master Mixers That Do Not Deliver Equivalent Or Better Results Than Todays Reagents (Ref. Minimum Requirements No. 7 And 8) Will Not Be Taken Into Further Consideration. The Master Mix Candidates Who Deliver Satisfactory Results Will Proceed To An Extended Trial At Both Helse Bergen Hf And Helse Førde Hf, If Based On The Established Award Criteria They Have The Opportunity To Emerge Victorious From The Tender.
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