Expression of Interest For Stability Monitoring Of Gmo Crms

Expression of Interest for Stability Monitoring of GMO CRMs. Description: In the frame of the post certification monitoring of certified reference materials, measurements are required for the determination of the GMO content in GMO certified reference materials: On each of the GMO CRMS the following determination shall be performed: 4 bottles of the GMO material (1 g) will be provided by JRC Geel. For each bottle, three independent DNA extractions will be performed from three subsamples, for a total of 12 extracts. On each of the extracts, the following experiments must be performed: 1 gel electrophoresis to assess the integrity of the DNA. (NB: all the extracts can be run on the same gel). 1 rt-PCR measurement for the transgene and 1 rt-PCR for the endogene, except for Lot 7, where 3 rt-PCR measurements i.e; 2 for the transgene and 1 for the endogene are needed. All measurements will be performed under repeatability conditions i.e. in one analytical series. Gel electrophoresis (as much as necessary) with the rt-PCR results + adequate standards to verify/compare the size of the resulting band. Absorbance ratios of 260nm/280nm and 260nm/230nm. RNase has to be added during DNA extraction to digest possible RNA in the extract. This is to avoid a RNA band on the gel (low molecular weight). The materials can be analysed with other materials without impeding the independence of the results for the individual materials. In the frame of the post certification monitoring of certified reference materials, measurements are required for the determination of the GMO content in GMO certified reference materials: On each of the GMO CRMS the following determination shall be performed: 4 bottles of the GMO material (1 g) will be provided by JRC Geel. For each bottle, three independent DNA extractions will be performed from three subsamples, for a total of 12 extracts. On each of the extracts, the following experiments must be performed: 1 gel electrophoresis to assess the integrity of the DNA. (NB: all the extracts can be run on the same gel). 1 rt-PCR measurement for the transgene and 1 rt-PCR for the endogene, except for Lot 7, where 3 rt-PCR measurements i.e; 2 for the transgene and 1 for the endogene are needed. All measurements will be performed under repeatability conditions i.e. in one analytical series. Gel electrophoresis (as much as necessary) with the rt-PCR results + adequate standards to verify/compare the size of the resulting band. Absorbance ratios of 260nm/280nm and 260nm/230nm. RNase has to be added during DNA extraction to digest possible RNA in the extract. This is to avoid a RNA band on the gel (low molecular weight). The materials can be analysed with other materials without impeding the independence of the results for the individual materials. Tender Link : https://ec.europa.eu/info/funding-tenders/opportunities/portal/screen/opportunities/tender-details/e55e93c0-f5ff-43d8-b2bb-0cee8da21989-EXA

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