Rna Biomarkers For Predicting Radiation Injury For Radiation Biodosimetry

General Information Short Title Rna Biomarkers For Predicting Radiation Injury For Radiation Biodosimetry Document Type Notice Of Intent Solicitation Number 75N91021r00033 Posted Date 4 20 2021 Response Date 4 26 2021 Classification Code B599 Special Studies Analysis Other Naics Code 541380 Testing Laboratories Contracting Office Address Department Of Health And Human Services National Institutes Of Health National Cancer Institute Office Of Acquisitions 9609 Medical Center Drive Room 1E232 Bethesda Md 20892 United States Description The U S Department Of Health And Human Services Dhhs National Institutes Of Health Nih National Cancer Institute Nci Center For Cancer Research Ccr Radiation Oncology Branch Rob Experimental Therapeutics Section Ets Intends To Procure Services On A Sole Source Basis From Novogene Corportation Inc This Procurement Is Being Conducted Using Commercial Item Procedures Pursuant To Far Part 12 And Far Part 13 Specifically Far Subpart13 106 1 B 1 I Allowing The Contracting Officer Co To Solicit From One Source Only One Award Will Be Made As A Result Of This Solicitation This Will Be Awarded As A Severable Firm Fixed Price Type Contract It Has Been Determined There Are No Opportunities To Acquire Green Products Or Services For This Procurement Background Radiological Attacks And Nuclear Detonations Can Cause Mass Casualties Victims May Have Received Substantial Radiation Doses And May Not Immediately Exhibit Visible Symptoms Of Radiation Sickness Victims With Whole Body Or Substantial Partial Body Exposure Gt 2 Gy Will Require Immediate Treatment Within 24 Hours To Mitigate Radiation Injury While Others Will Require Both Intermediate And Long Term Management For Possible Injury To The Marrow Gastrointestinal Tract Lung And Other Organs Early Prediction Of Possible Acute Intermediate And Delayed Effects Will Enable Timely Therapeutic Interventions Which Will Not Only Reduce Death But Also Improve The Quality Of Life For The Victims The Nci Needs To Identify Radiation Induced Global Mirna Mrna Lncrna Expression Patterns After Different Doses And Time Points Following Partial Body Irradiation In A Non Human Primate Model A Specific Functional Mirna Expression Signature Previously Derived From Mouse Lung Tissue After Whole Body Irradiation From The Nci Laboratory Will Be Assessed In The Whole Blood And Serum Samples After Partial Body Irradiation In These Non Human Primate Model And Also Using A Human Organ On A Chip Model These Could Then Be Developed As Part Of The Point Of Care And High Through Put Screening Platforms That Are Under Development By Other Groups As Part Of Niaid And Barda Programs Objective The Primary Objective Of The Project Is To Obtain Microrna Long Non Coding Rna And Mrna Expression Information By Performing Rnaseq Analysis With The Following Workflow Preparation Of Cdna Libraries Rna Qc Before And After Library Prepration Performing Rna Seq On Illumina Platform Obtaining 20 Million Reads Sample Aligning The Reads To Reference Genome For Data Analysis With This Data The Nci Will Identify Biomarker Signatures Of Radiation Exposure And Of Organ Specific Radiation Injury Scope This Project Is A Multi Step Process With Each Step Dependent On The Successful Completion Of The Former The Process Starts With A Quality Check Of The Rna And Follows With Sequential Steps Of Rrna Depletion Globin Rna Depletion Adapter Ligation Cdna Preparation Sample Purification And Qc Reading On An Illumina Platform Aligning Reads To A Reference Genome And Finally Data Clean Up And Analysis For Identifying Differentially Expressed Coding And Non Coding Rnas Contract Requirements And Personnel Qualifications The Contractor Shall Perform The Following Tasks Mrna And Lncrna Sequencing Total Rna

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