A Set Of Reagents For Detecting Yersenia Pestis (Pla And Caf1) In Biological Material By The Pcr Method In Real Time, The Principle Of Analysis Is Based On The Registration Of The Amplification Of The Selected Specific Fragment Of The Yersinia Pestis Plam Ppst (Ppcp1) And Ppcp1) And The Pfra Caf1 Caf1 Gene (Pmt1) For Detecting Yersinia Pestis Strains, Devoid Of One Of The Specified Species -Specific Plasmide Ppst Or Pfra. Mixtures Of Deoxinucleotidrifospetes (Dntf), Fluorescent Probes And The Taq Polymerase Enzyme Are Hybridized With The Complementary Area Of Amplified Dna Targets, As A Result Of Which There Is An Increase In The Intensity Of Fluorescence. The Intensity Of A Flaure Signal. Real Time Signal. A Set Of Reagents Is Designed For Setting 96 Definitions In The Volume Of The Reaction Mixture Of 25 Μl, Including Control Samples. Perhaps 12 Independent Productions Of Pcr For 8 Definitions, Including Control Samples. 1. Description Of Reagents The Volume Of The Reagents Specified In The List May Have Permissible Error.
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